Tonglian Decoction (通莲汤) arrests the cell cycle in S-phase by targeting the nuclear factor-kappa B signal pathway in esophageal carcinoma Eca109 cells

Jia, Yongsen; Hu, Xueqin; Li, Jian; Szász, András; Hegyi, Gabriella; Han, Bing-Sheng

doi:10.1007/s11655-016-2096-3

Cited by 5 publications

(8 citation statements)

References 16 publications

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“…To explore the mechanisms underlying the antiproliferative activity of HMF in NCI-H1975 cells, we then detected the cell cycle distribution. Like the cell cycle arrest effect of some TCM [ 20 – 22 ], HMF could induce S phase cell cycle arrest. Sub-G0/G1 cells are the hallmark of apoptosis, and the flow cytometry results also confirmed the apoptosis inducing effect of HMF.…”

Section: Discussionmentioning

confidence: 99%

Haimufang decoction, a Chinese medicine formula for lung cancer, arrests cell cycle, stimulates apoptosis in NCI-H1975 cells, and induces M1 polarization in RAW 264.7 macrophage cells

Xu³

et al. 2020

BMC Complement Med Ther

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Background: Lung cancer has the highest morbidity and mortality in the world and novel treatment strategies are still needed. Haimufang decoction (HMF) is a patented clinical prescription of traditional Chinese medicine for lung cancer treatment. HMF is composed of four herbs and has been applied clinically in advanced cancer patients. However, its therapeutic mechanisms are still unclear. This study aims to elucidate the possible mechanisms of HMF for the treatment of lung cancer. Methods: 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay was applied for evaluating the proliferative effect of HMF in lung cancer cells and monocyte macrophage RAW264.7 cells. Flow cytometer was used to detect the effects of HMF on cell cycle and apoptosis, and western blotting was employed to explore the potential apoptotic mechanisms of HMF on lung cancer cells. For immunomodulatory effect, co-culture system was used to detect the activation of macrophage RAW264.7 cells when treated with HMF, and neutral red assay was used to measure the effect of HMF on the phagocytosis of the activated macrophages. Enzyme linked immunosorbent assay, flow cytometer, and immunofluorescence staining method were employed for the investigation on the underlying mechanisms of the immunomodulatory effect on RAW264.7 induced by HMF.

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Section: Discussionmentioning

confidence: 99%

Haimufang decoction, a Chinese medicine formula for lung cancer, arrests cell cycle, stimulates apoptosis in NCI-H1975 cells, and induces M1 polarization in RAW 264.7 macrophage cells

Xu³

et al. 2020

BMC Complement Med Ther

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“…[17][18][19][20][21] In this article, we review the application status and mechanistic research of TCM and discuss its shortcomings and prospects in the context of integrated medicine, precision medicine, and evidencebased medicine. [22][23][24][25][26] Ancient Classical Formulas for the Treatment of EC Although EC itself was not described in ancient China, TCM was applied to improve clinical symptoms such as dysphagia. 27,28 Meanwhile, ancient Chinese herbal formulas gradually developed from single herbs to multiple drugs (formulas).…”

Section: Introductionmentioning

confidence: 99%

“… 17 - 21 In this article, we review the application status and mechanistic research of TCM and discuss its shortcomings and prospects in the context of integrated medicine, precision medicine, and evidence-based medicine. 22 - 26 …”

Section: Introductionmentioning

confidence: 99%

Traditional Chinese Medicine Therapy for Esophageal Cancer: A Literature Review

et al. 2021

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Esophageal cancer (EC) is the sixth leading cause of cancer-related deaths worldwide. Western medicine has played a leading role in its treatment, but its prognosis remains unsatisfactory. Therefore, the development of effective therapies is important. Traditional Chinese medicine (TCM) has been practiced for thousands of years, and involves taking measures before diseases occur, deteriorate, and recur. Interestingly, there is growing evidence that TCM can improve the therapeutic effects in reversing precancerous lesions, inhibiting the recurrence and metastasis of EC. In this article, we review traditional Chinese herbs and formulas that have preventive and therapeutic effects on EC, summarize the application and research status of TCM in patients with EC, and discuss its shortcomings and prospects in the context of translational, evidence-based, and precision medicine.

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“…Based on the formula and TCM pharmacology theory, MTD was invented by our research team and patented in China [ 12 ]. A series of basic experiments and clinical trials were conducted showing that MTD inhibited Eca109 cell proliferation and downregulated the NF- κ B signaling pathway [ 13 ]. The present study explored the anticancer effects of MTD on the invasion and metastasis of Eca109 cells in the hypoxic microenvironment, aiming to elucidate the molecular mechanisms of the effects of MTD on EMT mediated by HIF-1 α -Snail axis.…”

Section: Introductionmentioning

confidence: 99%

Anticancer Activity of Modified Tongyou Decoction on Eca109 Esophageal Cancer Cell Invasion and Metastasis through Regulation of the Epithelial‐Mesenchymal Transition Mediated by the HIF‐1α‐Snail Axis

Jia

Yan

Cao

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. To explore the activity of Modified Tongyou Decoction (MTD) against Eca109 esophageal cancer (EC) cell invasion and metastasis and to ascertain the mechanism of its anticancer activity during the epithelial-mesenchymal transition (EMT) as mediated by the HIF-1α-Snail axis. Methods. Herbal compounds were prepared by ethanol extraction, and 6 herbs composing into MTD were dipped in water-free ethanol and filtered. The filtrate was collected and centrifuged. The remains were concentrated into a paste which was adjusted to 5000mg/mL concentration with DMSO. PBS was used to dilute the herbal solution to the half maximal inhibitory concentration. A hypoxic microenvironment was induced with CoCl2 in RPMI 1640 medium, in which Eca109 cells were cultured. The cytotoxicity of MTD was determined with CCK-8 assay. The activity of MTD against cell invasion and metastasis was explored with scratch assay and transwell assay. Western blot analysis was conducted to analyze the anticancer effects of MTD on the expression of HIF-1α-Snail axis- and EMT-related proteins. Quantitative RT-PCR was used to assess the mRNA expression of Snail. Immunofluorescence labeling was performed to examine how MTD affected the coexpression of Snail and HIF-1α. Results. The fifty percent inhibitory dose of MTD was 1410 μg/mL in the normoxic environment and 1823 μg/mL in the hypoxic environment based on the CCK-8 assay. The scratch assay showed that MTD significantly inhibited cell migration in both the normoxic and hypoxic microenvironments compared with the control groups ( P < 0.05). The transwell assay showed that MTD significantly inhibited cell invasion in both the normoxic and hypoxic environments compared with the control groups ( P < 0.05). Western blot showed that MTD significantly inhibited the expression of the HIF-1α, Snail, Vimentin, MMP-2, MMP-9, and VE-cadherin proteins and significantly induced the expression of E-cadherin in both the normoxic and hypoxic microenvironments compared with the control groups ( P < 0.05). qRT-PCR indicated that MTD significantly inhibited Snail mRNA expression compared with that in the control groups ( P < 0.05). Immunofluorescence assay showed that MTD significantly inhibited the coexpression of HIF-1α and Snail in both the normoxic and hypoxic microenvironments compared with the control groups ( P < 0.05). Conclusion. MTD downregulated HIF-1α-Snail axis- and EMT-related proteins to inhibit EC cell invasion and metastasis in both the normoxic and hypoxic environments.

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Tonglian Decoction (通莲汤) arrests the cell cycle in S-phase by targeting the nuclear factor-kappa B signal pathway in esophageal carcinoma Eca109 cells

Cited by 5 publications

References 16 publications

Haimufang decoction, a Chinese medicine formula for lung cancer, arrests cell cycle, stimulates apoptosis in NCI-H1975 cells, and induces M1 polarization in RAW 264.7 macrophage cells

Haimufang decoction, a Chinese medicine formula for lung cancer, arrests cell cycle, stimulates apoptosis in NCI-H1975 cells, and induces M1 polarization in RAW 264.7 macrophage cells

Traditional Chinese Medicine Therapy for Esophageal Cancer: A Literature Review

Anticancer Activity of Modified Tongyou Decoction on Eca109 Esophageal Cancer Cell Invasion and Metastasis through Regulation of the Epithelial‐Mesenchymal Transition Mediated by the HIF‐1α‐Snail Axis

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