Tonsil-derived mesenchymal progenitor cells acquire a follicular dendritic cell phenotype under cytokine stimulation

Cho, Kyung Ah; Kim, Ji Yon; Kim, Hansu; Ryu, Kyung Ha; Woo, So Youn

doi:10.1016/j.cyto.2012.04.016

Cited by 23 publications

(20 citation statements)

References 9 publications

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“…T-MSCs were isolated from tonsils collected from patients during tonsillectomy, as previously described [19,21,42]. We isolated four different T-MSC lines from each of four patients.…”

Section: Methodsmentioning

confidence: 99%

Differentiation of Human Tonsil-Derived Mesenchymal Stem Cells into Schwann-Like Cells Improves Neuromuscular Function in a Mouse Model of Charcot-Marie-Tooth Disease Type 1A

Park

Jung

Myung

et al. 2018

IJMS

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Charcot-Marie-Tooth disease type 1A (CMT1A) is the most common inherited motor and sensory neuropathy, and is caused by duplication of PMP22, alterations of which are a characteristic feature of demyelination. The clinical phenotype of CMT1A is determined by the degree of axonal loss, and patients suffer from progressive muscle weakness and impaired sensation. Therefore, we investigated the potential of Schwann-like cells differentiated from human tonsil-derived stem cells (T-MSCs) for use in neuromuscular regeneration in trembler-J (Tr-J) mice, a model of CMT1A. After differentiation, we confirmed the increased expression of Schwann cell (SC) markers, including glial fibrillary acidic protein (GFAP), nerve growth factor receptor (NGFR), S100 calcium-binding protein B (S100B), glial cell-derived neurotrophic factor (GDNF), and brain-derived neurotrophic factor (BDNF), which suggests the differentiation of T-MSCs into SCs (T-MSC-SCs). To test their functional efficiency, the T-MSC-SCs were transplanted into the caudal thigh muscle of Tr-J mice. Recipients’ improved locomotive activity on a rotarod test, and their sciatic function index, which suggests that transplanted T-MSC-SCs ameliorated demyelination and atrophy of nerve and muscle in Tr-J mice. Histological and molecular analyses showed the possibility of in situ remyelination by T-MSC-SCs transplantation. These findings demonstrate that the transplantation of heterologous T-MSC-SCs induced neuromuscular regeneration in mice and suggest they could be useful for the therapeutic treatment of patients with CMT1A disease.

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“…T-MSCs were isolated from tonsils collected from patients during tonsillectomy, as previously described [19,21,42]. We isolated four different T-MSC lines from each of four patients.…”

Section: Methodsmentioning

confidence: 99%

Differentiation of Human Tonsil-Derived Mesenchymal Stem Cells into Schwann-Like Cells Improves Neuromuscular Function in a Mouse Model of Charcot-Marie-Tooth Disease Type 1A

Park

Jung

Myung

et al. 2018

IJMS

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“…Established methods were used for isolating T‐MSCs from tonsillar tissues . These were obtained during routine tonsillectomies and were minced and digested in Dulbecco's modified Eagle medium (DMEM; Invitrogen, Carlsbad, California) containing 210 U/ml collagenase type I (Invitrogen) and 10 μg/ml DNase (Sigma‐Aldrich, St. Louis, Missouri).…”

Section: Methodsmentioning

confidence: 99%

Application of differentiated human tonsil–derived stem cells to trembler‐J mice

et al. 2017

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“…FDCs are thought to be differentiated from stromal precursors of mesenchymal origin upon activation with lymphotoxin from B cells, as lymphotoxin stimulates the differentiation of T-MSCs into FDC-like cells [9]. TLR4 signaling modulates FDC activation and the germinal center reaction [15].…”

Section: Discussionmentioning

confidence: 99%

“…Stimulation with interleukin (IL)-4, granulocyte-macrophage colony-stimulating factor, or tumor necrosis factor (TNF)-a induced the differentiation of T-MSCs into the follicular dendritic cell (FDC) phenotype [9]. Because these T-MSCs were originally isolated from inflamed tonsillar tissues, we were curious about their activation status in response to innate immune stimuli, such as Toll-like receptors (TLRs).…”

Section: Introductionmentioning

confidence: 99%

Tonsil-derived mesenchymal stromal cells produce CXCR2-binding chemokines and acquire follicular dendritic cell-like phenotypes under TLR3 stimulation

et al. 2015

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Tonsil-derived mesenchymal progenitor cells acquire a follicular dendritic cell phenotype under cytokine stimulation

Cited by 23 publications

References 9 publications

Differentiation of Human Tonsil-Derived Mesenchymal Stem Cells into Schwann-Like Cells Improves Neuromuscular Function in a Mouse Model of Charcot-Marie-Tooth Disease Type 1A

Differentiation of Human Tonsil-Derived Mesenchymal Stem Cells into Schwann-Like Cells Improves Neuromuscular Function in a Mouse Model of Charcot-Marie-Tooth Disease Type 1A

Application of differentiated human tonsil–derived stem cells to trembler‐J mice

Tonsil-derived mesenchymal stromal cells produce CXCR2-binding chemokines and acquire follicular dendritic cell-like phenotypes under TLR3 stimulation

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