Tomatidine inhibits iNOS and COX‐2 through suppression of NF‐κB and JNK pathways in LPS‐stimulated mouse macrophages

Chiu, Feng‐Lan; Lin, Jen‐Kun

doi:10.1016/j.febslet.2008.05.049

Cited by 120 publications

(100 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previous studies demonstrated a-tomatine has exhibited antiproliferative and apoptotic effects on the growth of cancer cells originating from the human colon and liver [22]. In addition, a-tomatine also induced T-cell-mediated regression of murine lymphoid experimental tumors, EG7-Ova [23], and acted as an anti-inflammatory agent by blocking NF-jB and JNK signaling in mouse macrophages [24]. In this study, we employed human non-small cell lung cancer NCI-H460 cells to assess the molecular mechanism responsible for the antimetastatic effect of a-tomatine.…”

Section: Introductionmentioning

confidence: 96%

α-Tomatine Suppresses Invasion and Migration of Human Non-Small Cell Lung Cancer NCI-H460 Cells Through Inactivating FAK/PI3K/Akt Signaling Pathway and Reducing Binding Activity of NF-κB

Shieh

Cheng

Shi

et al. 2011

Cell Biochem Biophys

View full text Add to dashboard Cite

α-Tomatine, isolated from Lycopersicon esculentum Linn., is a naturally occurring steroidal glycoalkaloid in immature green tomatoes. Some reports demonstrated that α-tomatine had various anticarcinogenic properties. The purpose of this study is to investigate the anti-metastatic effect of α-tomatine in NCI-H460 human non-small cell lung cancer cells. First, the results showed that α-tomatine significantly suppressed the abilities of the adhesion, invasion, and migration of NCI-H460 cells under non-cytotoxic concentrations. Molecular data also showed α-tomatine could inhibit the activation of focal adhesion kinase (FAK) and phosphatidylinositol 3-kinase (PI3K)/Akt signal involve in the downregulation the enzyme activities, protein and messenger RNA levels of matrix metalloproteinase-7 (MMP-7). Next, α-tomatine also strongly inhibited the degradation of inhibitor of kappaBα (IκBα) and the nuclear levels of nuclear factor kappa B (NF-κB). Also, a dose-dependent inhibition on the binding ability of NF-κB by α-tomatine treatment was further observed. Furthermore, α-tomatine significantly decreased the levels of phospho-Akt and MMP-7 in Akt1-cDNA-transfected cells concomitantly with a marked reduction on cell invasion and migration. Presented results indicated α-tomatine might be further application for treating cancer metastasis.

show abstract

Section: Introductionmentioning

confidence: 96%

α-Tomatine Suppresses Invasion and Migration of Human Non-Small Cell Lung Cancer NCI-H460 Cells Through Inactivating FAK/PI3K/Akt Signaling Pathway and Reducing Binding Activity of NF-κB

Shieh

Cheng

Shi

et al. 2011

Cell Biochem Biophys

View full text Add to dashboard Cite

show abstract

“…On evaluating the mechanistic effect of α-tomatine, it has been proven that tomatine alters essential signaling pathways involved in the maintenance of cell proliferation, migration and differentiation (13,14). Inhibition in the growth of human liver cancer HepG2 and human colon cancer HT29 cell lines was observed following in vitro α-tomatine treatment, and the results were found to be more effective compared with those of standard anticancer drugs, such as camptothecin and doxorubicin (15,16).…”

Section: Introductionmentioning

confidence: 87%

Molecular mechanisms underlying the α‑tomatine‑directed apoptosis in human malignant glioblastoma cell lines A172 and U‑118 MG

2017

View full text Add to dashboard Cite

Abstract. In the present study, the molecular mechanisms involved in the α-tomatine-induced apoptosis in human glioblastoma cell lines A172 and U-118 MG were investigated. Wright staining and ApopTag assays were conducted to confirm the apoptosis induced by α-tomatine treatment. Fura-2 assay determined an enhancement in free Ca 2+ intracellularly, indicating the occurrence of Ca 2+ -dependent apoptosis induction. Western blot experiments were also performed to predict the apoptosis by measuring the changes in the Bax:Bcl-2 ratio. Increase of calpain activity triggered caspase-12 expression, which in turn further activated caspase-9. In addition, an increase in the ratio of Bax:Bcl-2 accounted for the mitochondrial release of cytochrome c into the cytosol for caspase-3 and caspase-9 activation. Elevated activity of calpain and caspase-3 yielded spectrin breakdown products with 145 and 120 kDa, respectively. Caspase-3 activation further cleaved the inhibitor of caspase activated DNase, while the apoptosis-inducing factor detected in the cytosol suggested that apoptosis was independent of caspase. The apoptosis induction was further supported by decreased expression levels of nuclear factor-κB and increased expression of the inhibitor of nuclear factor, IκBα. In conclusion, the presented experimental results revealed the stimulation of different molecular mechanisms for α-tomatine-mediated apoptosis in A172 and U-118 MG human glioblastoma cell lines.

show abstract

“…It has now accepted that inflammation plays a crucial role in chronic inflammatory diseases and the cancer development [5,6]. Inflammatory leukocytes, such as macrophages when activated by many stimuli, result in the induction of pro-inflammatory protein and enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) [7]. Consequently, suppressing the induction of COX-2 and iNOS is a key strategy for cancer chemoprevention in various human organs [8].…”

Section: Introductionmentioning

confidence: 99%

Some Phytochemicals and Anti-inflammation Effect of Juice from <i>Tiliacora triandra</i> Leaves

Weerawatanakorn¹,

Rojsuntornkitti²,

Pan³

et al. 2018

JFNR

View full text Add to dashboard Cite

Tiliacora triandra (TT), native to Southeast Asia, has been widely used as folk medicine and in cuisines in many areas. Although little scientific evidence supports the health benefits of TT leaves, the juice extracted from TT leaves has become popular for consumers among all socioeconomic classes. Thus, this study aims to evaluate the phytochemical profile and anti-inflammatory properties of juice extracted from TT leaves. The result revealed that, the highest total phenolic contents (199.92 mg GAE/g), total flavonoid contents (29.76 mg RUE/g), and extraction yield (61.2%) of the lyophilized TT leaves juice powder were found in the solvent mixture of ethanol, water, and acetone extracts. The highest DPPH radical scavenging value (90.95%) of the lyophilized TT leaves juice powder was found in hot water extract. By HPLC analysis, total phenolic compounds of the lyophilized TT leaves juice powder was 3,938.1 mg/kg. Tannic acid, gallic acid, and rutin are the major phenolic compound and the juice is a rich source of chlorophyll compound (3,551.6 mg/kg). The lyophilized TT leaves juice down-regulated the induction of inflammatory iNOS and COX-2 proteins in LPS-stimulated macrophages. The results suggest that intake of TT leaves juice, providing various phenolic and chlorophyll compounds, has great potential for reducing the inflammatory process.

show abstract

Tomatidine inhibits iNOS and COX‐2 through suppression of NF‐κB and JNK pathways in LPS‐stimulated mouse macrophages

Cited by 120 publications

References 38 publications

α-Tomatine Suppresses Invasion and Migration of Human Non-Small Cell Lung Cancer NCI-H460 Cells Through Inactivating FAK/PI3K/Akt Signaling Pathway and Reducing Binding Activity of NF-κB

α-Tomatine Suppresses Invasion and Migration of Human Non-Small Cell Lung Cancer NCI-H460 Cells Through Inactivating FAK/PI3K/Akt Signaling Pathway and Reducing Binding Activity of NF-κB

Molecular mechanisms underlying the α‑tomatine‑directed apoptosis in human malignant glioblastoma cell lines A172 and U‑118 MG

Some Phytochemicals and Anti-inflammation Effect of Juice from <i>Tiliacora triandra</i> Leaves

Contact Info

Product

Resources

About