Tomato SlYTH1 encoding a putative RNA m6A reader affects plant growth and fruit shape

Yin, Shuangqin; Ao, Qiujing; Qiu, Tiaoshuang; Tan, Caiyun; Tu, Yun; Kuang, Tianyin; Yang, Yingwu

doi:10.1016/j.plantsci.2022.111417

Cited by 13 publications

(15 citation statements)

References 46 publications

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“…In nearly all cases, these phenotypes are exacerbated by additional mutation of ECT4 . Recent studies report similar roles of rice and tomato YTHDF proteins in plant development (Yin et al, 2022;Ma et al, 2022). m 6 A is also found in viral mRNAs, first seen as early as 1975 in simian virus 40 mRNAs (Lavi & Shatkin, 1975).…”

Section: Introductionmentioning

confidence: 77%

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Plant YTHDF proteins are direct effectors of antiviral immunity against an m⁶A-containing RNA virus

Martínez-Pérez

Aparicio

Arribas-Hernández

et al. 2022

Preprint

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SUMMARYIn virus-host interactions, nucleic acid-directed first lines of defense that allow viral clearance without compromising growth are of paramount importance. Plants use the RNA interference pathway as such a basal antiviral immune system, but additional RNA-based mechanisms of defense also exist. The infectivity of the plant positive strand RNA virus alfalfa mosaic virus (AMV) relies on demethylation of viral RNA by recruitment of the cellularN6-methyladenosine (m6A) demethylase ALKBH9B, but how demethylation of viral RNA promotes AMV replication remains unknown. Here, we show that inactivation of the cytoplasmic YT521-B homology domain (YTH)-containing m6A-binding proteins, ECT2, ECT3, and ECT5 is sufficient to restore AMV infectivity in partially resistantalkbh9bmutants. We also show that the antiviral function of ECT2 is distinct from its previously demonstrated function in promotion of proliferation of primordial cells, because an ECT2 mutant carrying a small deletion in its intrinsically disordered region is partially compromised for antiviral defense, but not for developmental functions. These results indicate that the m6A-YTH axis constitutes a novel branch of basal antiviral immunity in plants.

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Section: Introductionmentioning

confidence: 77%

“…In nearly all cases, these phenotypes are exacerbated by additional mutation of ECT4 (Arribas-Hernández et al , 2020, 2018). Recent studies report similar roles of rice and tomato YTHDF proteins in plant development (Yin et al , 2022; Ma et al , 2022).…”

Section: Introductionmentioning

confidence: 79%

Plant YTHDF proteins are direct effectors of antiviral immunity against an m⁶A-containing RNA virus

Martínez-Pérez

Aparicio

Arribas-Hernández

et al. 2022

Preprint

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“…Recent research has demonstrated that the activity of ‘writers’, ‘readers’, and ‘erasers’ is vital for plant growth and abiotic stress responses ( Hu et al., 2019 ; Reichel et al., 2019 ) even though our understanding of the roles of such factors in plants is far behind that for their animal counterparts. Only recently, putative RNA m 6A reader- SlYTH1 has been shown to be involved in regulating multiple physiological processes in tomato, including seed germination, biosynthesis of gibberellin and seedling root formation ( Yin et al., 2022 ).…”

Section: Discussion: Modification Of M 6 a Rna And...mentioning

confidence: 99%

“…It occurs when a methyl group is attached to the nitrogen-containing base at the sixth position of the adenine residue of RNA ( Figure 1 ) ( Batista et al., 2014 ; Shen et al., 2021 ). Current research reveals that one of the major processes in RNA post-transcriptional regulation, the m 6 A modification, controls RNA fate ( Guo et al., 2022 ; Kumar and Mohapatra, 2021 ; Yin et al., 2022 ).…”

Section: Understanding Induced Senescence and Its Economic Importance...mentioning

confidence: 99%

N6-methyladenosine (m6A) RNA modification as a metabolic switch between plant cell survival and death in leaf senescence

et al. 2023

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Crop losses caused by climate change and various (a)biotic stressors negatively affect agriculture and crop production. Therefore, it is vital to develop a proper understanding of the complex response(s) to (a)biotic stresses and delineate them for each crop plant as a means to enable translational research. In plants, the improvement of crop quality by m6A editing is believed to be a promising strategy. As a reaction to environmental changes, m6A modification showed a high degree of sensitivity and complexity. We investigated differences in gene medleys between dark-induced leaf senescence (DILS) and developmental leaf senescence in barley, including inter alia RNA modifications active in DILS. The identified upregulated genes in DILS include RNA methyltransferases of different RNA types, embracing enzymes modifying mRNA, tRNA, and rRNA. We have defined a decisive moment in the DILS model which determines the point of no return, but the mechanism of its control is yet to be uncovered. This indicates the possibility of an unknown additional switch between cell survival and cell death. Discoveries of m6A RNA modification changes in certain RNA species in different stages of leaf senescence may uncover the role of such modifications in metabolic reprogramming. Nonetheless, there is no such data about the process of leaf senescence in plants. In this scope, the prospect of finding connections between the process of senescence and m6A modification of RNA in plants seems to be compelling.

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“…In nearly all cases, these phenotypes are exacerbated by additional mutation of ECT4 (Arribas-Hern andez et al, 2018, 2020), and five additional Arabidopsis YTHDF proteins (ECT5, ECT6, ECT7, ECT8, ECT10) share the molecular functions of ECT2/3 required for primordial cell proliferation, as seen by complementation of ect2/ect3/ect4 phenotypes upon ectopic expression in the ECT2/3 expression domain (preprint: Flores-T ellez et al, 2023). Stimulation of growth is a recurrent function of plant YTHDF proteins because similar roles have been found for rice and tomato YTHDF proteins (Ma et al, 2022;Yin et al, 2022), and because the sole YTHDF protein of the liverwort Marchantia polymorpha can replace ECT2/3 function when expressed in Arabidopsis (preprint: Flores-T ellez et al, 2023). m 6 A is also found in viral mRNAs, first seen as early as 1975 in simian virus 40 mRNAs (Lavi & Shatkin, 1975).…”

Section: Introductionmentioning

confidence: 89%

Plant YTHDF proteins are direct effectors of antiviral immunity against an N6‐methyladenosine‐containing RNA virus

et al. 2023

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In virus–host interactions, nucleic acid‐directed first lines of defense that allow viral clearance without compromising growth are of paramount importance. Plants use the RNA interference pathway as a basal antiviral immune system, but additional RNA‐based mechanisms of defense also exist. The infectivity of a plant positive‐strand RNA virus, alfalfa mosaic virus (AMV), relies on the demethylation of viral RNA by the recruitment of the cellular N6‐methyladenosine (m6A) demethylase ALKBH9B, but how demethylation of viral RNA promotes AMV infection remains unknown. Here, we show that inactivation of the Arabidopsis cytoplasmic YT521‐B homology domain (YTH)‐containing m6A‐binding proteins ECT2, ECT3, and ECT5 is sufficient to restore AMV infectivity in partially resistant alkbh9b mutants. We further show that the antiviral function of ECT2 is distinct from its previously demonstrated function in the promotion of primordial cell proliferation: an ect2 mutant carrying a small deletion in its intrinsically disordered region is partially compromised for antiviral defense but not for developmental functions. These results indicate that the m6A‐YTHDF axis constitutes a novel branch of basal antiviral immunity in plants.

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Tomato SlYTH1 encoding a putative RNA m6A reader affects plant growth and fruit shape

Cited by 13 publications

References 46 publications

Plant YTHDF proteins are direct effectors of antiviral immunity against an m⁶A-containing RNA virus

Plant YTHDF proteins are direct effectors of antiviral immunity against an m⁶A-containing RNA virus

N6-methyladenosine (m6A) RNA modification as a metabolic switch between plant cell survival and death in leaf senescence

Plant YTHDF proteins are direct effectors of antiviral immunity against an N6‐methyladenosine‐containing RNA virus

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Tomato SlYTH1 encoding a putative RNA m6A reader affects plant growth and fruit shape

Cited by 13 publications

References 46 publications

Plant YTHDF proteins are direct effectors of antiviral immunity against an m6A-containing RNA virus

Plant YTHDF proteins are direct effectors of antiviral immunity against an m6A-containing RNA virus

N6-methyladenosine (m6A) RNA modification as a metabolic switch between plant cell survival and death in leaf senescence

Plant YTHDF proteins are direct effectors of antiviral immunity against an N6‐methyladenosine‐containing RNA virus

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Plant YTHDF proteins are direct effectors of antiviral immunity against an m⁶A-containing RNA virus

Plant YTHDF proteins are direct effectors of antiviral immunity against an m⁶A-containing RNA virus