Tomato Spotted Wilt Virus

Best, Rupert J

doi:10.1016/s0065-3527(08)60251-1

Cited by 132 publications

(63 citation statements)

References 67 publications

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“…In the past, T S W V isolates have been distinguished by s y m p t o m s on tomatoes (Norris, 1946) or on a few differential host plants (Best & Gallus, 1955). Antisera have rarely been used to study the serological relationships of different isolates of TSWV.…”

Section: Discussionmentioning

confidence: 99%

“…Although TSWV can easily be distinguished from other plant viruses, methods by which different isolates can readily be characterized are not available. Identification of the various isolates using symptoms in different indicator plants, as advocated by Norris (1946) and by Best & Gallus (1955), has not widely been used. Also, serology has not been explored extensively in the diagnosis and identification of TSWV and differentiation of the various isolates since antisera produced against TSWV often lack the required reliability or sensitivity (Paliwal, 1974;Reddy & Wightman, 1988;Tas et al, 1977).…”

Section: Introductionmentioning

confidence: 99%

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Serological Differentiation of 20 Isolates of Tomato Spotted Wilt Virus

Ávila

Huguenot

Resende

et al. 1990

Journal of General Virology

134

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Twenty tomato spotted wilt virus (TSWV) isolates were serologically compared in ELISA employing five different procedures using a rabbit polyclonal antiserum against nucleocapsid proteins (NuAb R) and mouse monoclonal antibodies (MAbs), two directed to nucleocapsid proteins (N1 and N2) and four directed to glycoproteins G1 to G4. All the antisera were raised against TSWV-CNPH~. The 20 isolates were differentiated into two distinct serogroups. Serogroup I consisting of 16 isolates strongly reacted with NuAb R. The other four isolates were poorly recognized by NuAb ~ and were placed in another serogroup, designated II. The panel of MAbs differentiated the TSWV isolates into three serotypes. The 16 isolates forming serogroup I reacted strongly with the MAbs generated and were identified as serotype I isolates. The four isolates which made up serogroup II were split into serotypes II and III. The serotype II isolates did not respond or responded poorly with MAbs N1, N2 and G3. The two other isolates placed in serotype III were recognized by N 1 but not by N2 and G3. Two isolates became defective after several mechanical passages and failed to respond or responded very poorly with MAbs directed to glycoproteins. Our results show that ELISA employing polyclonal and monoclonal antisera is a useful tool to differentiate TSWV isolates and to detect defective forms. The results also strongly suggest that TSWV nucleocapsid proteins are less conserved than the glycoproteins.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Serological Differentiation of 20 Isolates of Tomato Spotted Wilt Virus

Ávila

Huguenot

Resende

et al. 1990

Journal of General Virology

134

View full text Add to dashboard Cite

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“…Historically, thrips have played a conspicuous role in Australian entomology, considering the impact on ecological theory of the long-term population studies on Thrips imaginis (Andrewartha & Birch 1954), and the pioneering studies on thrips as vectors of the damaging tospovirus infections of crops (Best 1968). Recent years have seen a range of studies on Thysanoptera in Australia that involve various biological disciplines, such as pollination, as well as other aspects of host-plant associations, including coevolution and host capture within particular thrips lineages.…”

Section: Introductionmentioning

confidence: 99%

Australian Thysanoptera – biological diversity and a diversity of studies

Mound

2004

Australian Journal of Entomology

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Studies in Australia on thrips have had extensive impacts worldwide. In behaviour, the latest definition of eusociality is derived from work on the radiation of thrips on Acacia species in central Australia, and these Acacia thrips also having been used to develop the concept of 'model clades' for analysing the evolution of behavioural and ecological diversity. In ecology, the concept of the lack of density dependent factors in population dynamics was elaborated through studies on the plague thrips of southern Australia. In virology, thrips were first shown in Australia to be the vectors of tospoviruses, although these viruses, their vectors and the plants attacked are all non-native to this continent. Work in Australia has included the development of electronic methods of illustration, identification and information transfer about thrips, including the use of molecular methods for pest species recognition, and considerable advances have been made in Australia in our knowledge of the relationships between thrips and plants, from polyphagy to pollination.

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“…Thrips are the only known vectors of tospoviruses, causing widespread economic losses worldwide (Allen and Matteoni, 1988;Best andGallus, 1953, Peters et al, 1991). With no insect cell line suitable for the growth and study of tospoviruses, and to increase our understanding of the virus-vector relationships, a thrips tissue culture system was established.…”

Section: Introductionmentioning

confidence: 99%

Formulation of an Insect Medium for Thrips Monolayer Cell Cultures (Thysanoptera: Thripidae:Frankliniella occidentalis)

Hunter

Hsu

1996

Journal of Invertebrate Pathology

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A thrips tissue culture system was developed from embryonic eggs from Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae). Cell lines that have been established for other insects offer a wide range of usages for studies of insect physiology, toxicology, and pathology. This study examined the efficiency of different insect cell culture media for suitability in establishing a thrips cell culture system. Final development of a medium for thrips cell culture allowed cells to be cultured up to 7 months. Within 1-2 days after being explanted, tissue fragments and individual cells attached to the substrate; at this stage, fibroblast-like cells were the dominant cell type. Monolayers of epithelial-like cells were observed at 10-20 days. This is the first cell culture system established which provides a method for the examination of thrips cell growth and metabolism and which provides a method for the study of virus infection and replication within the thrips vector F. occidentalis.

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Tomato Spotted Wilt Virus

Cited by 132 publications

References 67 publications

Serological Differentiation of 20 Isolates of Tomato Spotted Wilt Virus

Serological Differentiation of 20 Isolates of Tomato Spotted Wilt Virus

Australian Thysanoptera – biological diversity and a diversity of studies

Formulation of an Insect Medium for Thrips Monolayer Cell Cultures (Thysanoptera: Thripidae:Frankliniella occidentalis)

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