A B S T R A C TWe present the results obtained by processing high-resolution seismic data acquired along the spring line located in the Friuli-Venezia Giulia plain (NE of Italy), in order to characterize an important multilayered aquifer. This system is made of an unconfined layer and, at increasing depths, of several confined aquifers of variable thickness and hydraulic permeability, mainly consisting of sand and gravel material. The main targets of this study are two shallow aquifers located at about 30 m and 200 m depth respectively. The seismic method is not frequently used for this type of study but in this case, it was considered a good tool due to the depth of the targets. The detailed velocity model we obtained reveals lateral velocity variations with a maximum value of 600 m/s. The higher velocities could be associated to layers that are confined aquifers; in fact, sand and gravel are characterized by higher seismic velocity compared to clay layers. Pre-stack depth migration using this velocity model gives a clear picture of the multilayered aquifer, highlighting lateral changes of seismic amplitude along the main reflectors. Finally, vertical variations of Poisson's ratio, computed by amplitude versus offset analysis, provide useful information about the petrophysical properties, such as the fluid content of the subsoil and lithologic changes.