Tomato facultative parthenocarpy results from Sl<i><scp>AGAMOUS</scp>‐<scp>LIKE</scp>6</i>loss of function

Klap, Chen; Yeshayahou, Ester; Bolger, Anthony; Arazi, Tzahi; Gupta, Suresh; Shabtai, Sara; Usadel, Björn; Salts, Yehiam; Barg, Rivka

doi:10.1111/pbi.12662

Cited by 269 publications

(155 citation statements)

References 72 publications

(99 reference statements)

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“…Taking an even more sophisticated approach in tomato, Rodríguez‐Leal et al () demonstrated that a whole range of variability in locule number and fruit size can be obtained by producing an array of CLV3 promoter variants with different fragment deletions. Cas9‐mediated knockout of AGL6 caused facultative parthenocarpy in tomato, which results in seedless fruits (Klap et al ). Reduced seed shattering was achieved by knocking out the ALCATRAZ gene of rapeseed (Braatz et al ).…”

Section: Applications Of Targeted Genome Modification By Nhejmentioning

confidence: 99%

The CRISPR/Cas revolution continues: From efficient gene editing for crop breeding to plant synthetic biology

Kumlehn

Pietralla

Hensel

et al. 2018

JIPB

100

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Since the discovery that nucleases of the bacterial CRISPR (clustered regularly interspaced palindromic repeat)‐associated (Cas) system can be used as easily programmable tools for genome engineering, their application massively transformed different areas of plant biology. In this review, we assess the current state of their use for crop breeding to incorporate attractive new agronomical traits into specific cultivars of various crop plants. This can be achieved by the use of Cas9/12 nucleases for double‐strand break induction, resulting in mutations by non‐homologous recombination. Strategies for performing such experiments − from the design of guide RNA to the use of different transformation technologies − are evaluated. Furthermore, we sum up recent developments regarding the use of nuclease‐deficient Cas9/12 proteins, as DNA‐binding moieties for targeting different kinds of enzyme activities to specific sites within the genome. Progress in base deamination, transcriptional induction and transcriptional repression, as well as in imaging in plants, is also discussed. As different Cas9/12 enzymes are at hand, the simultaneous application of various enzyme activities, to multiple genomic sites, is now in reach to redirect plant metabolism in a multifunctional manner and pave the way for a new level of plant synthetic biology.

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Section: Applications Of Targeted Genome Modification By Nhejmentioning

confidence: 99%

The CRISPR/Cas revolution continues: From efficient gene editing for crop breeding to plant synthetic biology

Kumlehn

Pietralla

Hensel

et al. 2018

JIPB

100

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“…Regarding MBS, because of the comparatively low number of EMS‐induced SNPs, reducing the candidate genetic variants to few SNPs or possibly to a single SNP when present in a highly recombigenic region, can be straightforward. Reported sizes for MBS‐mapped traits are typically in the 1–4 Mb range and the number of candidate mutations is <10, as indicated for the fruit color mutant psy1, cutin deficient mutant gpat6‐a and fruit parthenocarpy mutation in SlAGL6 gene (Garcia et al ., ; Petit et al ., ; Klap et al ., ). Recombinant analysis is then usually performed to unequivocally identify the causal mutation (Figure ).…”

Section: Accelerating Trait Discovery In Tomato Using Deep Sequencingmentioning

confidence: 97%

Trait discovery and editing in tomato

2018

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Summary Tomato (Solanum lycopersicum), which is used for both processing and fresh markets, is a major crop species that is the top ranked vegetable produced over the world. Tomato is also a model species for research in genetics, fruit development and disease resistance. Genetic resources available in public repositories comprise the 12 wild related species and thousands of landraces, modern cultivars and mutants. In addition, high quality genome sequences are available for cultivated tomato and for several wild relatives, hundreds of accessions have been sequenced, and databases gathering sequence data together with genetic and phenotypic data are accessible to the tomato community. Major breeding goals are productivity, resistance to biotic and abiotic stresses, and fruit sensorial and nutritional quality. New traits, including resistance to various biotic and abiotic stresses and root architecture, are increasingly being studied. Several major mutations and quantitative trait loci (QTLs) underlying traits of interest in tomato have been uncovered to date and, thanks to new populations and advances in sequencing technologies, the pace of trait discovery has considerably accelerated. In recent years, clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing (GE) already proved its remarkable efficiency in tomato for engineering favorable alleles and for creating new genetic diversity by gene disruption, gene replacement, and precise base editing. Here, we provide insight into the major tomato traits and underlying causal genetic variations discovered so far and review the existing genetic resources and most recent strategies for trait discovery in tomato. Furthermore, we explore the opportunities offered by CRISPR/Cas9 and their exploitation for trait editing in tomato.

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“…In contrast, to date, only two advanced studies in tomato have been described: precise targeting of the pectate lyase ( PL ) gene, which resulted in delayed fruit softening without perturbing other ripening-related parameters (Uluisik et al, 2016); and editing the SlAGAMOUS-LIKE 6 ( SlAGL6 ), a MADS-box transcription factor which provides tolerance to heat stress conditions and results in parthenocarpic fruits (Klap et al, 2016). …”

Section: New Plant Breeding Techniques (Npbt) For Fruit Quality Studiesmentioning

confidence: 99%

Use of Natural Diversity and Biotechnology to Increase the Quality and Nutritional Content of Tomato and Grape

Gascuel

Diretto²,

Monforte

et al. 2017

Front. Plant Sci.

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Improving fruit quality has become a major goal in plant breeding. Direct approaches to tackling fruit quality traits specifically linked to consumer preferences and environmental friendliness, such as improved flavor, nutraceutical compounds, and sustainability, have slowly been added to a breeder priority list that already includes traits like productivity, efficiency, and, especially, pest and disease control. Breeders already use molecular genetic tools to improve fruit quality although most advances have been made in producer and industrial quality standards. Furthermore, progress has largely been limited to simple agronomic traits easy-to-observe, whereas the vast majority of quality attributes, specifically those relating to flavor and nutrition, are complex and have mostly been neglected. Fortunately, wild germplasm, which is used for resistance against/tolerance of environmental stresses (including pathogens), is still available and harbors significant genetic variation for taste and health-promoting traits. Similarly, heirloom/traditional varieties could be used to identify which genes contribute to flavor and health quality and, at the same time, serve as a good source of the best alleles for organoleptic quality improvement. Grape (Vitis vinifera L.) and tomato (Solanum lycopersicum L.) produce fleshy, berry-type fruits, among the most consumed in the world. Both have undergone important domestication and selection processes, that have dramatically reduced their genetic variability, and strongly standardized fruit traits. Moreover, more and more consumers are asking for sustainable production, incompatible with the wide range of chemical inputs. In the present paper, we review the genetic resources available to tomato/grape breeders, and the recent technological progresses that facilitate the identification of genes/alleles of interest within the natural or generated variability gene pool. These technologies include omics, high-throughput phenotyping/phenomics, and biotech approaches. Our review also covers a range of technologies used to transfer to tomato and grape those alleles considered of interest for fruit quality. These include traditional breeding, TILLING (Targeting Induced Local Lesions in Genomes), genetic engineering, or NPBT (New Plant Breeding Technologies). Altogether, the combined exploitation of genetic variability and innovative biotechnological tools may facilitate breeders to improve fruit quality tacking more into account the consumer standards and the needs to move forward into more sustainable farming practices.

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Tomato facultative parthenocarpy results from SlAGAMOUS‐LIKE6loss of function

Cited by 269 publications

References 72 publications

The CRISPR/Cas revolution continues: From efficient gene editing for crop breeding to plant synthetic biology

The CRISPR/Cas revolution continues: From efficient gene editing for crop breeding to plant synthetic biology

Trait discovery and editing in tomato

Use of Natural Diversity and Biotechnology to Increase the Quality and Nutritional Content of Tomato and Grape

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