Tomato WRKY81 acts as a negative regulator for drought tolerance by modulating guard cell H2O2–mediated stomatal closure

Ahammed, Golam Jalal; Li, Xin; Yang, Youxin; Liu, Chaochao; Zhou, Guozhi; Wan, Hongjian; Cheng, Yuan

doi:10.1016/j.envexpbot.2019.103960

Cited by 93 publications

(41 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Low temperature induced increased NO accumulation and decreased stomatal conductance in cucumber plants (Zhang Z. W. et al, 2019). In tomatoes, rapid and increased stomatal closure was co-related with drought tolerance in the SlWRKY81silenced plants (Ahammed et al, 2019). Here, we found that increased wax content and stomatal conductance alleviated propamocarb residue levels in the CsHMGB-overexpressed plants (Figures 8B,C).…”

Section: Discussionsupporting

confidence: 50%

Overexpression of CsHMGB Alleviates Phytotoxicity and Propamocarb Residues in Cucumber

Xin

Luan

et al. 2020

Front. Plant Sci.

View full text Add to dashboard Cite

Cucumber (Cucumis sativus L.) is one of the most economically important fruits of the Cucurbitaceae family, therefore consideration of potential pesticide residues in the fruit in the context of cucumber breeding and production programs is important. Propamocarb (a pesticide commonly used to prevent downy mildew) is widely used in cucumber cultivation, but the molecular mechanism underlying the degradation and metabolism of propamocarb in cucumber is not well understood. We screened a candidate CsHMGB gene (CsaV3-5G28190) for response to propamocarb exposure using transcriptome data. The coding region of CsHMGB was 624 bp in length and encoded the conserved HMB-box region. CsHMGB expression differed significantly between the "D0351" genotype, which accumulated low levels of propamocarb, and the "D9320" genotype, which accumulated high levels of propamocarb. CsHMGB expression was positively correlated with propamocarb levels in the cucumber peel. CsHMGB expression was upregulated in the fruit peels of the "D0351" genotype following exposure to propamocarb stress for 3-120 h, but no difference was observed in expression between propamocarb treatment and control for the "D9320" genotype. For the "D0351" genotype, CsHMGB expression was higher in the fruit peels and leaves than that in female flowers; expression was moderate in the stems and fruit pulps, and weak in male flowers and roots. The CsHMGB protein was targeted to the nucleus in Arabidopsis protoplasts and in the epidermis of Nicotiana benthamiana leaves. We measured MDA, O 2 − , and H 2 O 2 levels in cucumber plants and found that they were likely to accumulate reactive oxygen species (ROS) in response to propamocarb stress. Analysis of antioxidant enzyme activity (SOD, POD, CAT, APX, GPX, GST, and GR) and the ascorbate-glutathione (AsA-GSH) system showed that the resistance of the plants was reduced and the levels of propamocarb residue was increased in CsHMGBsilenced plants in response to propamocarb stress. Conversely, overexpression of CsHMGB promoted glutathione-dependent detoxification by AsA-GSH system and improved the antioxidant potential, reduced the accumulation of ROS. Ultimately, the metabolism of propamocarb in cucumber was increased via increase in the wax levels and the stomatal conductance.

show abstract

Section: Discussionsupporting

confidence: 50%

Overexpression of CsHMGB Alleviates Phytotoxicity and Propamocarb Residues in Cucumber

Xin

Luan

et al. 2020

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Shade tolerant varieties maintain a lower MDA content and higher SOD, POD, and CAT activity and soluble protein content 50 . Moreover, the MDA content was significantly increased and the activities of SOD and CAT were significantly reduced after a PEG treatment 49 , which may have been due to the drought-induced accumulation of H 2 O 2 in the guard cells 53 . In this study, the low light treatments significantly increased the SOD activity and MDA content in both rice varieties.…”

Section: Discussionmentioning

confidence: 98%

Light and water treatment during the early grain filling stage regulates yield and aroma formation in aromatic rice

Liang

et al. 2020

Sci Rep

View full text Add to dashboard Cite

The effect of light and water on aromatic rice remain largely unclear. A pot experiment was conducted to investigate the influences of light-water treatments (CK: natural light and well-watered conditions, WS: natural light and water-stressed conditions, LL: low light and well-watered conditions, LL-WS: low light and water-stressed treatment) on yield and 2-acetyl-1-pyrroline (2AP) formation in aromatic rice. Compared with CK, the light-water treatments decreased grain yield (10.32–39.19%) due to reductions in the filled grain percentage and total dry weight, in the regulation of biomass distribution, and in the attributes of gas exchange and antioxidant response parameters. The 2AP content in grains increased in the LL treatment (5.08–16.32%) but decreased in the WS treatment compared with that in CK. The changes in 2AP were associated with changes in 2AP formation-related traits and element content. Low light and water stress led to yield declines in aromatic rice, but low light alleviated the decrease in 2AP content caused by water stress.

show abstract

“…Overexpression of BdWRKY36 in tobacco reduces the accumulation of ROS, activated NtLEA5, NtNCED1, and NtDREB3 in the ABA biosynthetic pathway, and significantly enhances the drought resistance of plants [48]. In Solanum lycopersicum, SlWRKY81 increases the drought tolerance of plants by inhibiting the accumulation of H 2 O 2 , playing a negative regulation role of stomatal closure [72].…”

Section: Wrky Tfs and Drought Stressmentioning

confidence: 99%

Function and Mechanism of WRKY Transcription Factors in Abiotic Stress Responses of Plants

Pang

et al. 2020

Plants

174

View full text Add to dashboard Cite

The WRKY gene family is a plant-specific transcription factor (TF) group, playing important roles in many different response pathways of diverse abiotic stresses (drought, saline, alkali, temperature, and ultraviolet radiation, and so forth). In recent years, many studies have explored the role and mechanism of WRKY family members from model plants to agricultural crops and other species. Abiotic stress adversely affects the growth and development of plants. Thus, a review of WRKY with stress responses is important to increase our understanding of abiotic stress responses in plants. Here, we summarize the structural characteristics and regulatory mechanism of WRKY transcription factors and their responses to abiotic stress. We also discuss current issues and future perspectives of WRKY transcription factor research.

show abstract

Tomato WRKY81 acts as a negative regulator for drought tolerance by modulating guard cell H2O2–mediated stomatal closure

Cited by 93 publications

References 33 publications

Overexpression of CsHMGB Alleviates Phytotoxicity and Propamocarb Residues in Cucumber

Overexpression of CsHMGB Alleviates Phytotoxicity and Propamocarb Residues in Cucumber

Light and water treatment during the early grain filling stage regulates yield and aroma formation in aromatic rice

Function and Mechanism of WRKY Transcription Factors in Abiotic Stress Responses of Plants

Contact Info

Product

Resources

About