Tooth Slice/Scaffold Model of Dental Pulp Tissue Engineering

Sakai, Vivien Thiemy; Cordeiro, Mabel Mariela Rodríguez; Dong, Zhihong; Zhang, Z.; Zeitlin, Benjamin David; Nör, Jacques E.

doi:10.1177/0022034511405325

Cited by 75 publications

(51 citation statements)

References 47 publications

(95 reference statements)

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“…Taken together, though electrospun scaffolds have not yet been tested in vivo in either human or animal pulpless models in Sakai et al, 2011;Casagrande et al, 2011;Rosa et al, 2013). immature permanent teeth, recent studies (Yang et al, 2010;Kim et al, 2014) positively highlight their regenerative potential from both an in vitro and an in vivo (i.e., subcutaneous model) standpoint.…”

Section: Bioactive Scaffolds For Pulp-dentin Complex Regenerationmentioning

confidence: 99%

Tissue-engineering-based Strategies for Regenerative Endodontics

et al. 2014

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Stemming from in vitro and in vivo pre-clinical and human models, tissue-engineering-based strategies continue to demonstrate great potential for the regeneration of the pulp-dentin complex, particularly in necrotic, immature permanent teeth. Nanofibrous scaffolds, which closely resemble the native extracellular matrix, have been successfully synthesized by various techniques, including but not limited to electrospinning. A common goal in scaffold synthesis has been the notion of promoting cell guidance through the careful design and use of a collection of biochemical and physical cues capable of governing and stimulating specific events at the cellular and tissue levels. The latest advances in processing technologies allow for the fabrication of scaffolds where selected bioactive molecules can be delivered locally, thus increasing the possibilities for clinical success. Though electrospun scaffolds have not yet been tested in vivo in either human or animal pulpless models in immature permanent teeth, recent studies have highlighted their regenerative potential both from an in vitro and in vivo ( i.e., subcutaneous model) standpoint. Possible applications for these bioactive scaffolds continue to evolve, with significant prospects related to the regeneration of both dentin and pulp tissue and, more recently, to root canal disinfection. Nonetheless, no single implantable scaffold can consistently guide the coordinated growth and development of the multiple tissue types involved in the functional regeneration of the pulp-dentin complex. The purpose of this review is to provide a comprehensive perspective on the latest discoveries related to the use of scaffolds and/or stem cells in regenerative endodontics. The authors focused this review on bioactive nanofibrous scaffolds, injectable scaffolds and stem cells, and pre-clinical findings using stem-cell-based strategies. These topics are discussed in detail in an attempt to provide future direction and to shed light on their potential translation to clinical settings.

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Section: Bioactive Scaffolds For Pulp-dentin Complex Regenerationmentioning

confidence: 99%

Tissue-engineering-based Strategies for Regenerative Endodontics

et al. 2014

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“…TGF-b1 may be involved in signaling events related to the proliferation, differentiation and recruitment of stem/progenitor cells to the site of tooth injury, in order to initiate regeneration, thus acting as a stimulating factor for dental tissue repair. 6,7,8,9,10,11,12 From a clinical perspective, the use of appropriate irrigation solutions could release the TGF-b1 stored in the dentin, thus contributing to successful regenerative endodontic treatment by directing cellular fate. 4 Two irrigants currently used during cleaning and shaping of the root canal are ethylenediaminetetraacetic acid (EDTA) 13 and sodium hypochlorite (NaOCl).…”

Section: Introductionmentioning

confidence: 99%

Effect of EDTA on TGF-β1 released from the dentin matrix and its influence on dental pulp stem cell migration

et al. 2016

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Bioactive molecules stored in dentin, such as transforming growth factor beta1 (TGF-b1), may be involved in the signaling events related to dental tissue repair. The authors conducted an in vitro evaluation of the amount of TGF-b1 released from dentin slices after treatment with 10% ethylenediaminetetraacetic acid (EDTA), 2.5% sodium hypochlorite (NaOCl) or phosphate-buffered saline (PBS), and the effect of this growth factor on stem cell migration from human exfoliated deciduous teeth (SHED). Sixty 1-mm-thick tooth slices were prepared with or without the predentin layer, and treated with either 10% EDTA for 1 minute, 2.5% NaOCl for 5 days or kept in PBS. Tooth slice conditioned media were prepared and used for TGF-b1 ELISA and migration assays. Culture medium with different concentrations of recombinant human TGF-b1 (0.5, 1.0, 5.0 or 10.0 ng/mL) was also tested by migration assay. The data were evaluated by ANOVA and Tukey's test. Optical density values corresponding to media conditioned by tooth slices either containing or not containing the predentin layer and treated with 10% EDTA were statistically greater than the other groups and close to 1 ng/mL. Increased rates of migration toward media conditioned by tooth slices containing the predentin layer and treated with PBS, 10% EDTA or 2.5% NaOCl were observed. Recombinant human TGF-b1 also stimulated migration of SHED, irrespective of the concentration used. EDTA may be considered an effective extractant of TGF-b1 from the dentin matrix. However, it does not impact SHED migration, suggesting that other components may account for the cell migration.

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“…Previous studies of solid polymer scaffolds have shown that differentiation of DPSC may be stimulated by the presence of dentine (Sakai et al, 2011). In our study, we used sterilised pulpless tooth slice incubated in culture medium to stimulate the odontogenic differentiation.…”

Section: Dpscs Odontogenic Differentiationmentioning

confidence: 99%

Biological and bactericidal properties of Ag-doped bioactive glass in a natural extracellular matrix hydrogel with potential application in dentistry

Wang

Chatzistavrou

Faulk

et al. 2015

eCM

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The aim of this study was the fabrication and evaluation of a novel bioactive and bactericidal material, which could have applications in dentistry by supporting tissue regeneration and killing oral bacteria. Our hypothesis was that a new scaffold for pulp-dentin tissue engineering with enhanced antibacterial activity could be obtained by associating extracellular matrix derived from porcine bladder with an antibacterial bioactive glass. Our study combines in vitro approaches and ectopic implantation in scid mice. The novel material was fabricated by incorporating a sol-gel derived silver (Ag)-doped bioactive glass (BG) in a natural extracellular matrix (ECM) hydrogel in ratio 1:1 in weight % (Ag-BG/ECM). The biological properties of the Ag-BG/ECM were evaluated in culture with dental pulp stem cells (DPSCs). In particular, cell proliferation, cell apoptosis, stem cells markers profile, and cell differentiation potential were studied. Furthermore, the antibacterial activity against Streptococcus mutans and Lactobacillus casei was measured. Moreover, the capability of the material to enhance pulp/dentin regeneration in vivo was also evaluated. Our data show that Ag-BG/ECM significantly enhances DPSCs' proliferation, it does not affect cell morphology and stem cells markers profile, protects cells from apoptosis, and enhances in vitro cell differentiation and mineralisation potential as well as in vivo dentin formation. Furthermore, Ag-BG/ECM strongly inhibits S. mutans and L. casei growth suggesting that the new material has also anti-bacterial properties. This study provides foundation for future clinical applications in dentistry. It could potentially advance the currently available options of dental regenerative materials.

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Tooth Slice/Scaffold Model of Dental Pulp Tissue Engineering

Cited by 75 publications

References 47 publications

Tissue-engineering-based Strategies for Regenerative Endodontics

Tissue-engineering-based Strategies for Regenerative Endodontics

Effect of EDTA on TGF-β1 released from the dentin matrix and its influence on dental pulp stem cell migration

Biological and bactericidal properties of Ag-doped bioactive glass in a natural extracellular matrix hydrogel with potential application in dentistry

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