Tomato Fruit Acid Invertase Complementary DNA : Nucleotide and Deduced Amino Acid Sequences

Klann, Ellen; Yelle, Serge; Bennett, A. B.

doi:10.1104/pp.99.1.351

Cited by 58 publications

(9 citation statements)

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“…Degenerated primers were constructed (Pharmacia Gene-Assembler) to obtain polymerase chain reaction (PCR) probes in order to isolate invertase and sucrose synthase cDNA clones from the library. Invertase primer design was based on sequences of carrot (Sturm and Chrispeels 1990) and tomato (Klann et al 1992) cDNA clones. The sequence of the forward primer was 5¢AAYTGGATNAAYGAYCCNAAYGG3¢ and that of the reverse primer 5¢AARTCNNNRCAYTGCCACATNCC3¢.…”

Section: Methodsmentioning

confidence: 99%

Rapid stalk elongation in tulip ( Tulipa gesneriana L. cv. Apeldoorn) and the combined action of cold-induced invertase and the water-channel protein γTIP

Балк

Boer

1999

Planta

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Many bulbous plants need a low-temperature treatment for flowering. Cold, for example, affects the elongation of the stalk, thereby influencing the quality of the cut flower. How the elongation of the stalk is promoted by cold and which physiological and biochemical mechanisms are involved have remained obscure. As invertase has been shown to be involved in the cold-induced elongation of the flower stalks of tulips (Lambrechts et al., 1994, Plant Physiol 104: 515-520), we further characterized this enzyme by cloning the cDNA and analysing its expression in various tissues of the tulip (Tulipa gesneriana L. cv. Apeldoorn) stalk. In addition, the role of sucrose synthase was investigated. Since turgor pressure is an important force driving cell elongation, the role of a water-channel protein (gammaTIP) was studied in relation to these two enzymes. The mRNA level of the invertase found was substantially up-regulated as a result of cold treatment. Analysis of the amino acid sequence of this invertase revealed the presence of a vacuolar targeting signal. Two different forms of sucrose synthase were found, the expression of one of them appeared to be restricted to the vascular tissue while the other form was present in the surrounding tissue. Both sucrose synthases were present in the stalk during the entire period of bulb storage and after planting, but their activities declined during stalk elongation. The expression of the gammaTIP gene was restricted mainly to the vascular tissue and its expression profile was identical to that of invertase. Simultaneous expression of invertase and gammaTIP possibly leads to an increase in osmotic potential and vacuolar water uptake, thus providing a driving force for stretching the stalk cells.

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Section: Methodsmentioning

confidence: 99%

Rapid stalk elongation in tulip ( Tulipa gesneriana L. cv. Apeldoorn) and the combined action of cold-induced invertase and the water-channel protein γTIP

Балк

Boer

1999

Planta

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“…cDNAs and genomic DNAs for soluble and cell wallbound acid invertases have been isolated from various plants (Sturm and Chrispeels, 1990;Arai et al, 1992;Klann et al, 1992;Elliott et al, 1993;Unger et al, 1994;Roitsch et al, 1995;Weber et al, 1995). In tomato (Lycopersicon esculentum), in efforts to elucidate the mechanisms responsible for the accumulation of sugars in fruit, genes for soluble acid invertase have been studied intensively (Klann et al, 1992(Klann et al, , 1996Ohyama et al, 1992Ohyama et al, , 1995Chetelat et al, 1993Chetelat et al, , 1995Elliott et al, 1993;Sato et al, 1993;Hadas et al, 1995), while those for cell wall-bound acid invertase(s) have not received so much attention (Godt and Roitsch, 1997). We report here the cloning of a cDNA for a cell wall-bound acid invertase from wounded source leaves of tomato, as well as a comparison of the expression of isozymes of soluble and cell wall-bound invertases in plants and wounded leaves of L. esculentum and a wild relative, L. peruvianum.…”

Section: Introductionmentioning

confidence: 99%

Cloning of cDNA for a cell wall-bound acid invertase from tomato (Lycopersicon esculentum) and expression of soluble and cell wall-bound invertases in plants and wounded leaves of L. esculentum and L. peruvianum.

Ohyama¹,

Nishimura²,

Hirai³

1998

Genes Genet. Syst.

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A cDNA, Wiv-1, for an isozyme of acid invertase (EC 3.2.1.26) was cloned from wounded leaves of tomato (Lycopersicon esculentum). The encoded protein had a basic isoelectric point and strong similarity to the amino acid sequences of plant cell wall-bound invertases. The conserved sequence WECPD that is found in all plant cell wall-bound invertases was also found in the deduced protein. These results suggested that Wiv-1 encoded a cell wall-bound acid invertase of tomato. Wounding increased the levels of mRNAs for soluble and cell wall-bound invertases and the activities of these invertases in leaves of L. esculentum and of a related species, L. peruvianum. The induction of Aiv-1 mRNA for the soluble enzyme in wounded leaves was not very strong, while that of Wiv-1 mRNA for the wall-bound enzyme was prominent. The level of Aiv-1 mRNA reached a maximum 48 h after wounding while that of Wiv-1 mRNA continued to rise for up to 96 h. These findings suggested that the genes for the two isozymes responded independently to wounding. The levels in various organs of Aiv-1 and Wiv-1 mRNAs were higher in L. esculentum than in L. peruvianum. Possible roles of cell wall-bound acid invertase in wound response and in developing plant are discussed.

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“…The corresponding values for the carrot (EMBL accession number X67163), tomato [9] and mung bean [1] vacuolar enzymes are 44~o, 4 3~ and 46~o identity (62~o, 61~o and 63 ~o similarity) respectively. Throughout the sequence there are several regions which are highly conserved in plant and other invertases, including sequence around the putative active site cysteine residue (Fig.…”

Section: Atatc-g Tgaatattg T T a T A~ 1949mentioning

confidence: 99%

“…Enzymes with this activity have been purified and characterised from a wide variety of plants and plant tissues [2,3,4,10,12,20,21]. The acidic invertases (with activity optima in the range pH 3.5-5.1) are thought to function in the vacuole of the plant cell and in the apoplast, while alkaline invertases (pH 7.0-7.8 optimum) are cytoplasmic, cDNAs corresponding to the mRNAs encoding both extracellular and vacuolar invertases have been cloned from carrot ( [ 18] and EMBL accession number X67163 respectively) and for a vacuolar invertase from tomato [9]. A cDNA for the mRNA encoding two forms ofmung bean invertase has also been characterised [1].…”

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confidence: 99%

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cDNA cloning and expression of a potato (Solanum tuberosum) invertase

Hedley¹,

Machray²,

Davies³

et al. 1993

Plant Mol Biol

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A cDNA clone encoding an invertase isoenzyme has been isolated from a potato leaf cDNA library. The deduced amino acid sequence shows significant similarities to previously characterised invertases. The highest degree of overall similarity, including the signal peptide sequence, is to carrot cell wall invertase, suggesting that the potato gene encodes an apoplastic enzyme. Expression of the gene, as determined by RT-PCR, is detected in stem and leaf tissue, and at lower levels in tuber, but is absent from roots.

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Tomato Fruit Acid Invertase Complementary DNA : Nucleotide and Deduced Amino Acid Sequences

Cited by 58 publications

References 0 publications

Rapid stalk elongation in tulip ( Tulipa gesneriana L. cv. Apeldoorn) and the combined action of cold-induced invertase and the water-channel protein γTIP

Rapid stalk elongation in tulip ( Tulipa gesneriana L. cv. Apeldoorn) and the combined action of cold-induced invertase and the water-channel protein γTIP

Cloning of cDNA for a cell wall-bound acid invertase from tomato (Lycopersicon esculentum) and expression of soluble and cell wall-bound invertases in plants and wounded leaves of L. esculentum and L. peruvianum.

cDNA cloning and expression of a potato (Solanum tuberosum) invertase

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