Toluidine blue staining of murine mast cells and quantitation by a novel, automated image analysis method using whole slide skin images

Raja, Nivethitha; Naikodi, Sangmesh B; Govindarajan, Arunprasath; Palanisamy, Kamalavenkatesh

doi:10.1080/01478885.2021.1915934

Cited by 10 publications

(4 citation statements)

References 6 publications

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“…In line with these findings, other investigators have also successfully employed Romanowsky-type labeling for mast cells. [22][23][24][25] There seems to be agreement between the current study and others 2,27 which suggest that IHC is a more sensitive method of labeling mast cells as compared with that of the Wright stain. One last limitation of this study is the use of CD117 and tryptase as markers for mast cells.…”

Section: Discussionsupporting

confidence: 86%

“…Although variations of toluidine blue have been used successfully for mast cell identification, 16–22 the investigators in the current study were unable to achieve results superior to Wright with various toluidine blue protocols. In line with these findings, other investigators have also successfully employed Romanowsky-type labeling for mast cells 22–25 . There seems to be agreement between the current study and others 2,27 which suggest that IHC is a more sensitive method of labeling mast cells as compared with that of the Wright stain.…”

Section: Discussionmentioning

confidence: 67%

“…A further limitation of this study may be the choice of Wright histochemical stain for mast cells. Although variations of toluidine blue have been used successfully for mast cell identification, [16][17][18][19][20][21][22] the investigators in the current study were unable to achieve results superior to Wright with various toluidine blue protocols. In line with these findings, other investigators have also successfully employed Romanowsky-type labeling for mast cells.…”

Section: Discussionmentioning

confidence: 94%

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Mast Cell Concentrations in Pancreatic Disease Processes

Wynn,

Hodges,

Criswell

2023

Applied Immunohistochemistry &Amp; Molecular Morphology

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Mast cells enumeration has been performed using various histologic staining techniques with the goal of elucidating the influence mast cells exert on pathologic processes. In this study, 77 human pancreatic tissues evidencing morphologically normal pancreas, benign fibrotic changes, endocrine tumors, and adenocarcinoma were evaluated using Wright stain and immunohistochemistry markers for tryptase and CD117. Mast cell counts were similar with tryptase and CD117 but were both significantly higher than counts obtained with the Wright stain. Furthermore, all analyses demonstrated that endocrine tumors and morphologically normal pancreatic tissues had significantly lower mast cell counts as compared with benign fibrosis and adenocarcinoma suggesting that the highly fibrotic nature of both pancreatitis and adenocarcinoma are related to increased mast cell concentrations.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 94%

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Mast Cell Concentrations in Pancreatic Disease Processes

Wynn,

Hodges,

Criswell

2023

Applied Immunohistochemistry &Amp; Molecular Morphology

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“…These cells showed a positive reaction to different types of neutral (magenta) and acid (blue) mucopolysaccharides (Alesci et al, 2015, Figure 2c). Histochemical characterization with Toluidine Blue, a typical staining for MCs (Raja et al, 2021), highlighted groups of MCs with metachromatic cytoplasmic granules (Hrckova et al, 2006, Figure 2d).…”

Section: Resultsmentioning

confidence: 99%

Mast cells in goldfish (Carassius auratus) gut: Immunohistochemical characterization

et al. 2022

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The common goldfish is the most widespread teleosts in the world. Due to its peculiar characteristics, such as the high resistance, easy availability and stabulation, and for its evolutionary characteristics, this fish lends itself to be one of the most used experimental models. This study aimed to characterize the mast cells in the intestine of Carassius auratus using anti‐TLR‐2, anti‐S100, anti‐VIP, anti‐serotonin (5‐HT) and anti‐Piscidin antibodies. The intestine of goldfish, like that of all vertebrates, plays an important role in the immunology of the animal. The gut‐associated lymphoid tissue GALT is an immune component containing several specific cells such as lymphocytes, macrophages and mast cells. In addition, the presence of goblet cells in the intestinal epithelium strengthens the defence system, secreting many cytokines and chemokines and displaying antibacterial properties. Our results show mast cells labelled with antibodies that are highly conserved between fish and mammals, demonstrating an active role of these cells in the immune response.

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