Methyl bromide has been banned worldwide because it causes damage to the ozone layer and the environment. To nd a substitute for methyl bromide, the relationships among fumigation, plant growth, and the microbial community in replant soil require further study. We performed pot and eld experiments to investigate the effects of dazomet fumigation on soil properties and plant performance. Changes in soil microbial community structure and diversity were assessed using high-throughput sequencing, and plant physiological performance and soil physicochemical properties were also measured. Dazomet fumigation enhanced photosynthesis and promoted plant growth in replant soil; it altered soil physical and chemical properties and reduced soil enzyme activities, although these parameters gradually recovered over time. After dazomet fumigation, the dominant soil phyla changed, microbial diversity decreased signi cantly, the relative abundance of biocontrol bacteria such as Mortierella increased, and the relative abundance of pathogenic bacteria such as Fusarium decreased. Over the course of the experiment, the soil microbial ora changed dynamically, and soil enzyme activities and other physical and chemical properties also recovered to a certain extent. This result suggested that the effect of dazomet on soil microorganisms was temporary. However, fumigation also led to an increase in some resistant pathogens, such as Trichosporon, that affect soil function and health. Therefore, it is necessary to consider potential negative impacts of dazomet on the soil environment and to perform active environmental risk management in China.
HighlightsFumigation of apple replant soil with dazomet renewed the soil microbial environment, improved soil physical and chemical properties, and dynamically restored soil ecological function.The activities of several soil enzymes and the relative abundance of bene cial microorganisms increased after dazomet fumigation. These changes were accompanied by higher rates of apple photosynthesis and growth.Fumigation reduced the content of pathogenic fungi such as Fusarium and increased the abundance of bene cial bacteria in the soil. However, it also increased the abundance of pathogenic bacteria such as Trichosporon, indicating that it is important to perform active environmental risk management.