Tomato SlSAP3, a member of the stress‐associated protein family, is a positive regulator of immunity against Pseudomonas syringae pv. tomato DC3000

Liu, Shixia; Wang, Jiali; Jiang, Siyu; Wang, Hui; Gao, Yizhou; Zhang, Huijuan; Li, Dayong; Song, Fengming

doi:10.1111/mpp.12793

Cited by 32 publications

(23 citation statements)

References 49 publications

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“…SlSAP3 and SlSAP4 have recently been shown to be positive regulators of immunity, to Pseudomonas syringae pv. tomato ( Pst DC3000) and Botrytis cinerea (Liu et al ., 2019a, b). SlSAP3 silencing decreased the Pst DC3000‐induced expression of SA signalling and defence genes and attenuated immunity to Pst DC3000, whereas SlSAP3 overexpression in transgenic tomato increased them.…”

Section: Discussionmentioning

confidence: 99%

The root‐knot nematode effector MiPDI1 targets a stress‐associated protein (SAP) to establish disease in Solanaceae and Arabidopsis

et al. 2020

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Large amounts of effectors are secreted by the oesophageal glands of plant-parasitic nematodes, but their molecular mode of action remains largely unknown. We characterized a Meloidogyne incognita protein disulphide isomerase (PDI)-like effector protein (MiPDI1) that facilitates nematode parasitism. In situ hybridization showed that MiPDI1 was expressed specifically in the subventral glands of M. incognita. It was significantly upregulated during parasitic stages. Immunolocalization demonstrated MiPDI1 secretion in planta during nematode migration and within the feeding cells. Host-induced silencing of the MiPDI1 gene affected the ability of the nematode to infect the host, whereas MiPDI1 expression in Arabidopsis increased susceptibility to M. incognita, providing evidence for a key role of MiPDI1 in M. incognita parasitism. Yeast two-hybrid, bimolecular fluorescence complementation and coimmunoprecipitation assays showed that MiPDI1 interacted with a tomato stress-associated protein (SlSAP12) orthologous to the redox-regulated AtSAP12, which plays an important role in plant responses to abiotic and biotic stresses. SAP12 silencing or knocking out in Nicotiana benthamiana and Arabidopsis increased susceptibility to M. incognita. Our results suggest that MiPDI1 acts as a pathogenicity factor promoting disease by finetuning SAP-mediated responses at the interface of redox signalling, defence and stress acclimation in Solanaceae and Arabidopsis.

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Section: Discussionmentioning

confidence: 99%

The root‐knot nematode effector MiPDI1 targets a stress‐associated protein (SAP) to establish disease in Solanaceae and Arabidopsis

et al. 2020

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“…The tomato SlSAP3 gene positively regulates immunity against Pseudomonas syringae pv. tomato ( Pst ) DC3000 (Liu et al, 2019). The results indicate that soybean SAP genes may be involved in biotic stress responses.…”

Section: Discussionmentioning

confidence: 99%

Genomic Analysis of Stress Associated Proteins in Soybean and the Role of GmSAP16 in Abiotic Stress Responses in Arabidopsis and Soybean

et al. 2019

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Stress associated proteins (SAPs) containing A20/AN1 zinc finger domains have emerged as novel regulators of stress responses. In this study, 27 SAP genes were identified in soybean. The phylogenetic relationships, exon–intron structure, domain structure, chromosomal localization, putative cis-acting elements, and expression patterns of SAPs in various tissues under abiotic stresses were analyzed. Among the soybean SAP genes, GmSAP16 was significantly induced by water deficit stress, salt, and abscisic acid (ABA) and selected for further analysis. GmSAP16 was located in the nucleus and cytoplasm. The overexpression of GmSAP16 in Arabidopsis improved drought and salt tolerance at different developmental stages and increased ABA sensitivity, as indicated by delayed seed germination and stomatal closure. The GmSAP16 transgenic Arabidopsis plants had a higher proline content and a lower water loss rate and malondialdehyde (MDA) content than wild type (WT) plants in response to stresses. The overexpression of GmSAP16 in soybean hairy roots enhanced drought and salt tolerance of soybean seedlings, with higher proline and chlorophyll contents and a lower MDA content than WT. RNA inference (RNAi) of GmSAP16 increased stress sensitivity. Stress-related genes, including GmDREB1B;1, GmNCED3, GmRD22, GmDREB2, GmNHX1, and GmSOS1, showed significant expression alterations in GmSAP16-overexpressing and RNAi plants under stress treatments. These results indicate that soybean SAP genes play important roles in abiotic stress responses.

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“…Similar to humans, SAP genes in plants are involved in the pathogen-recognition process via membrane-bound receptor-like kinases [15], and in defence mechanisms against bacteria and fungi infections, as shown in the recent examples of tomato SlSAP3 and SlSAP4 silencing and over-expression [16,17]. However, much stronger responses of SAP genes were found in plants in response to various abiotic stresses including drought, salinity, cold and others (Reviewed in [2]).…”

Section: Introductionmentioning

confidence: 99%

Identification, gene expression and genetic polymorphism of zinc finger A20/AN1 stress-associated genes, HvSAP, in salt stressed barley from Kazakhstan

et al. 2020

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Background A family of genes designated as the Zinc finger A20/AN1 Transcription factors encoding stress-associated proteins (SAP) are well described in Arabidopsis and rice, and include 14 AtSAP and 18 OsSAP genes that are associated with variable tolerances to multiple abiotic stresses. The SAP gene family displays a great diversity in its structure and across different plant species. The aim of this study was to identify all HvSAP genes in barley (Hordeum vulgare L.), to analyse the expression of selected genes in response to salinity in barley leaves and develop SNP marker for HvSAP12 to evaluate the association between genotypes of barley plants and their grain yield in field trials. Results In our study, 17 HvSAP genes were identified in barley, which were strongly homologous to rice genes. Five genes, HvSAP5, HvSAP6, HvSAP11, HvSAP12 and HvSAP15, were found to be highly expressed in leaves of barley plants in response to salt stress in hydroponics compared to controls, using both semi-quantitative RT-PCR and qPCR analyses. The Amplifluor-like SNP marker KATU-B30 was developed and used for HvSAP12 genotyping. A strong association (R2 = 0.85) was found between KATU-B30 and grain yield production per plant of 50 F3 breeding lines originating from the cross Granal × Baisheshek in field trials with drought and low to moderate salinity in Northern and Central Kazakhstan. Conclusions A group of HvSAP genes, and HvSAP12 in particular, play an important role in the tolerance of barley plants to salinity and drought, and is associated with higher grain yield in field trials. Marker-assisted selection with SNP marker KATU-B30 can be applied in barley breeding to improve grain yield production under conditions of abiotic stress.

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Tomato SlSAP3, a member of the stress‐associated protein family, is a positive regulator of immunity against Pseudomonas syringae pv. tomato DC3000

Cited by 32 publications

References 49 publications

The root‐knot nematode effector MiPDI1 targets a stress‐associated protein (SAP) to establish disease in Solanaceae and Arabidopsis

The root‐knot nematode effector MiPDI1 targets a stress‐associated protein (SAP) to establish disease in Solanaceae and Arabidopsis

Genomic Analysis of Stress Associated Proteins in Soybean and the Role of GmSAP16 in Abiotic Stress Responses in Arabidopsis and Soybean

Identification, gene expression and genetic polymorphism of zinc finger A20/AN1 stress-associated genes, HvSAP, in salt stressed barley from Kazakhstan

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