Tomato responds to green peach aphid infestation with the activation of trehalose metabolism and starch accumulation

Singh, Vijay; Shah, Jyoti

doi:10.4161/psb.20066

Cited by 43 publications

(39 citation statements)

References 9 publications

(17 reference statements)

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“…In addition, enzymes involved in sucrose biosynthesis were also up-regulated. Previous studies suggested that sucrose biosynthesis was up-regulated during plant defense against pathogen and insects in a wide range of crop and model plant species, including rice, cotton, tomato, and Arabidopsis (47)(48)(49)(50)(51)(52)(53)(54). The study correlated with previous studies indicating the important roles for sucrose in plant defense against herbivorous insects.…”

Section: Parc As An Effective Platform For Shot-gun Proteomics-supporting

confidence: 84%

Application of an Improved Proteomics Method for Abundant Protein Cleanup: Molecular and Genomic Mechanisms Study in Plant Defense

Zhang

Gao

Xing

et al. 2013

Molecular & Cellular Proteomics

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High abundance proteins like ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco) impose a consistent challenge for the whole proteome characterization using shot-gun proteomics. To address this challenge, we developed and evaluated Polyethyleneimine Assisted Rubisco Cleanup (PARC) as a new method by combining both abundant protein removal and fractionation. The new approach was applied to a plant insect interaction study to validate the platform and investigate mechanisms for plant defense against herbivorous insects. Our results indicated that PARC can effectively remove Rubisco, improve the protein identification, and discover almost three times more differentially regulated proteins. The significantly enhanced shotgun proteomics performance was translated into in-depth proteomic and molecular mechanisms for plant insect interaction, where carbon re-distribution was used to play an essential role. Moreover, the transcriptomic validation also confirmed the reliability of PARC analysis. Finally, functional studies were carried out for two differentially regulated genes as revealed by PARC analysis. One of the constant challenges for proteomics is inadequate protein identification because of the interference of high abundance proteins (1). The challenge is particularly critical for plant proteomics analysis because of the prevalence of Rubisco (Ribulose-1,5-bisphosphate carboxylase oxygenase) in green tissue. As a major enzyme involved in carbon fixation, Rubisco consists of 30 to 50% of total plant protein from green tissues and causes less sensitivity, dynamic range, and protein identification of plant proteomics (2-4). Influences of high abundance proteins like Rubisco affect both gel-based and shot-gun proteomics analysis. In one of the most popular shot-gun proteomics platforms with the data-dependent MS/MS acquisition, the peptides derived from the abundant proteins have more chance to be sampled by the MS instrument than the peptides from other functional proteins. Thus, the dynamic range and detection sensitivity will be sacrificed because of the prevalence of high abundance proteins (2). To address this challenge, we developed and evaluated a new method by combining PEI (Polyethyleneimine) 1 precipitation and protein sample fractionation to improve the performance of multidimensional protein identification technology (Mud-PIT)-based proteomics analysis.PEI is a positively charged polymer broadly used for removing nucleic acids from proteins (5). The compound can also be employed to remove acidic proteins like Rubisco from the total protein (6). PEI precipitation can be considered as a fractionation process to separate acidic proteins from the total protein, and thus can be used for both Rubisco removal as well as fractionation of plant proteins from green tissues. Despite the potential to be used for sample preparation, very few studies optimized the PEI precipitation for plant proteomics analysis and evaluated the effectiveness of the approach for enhancing proteomics performance. In this study, w...

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Section: Parc As An Effective Platform For Shot-gun Proteomics-supporting

confidence: 84%

Application of an Improved Proteomics Method for Abundant Protein Cleanup: Molecular and Genomic Mechanisms Study in Plant Defense

Zhang

Gao

Xing

et al. 2013

Molecular & Cellular Proteomics

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“…GPA infestation also results in an increase in starch content in Arabidopsis leaves (Singh et al, 2011; Singh, 2012). This increase in starch was observed even when the plants were kept in complete darkness during the course of the experiment.…”

Section: The Arabidopsis Thaliana-green Peach Aphid Pathosystem: a Momentioning

confidence: 99%

“…This increase in starch was observed even when the plants were kept in complete darkness during the course of the experiment. Expression of the Arabidopsis APL3 gene, which encodes a subunit of AGPase that synthesizes ADP-glucose, the donor of glucosyl moieties to the growing starch chain (Geigenberger, 2011), was also upregulated in GPA-infested plants (Singh, 2012). In Arabidopsis the PGM1 gene encodes a phosphoglucomutase that synthesizes glucose-1-phosphate, which is the precursor for starch synthesis.…”

Section: The Arabidopsis Thaliana-green Peach Aphid Pathosystem: a Momentioning

confidence: 99%

Arabidopsis thaliana—Myzus persicae interaction: shaping the understanding of plant defense against phloem-feeding aphids

Louis¹,

Shah²

2013

Front. Plant Sci.

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The phloem provides a unique niche for several organisms. Aphids are a large group of Hemipteran insects that utilize stylets present in their mouthparts to pierce sieve elements and drink large volumes of phloem sap. In addition, many aphids also vector viral diseases. Myzus persicae, commonly known as the green peach aphid (GPA), is an important pest of a large variety of plants that includes Arabidopsis thaliana. This review summarizes recent studies that have exploited the compatible interaction between Arabidopsis and GPA to understand the molecular and physiological mechanisms utilized by plants to control aphid infestation, as well as genes and mechanisms that contribute to susceptibility. In addition, recent efforts to identify aphid-delivered elicitors of plant defenses and novel aphid salivary components that facilitate infestation are also discussed.

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“…As the concentration of trehalose in aphid honeydew is higher than what is normally found in plants, it may influence sugar metabolism in the leaves from which the aphids are feeding. Research involving both Arabidopsis and tomato genes suggested that TREHALOSE PHOSPHATE SYNTHASE11 activity regulates host defense during infestation with green peach aphid by providing a threshold level of signaling trehalose (Singh et al, 2011;Singh and Shah, 2012). However, whether plants regulate trehalose signaling through an internal pool or in response to exogenous addition from insect honeydew remains an open question (Lunn et al, 2014).…”

Section: Insect Secretionsmentioning

confidence: 99%

Alteration of plant primary metabolism in response to insect herbivory

et al. 2015

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ORCID IDs: 0000-0002-3643-7875 (S.Z.); 0000-0002-4716-4323 (Y.-R.L.); 0000-0002-5912-779X (V.T.); 0000-0002-9675-934X (G.J.).Plants in nature, which are continuously challenged by diverse insect herbivores, produce constitutive and inducible defenses to reduce insect damage and preserve their own fitness. In addition to inducing pathways that are directly responsible for the production of toxic and deterrent compounds, insect herbivory causes numerous changes in plant primary metabolism. Whereas the functions of defensive metabolites such as alkaloids, terpenes, and glucosinolates have been studied extensively, the fitness benefits of changes in photosynthesis, carbon transport, and nitrogen allocation remain less well understood. Adding to the complexity of the observed responses, the feeding habits of different insect herbivores can significantly influence the induced changes in plant primary metabolism. In this review, we summarize experimental data addressing the significance of insect feeding habits, as related to herbivore-induced changes in plant primary metabolism. Where possible, we link these physiological changes with current understanding of their underlying molecular mechanisms. Finally, we discuss the potential fitness benefits that host plants receive from altering their primary metabolism in response to insect herbivory.

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Tomato responds to green peach aphid infestation with the activation of trehalose metabolism and starch accumulation

Cited by 43 publications

References 9 publications

Application of an Improved Proteomics Method for Abundant Protein Cleanup: Molecular and Genomic Mechanisms Study in Plant Defense

Application of an Improved Proteomics Method for Abundant Protein Cleanup: Molecular and Genomic Mechanisms Study in Plant Defense

Arabidopsis thaliana—Myzus persicae interaction: shaping the understanding of plant defense against phloem-feeding aphids

Alteration of plant primary metabolism in response to insect herbivory

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