Toluene inhalation induced epididymal sperm dysfunction in rats

Ono, Atsushi; Kawashima, Kunio; Sekita, Kiyoshi; Hirose, Akihiko; Ogawa, Yukio; Saito, Minoru; Naito, Katsushi; Yasuhara, Kazuo; Kaneko, Toyozo; Furuya, Tsuyoshi; Inoue, Tohru; Kurokawa, Yuji

doi:10.1016/s0300-483x(99)00120-1

Cited by 19 publications

(9 citation statements)

References 28 publications

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“…In addition to decreased spleen weight, thymus weight, and lymphocyte counts, the higher concentration lead to significantly reduced epididymal sperm counts, sperm motility, sperm quality, and sperm penetration in an in vitro test and to increased rates of abnormal sperm morphology. This toluene exposure did not significantly affect testes size, spermatogenesis, or serum levels of LH, FSH, or testosterone [Ono et al 1999]. Curiously, a 2-month daily exposure to 1,000 ppm toluene was found to protect Sprague-Dawley rats from the severe testicular atrophy and permanent testicular damage produced by similar simultaneous n-hexane inhalation [Nylen et al 1989].…”

Section: Impact Of Toluene On Fertility and Reproductive Functionmentioning

confidence: 85%

“…Inhalation exposures that begin to approach the levels seen in toluene abuse can have deleterious consequences on male reproductive function. In one study, young adult male SpragueDawley rats were exposed to 4,000 or 6,000 ppm toluene by inhalation 2 h per day for one month [Ono et al 1999]. In addition to decreased spleen weight, thymus weight, and lymphocyte counts, the higher concentration lead to significantly reduced epididymal sperm counts, sperm motility, sperm quality, and sperm penetration in an in vitro test and to increased rates of abnormal sperm morphology.…”

Section: Impact Of Toluene On Fertility and Reproductive Functionmentioning

confidence: 99%

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Reproductive Toxicology and Teratology of Abused Toluene

Hannigan

Bowen

2010

Systems Biology in Reproductive Medicine

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Reproductive Toxicology and Teratology of Abused TolueneToluene is an organic solvent that is widely used by industry and is ubiquitous in our environment. As a result, exposure to solvents like toluene in workrelated settings (i.e., relatively constant, low-level exposures) or through inhalant abuse (i.e., relatively intermittent, high-level exposures) is increasing for many women of reproductive age. Evidence suggests that the risk for pregnancy problems, as well as developmental delays and neurobehavioral difficulties, is higher for the children of women who have been exposed to high concentrations of organic solvents during pregnancy than for those who have not. These risks appear to be higher in cases of abuse exposure to solvents such as toluene, particularly in comparison to the risk for teratogenic outcomes with occupational solvent exposure. Despite this, the reproductive toxicology and teratology following abuse of toluene and other inhalants remains under-investigated. This brief review describes the current state of our understanding of the reproductive and teratogenic risk of gestational toluene abuse. The data to date suggest that the high levels of toluene exposure typical with inhalant abuse are more detrimental to fetal development than typical occupational exposure, and preclinical paradigms can be beneficial for investigating the processes and risks of prenatal solvent exposure. While substantial research has been done on the reproductive effects of occupational exposures to organic solvents, more research is needed on the outcomes and mechanisms of exposures typical of inhalant abuse.

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Section: Impact Of Toluene On Fertility and Reproductive Functionmentioning

confidence: 85%

Section: Impact Of Toluene On Fertility and Reproductive Functionmentioning

confidence: 99%

Reproductive Toxicology and Teratology of Abused Toluene

Hannigan

Bowen

2010

Systems Biology in Reproductive Medicine

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“…Evidence about toluene’s effect on testosterone serum levels is inconclusive. According to one study, testosterone did not change after 7 or 28 days of exposure (2 hr/day) to 6,000 ppm toluene in adolescent rats (Ono et al., ). In contrast, injection of 500 mg/kg toluene (subcutaneously, once a day for 10 days) was reported to significantly decrease testosterone levels in young adult rats (Nakai et al., ).…”

Section: Discussionmentioning

confidence: 97%

Sexual behaviour is impaired by the abused inhalant toluene in adolescent male rats

Violante-Soria

Cruz

Rodrı́guez–Manzo

2018

Eur J of Neuroscience

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Inhalant misuse is a worldwide problem, especially among adolescents. Toluene is the most widely misused inhalant. One hallmark of adolescence is the emergence of sexual behaviour, which can be affected by drug use. The aim of this study was to analyse the effects of toluene inhalation on different aspects of male rats' sexual behaviour using a binge pattern of exposure in adolescent rats. Male Wistar rats were individually exposed to air or 6,000 ppm toluene for 30 min (acute exposure; n = 8 each) or twice a day for 12 days (repeated exposure; n = 9 each) in static exposure chambers. Independent groups of sexually experienced, adolescent rats (postnatal day 63, PN63) were tested after acute toluene exposure for copulation, sexual incentive motivation or noncontact erections. Sexually naïve, adolescent rats (PN45-59) were repeatedly exposed to toluene and tested for sexual behaviour after completing the 1st, 3rd, 5th, 7th, 9th, 11th and 13th exposure sessions. Acute toluene exposure impaired copulatory performance, diminished sexual incentive motivation and delayed noncontact erection occurrence in sexually experienced rats. Repeated toluene exposure during adolescence completely inhibited the onset of copulatory behaviours in sexually naïve rats, at the time at which they should have appeared. However, once the inhalant exposure ended, copulatory responses appeared gradually, and animals attained a typical, stable copulatory pattern. In conclusion, acute toluene exposure impairs sexual behaviour in young, sexually experienced animals, while repeated toluene exposure during adolescence prevents the onset of copulatory behaviour, although this effect is transitory.

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“…18 Meanwhile, research by Lemasters et alstates that xylene and toluene exposure of >50 ppm for 30 weeks in painters has influenced the spermatogenesis process. 11 The results of the research conducted by the authors showed that toluene exposure had influenced MDA levels. Meanwhile, the researches of Lemasters et al and Ono et al with new measures of high doses affected MDA levels.…”

Section: Discussionmentioning

confidence: 98%

“…5 Meanwhile, other researches show that toluene exposure for 20 days with a dose of 1500 ppm for 4 hours per day against male Wistar rats did not directly affect the spermatogenesis process. 11 This research aims to find out the effect of toluene exposure on the early phase of spermatogenesis, namely Spermatogenia A. If any disruption occurs on Spermatogenia A, then the spermatogenesis process will also be affected.…”

Section: Toluenementioning

confidence: 99%

The Effect of Exposure Process of Toluene To The Spermatogonia Cell a Rattus Strain Wistar

et al. 2021

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Intoduction: Acute and chronic exposure to toluene at high doses is known to affect all organs of the body including the spermatogenesis process. In the industrial sector, the use of toluene as a solvent is still widely used, up to 10 million tons per year. The control over health problems that may occur is carried out by applying work exposure threshold values. This research aims to explore the effect of toluene exposure at the threshold value range on spermatogenesis.Method: This research used laboratory experiment on 30 male Wistar rats which were divided into five groups of different exposure levels, namely 12.5 parts per million (ppm], 25 ppm, 50 ppm, 100 ppm, and no exposure (control). Exposure was given for 4 hours daily over 14 days through a hood with measured release in the glass cage. The toluene exposure markers observed were Malondialdehyde (MDA) in the blood tissue and testicles using the Thiobarbituric Acid Reactive Substances (TBARS) method. The effect on the spermatogenicity process was assessed by counting the spermatogonia A cells of male Wistar rats with Periodic Acid Schiff (PAS) staining and is calculated by the Abercrombie formula. Analysis of the correlation between the level of exposure and its effect on the increase in malondialdehyde, and spermatogenesis was carried out using the Spearman correlation analysis.Result: There was a moderately positive correlation between levels of toluene exposure and plasma MDA levels (r = 0.42; p = 0.025). Meanwhile, on [the issue of] the quantity of spermatogonia cells, a high level of negative correlation with exposure levels was obtained (r = -0.68; p = 0.001).Conclusion: Toluene exposure in male Wistar rats within the range of threshold values influenced the increase in plasma MDA levels and decreased the Spermatogenia A cells. However, toluene exposure did not affect the testicular MDA levels of male Wistar rats.

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Toluene inhalation induced epididymal sperm dysfunction in rats

Cited by 19 publications

References 28 publications

Reproductive Toxicology and Teratology of Abused Toluene

Reproductive Toxicology and Teratology of Abused Toluene

Sexual behaviour is impaired by the abused inhalant toluene in adolescent male rats

The Effect of Exposure Process of Toluene To The Spermatogonia Cell a Rattus Strain Wistar

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