Tooth regeneration from newly established cell lines from a molar tooth germ epithelium

Komine, Akihiko; Suenaga, Momoko; Nakao, Kazuhisa; Tsuji, Takashi; Tomooka, Yasuhiro

doi:10.1016/j.bbrc.2007.02.039

Cited by 48 publications

(46 citation statements)

References 25 publications

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“…This alternative may be tooth regeneration. Using animal study models, cells isolated from tooth buds can be seeded onto scaffolds and form ectopic teeth in vivo (58)(59)(60)(61)(62). Nakao et al (62) recently engineered teeth ectopically followed by transplantation into an othrotopic site in the mouse jaw.…”

Section: Scap For Bioroot Engineeringmentioning

confidence: 99%

The Hidden Treasure in Apical Papilla: The Potential Role in Pulp/Dentin Regeneration and BioRoot Engineering

Huang

Sonoyama

Liu

et al. 2008

Journal of Endodontics

650

543

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Some clinical case reports have shown that immature permanent teeth with periradicular periodontitis or abscess can undergo apexogenesis after conservative endodontic treatment. A call for a paradigm shift and new protocol for the clinical management of these cases has been brought to attention. Concomitantly, a new population of mesenchymal stem cells residing in the apical papilla of permanent immature teeth recently has been discovered and was termed stem cells from the apical papilla (SCAP). These stem cells appear to be the source of odontoblasts that are responsible for the formation of root dentin. Conservation of these stem cells when treating immature teeth may allow continuous formation of the root to completion. This article reviews current findings on the isolation and characterization of these stem cells. The potential role of these stem cells in the following respects will be discussed: (1) their contribution in continued root maturation in endodontically treated immature teeth with periradicular periodontitis or abscess and (2) their potential utilization for pulp/ dentin regeneration and bioroot engineering. KeywordsApexogenesis; apical papilla; bioroot engineering; dental pulp stem cells; immature teeth; periodontal ligament stem cells; pulp regeneration; stem cells from human exfoliated deciduous teeth; stem cells from the apical papilla A number of recent clinical case reports have revealed the possibilities that many teeth that traditionally would receive apexification may be treated for apexogenesis. A call for a paradigm shift and new protocol for the clinical management of these cases has been made by the authors (1-3). A recent scientific finding, which may explain in part why apexogenesis can occur in these infected immature permanent teeth, is the discovery and isolation of a new population of mesenchymal stem cells (MSCs) residing in the apical papilla of incompletely developed teeth (4,5). These cells are termed stem cells from the apical papilla (SCAP), and they differentiate

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Section: Scap For Bioroot Engineeringmentioning

confidence: 99%

The Hidden Treasure in Apical Papilla: The Potential Role in Pulp/Dentin Regeneration and BioRoot Engineering

Huang

Sonoyama

Liu

et al. 2008

Journal of Endodontics

650

543

View full text Add to dashboard Cite

show abstract

“…Ideally, this approach should provide a non-limited source of cells and introduce new genetic information to reprogram a non-dental cell to acquire odontogenic properties. For example, p53-deficient mice were used to establish dental epithelial clonal cell lines subsequently associated with mesenchymal cells to bioengineer teeth in vivo (Komine et al, 2007). These cell lines demonstrated heterogeneous outcomes in terms of regeneration depending on their differentiation state.…”

Section: Association Of Epithelial and Mesenchymal Stem Cellsmentioning

confidence: 99%

“…These cell lines demonstrated heterogeneous outcomes in terms of regeneration depending on their differentiation state. Although this technique provides us with an unlimited source of epithelial cells and shows the potential of genetically modified cells that can be used for tooth engineering (Komine et al, 2007), many questions have to be resolved. Which gene should be used to trigger an odontogenic program?…”

Section: Association Of Epithelial and Mesenchymal Stem Cellsmentioning

confidence: 99%

Stem cells for tooth engineering

Bluteau

Luder

Bari³

et al. 2008

eCM

168

152

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Tooth development results from sequential and reciprocal interactions between the oral epithelium and the underlying neural crest-derived mesenchyme. The generation of dental structures and/or entire teeth in the laboratory depends upon the manipulation of stem cells and requires a synergy of all cellular and molecular events that finally lead to the formation of tooth-specific hard tissues, dentin and enamel. Although mesenchymal stem cells from different origins have been extensively studied in their capacity to form dentin in vitro, information is not yet available concerning the use of epithelial stem cells. The odontogenic potential resides in the oral epithelium and thus epithelial stem cells are necessary for both the initiation of tooth formation and enamel matrix production. This review focuses on the different sources of stem cells that have been used for making teeth in vitro and their relative efficiency. Embryonic, post-natal or even adult stem cells were assessed and proved to possess an enormous regenerative potential, but their application in dental practice is still problematic and limited due to various parameters that are not yet under control such as the high risk of rejection, cell behaviour, long tooth eruption period, appropriate crown morphology and suitable colour. Nevertheless, the development of biological approaches for dental reconstruction using stem cells is promising and remains one of the greatest challenges in the dental field for the years to come.

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“…Epithelial Rests of Malassez (REM), present in mature human periodontal tissues, may be a suitable source for human dental epithelium [130]. In addition, current attempts to reprogram cell sources include the development of spontaneously derived immortalized murine dental epithelial cell lines, which when grown in low calcium-supplemented media, can express enamel markers such as amelogenin [131,132], and the use of single-cell suspensions generated from tooth germ tissue [133]. Lessons from these studies should assist efforts to generate suitable human dental epithelial cell lines from available tissues such as Hertwig's epithelial root sheath or epithelial cell REM.…”

Section: Unmet Needsmentioning

confidence: 99%