Toll signaling promotes JNK-dependent apoptosis in Drosophila

Li, Zhuojie; Wu, Chenxi; Xiang, Dongxi; Li, Wenzhe; Xue, Lei

doi:10.1186/s13008-020-00062-5

Cited by 21 publications

(25 citation statements)

References 70 publications

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“…We have also reported earlier that Dx interacts with the JNK pathway ligand Eiger and regulates Eiger mediated signaling activity (Dutta, Singh, et al, 2018). JNK activity is needed for Toll-induced cell death in Drosophila (Li et al 2020). Gain of JNK signaling not only inflicts cell death but also activates Toll pathway marked by an increased nuclear localization of Dorsal (Wu et al 2015).…”

Section: Co-localizes With Dorsal In the Cell Nucleus And The Nusupporting

confidence: 70%

“…JNK activity is needed for Toll‐induced cell death in Drosophila (Li et al. 2020). Gain of JNK signaling not only inflicts cell death but also activates Toll pathway marked by an increased nuclear localization of Dorsal (Wu et al.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, there are series of reports depicting the inverse relation, where Toll signaling has been seen to regulate JNK‐mediated apoptosis in flies (Li et al. 2020). However, the precise role of Dx in Toll pathway activation still needs to be determined.…”

Section: Resultsmentioning

confidence: 99%

“…In Drosophila, there is evidence of Toll regulation through JNK, where JNK signaling activates Toll pathway (Wu et al 2015); however, there is no such instance reported where JNK is shown to inhibit Toll in Drosophila. Moreover, there are series of reports depicting the inverse relation, where Toll signaling has been seen to regulate JNK-mediated apoptosis in flies (Li et al 2020). However, the precise role of Dx in Toll pathway activation still needs to be determined.…”

Section: Co-localizes With Dorsal In the Cell Nucleus And The Numentioning

confidence: 99%

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Deltex positively regulates Toll signaling in a JNK independent manner in Drosophila

2021

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Toll pathway is the center for the function of immune system in both Drosophila and mammals. Toll pathway in Drosophila gets activated upon binding of the ligand Spätzle to the receptor, Toll, triggering a series of proteolytic cascade culminating into the activation of the NF-κB factors Dorsal and/or Dif (Dorsal-related immunity factor). Inappropriate activation of the Toll pathway is often associated with systemic inflammation phenotype in the absence of infection, and thus, it is important to understand the regulation of Toll signaling. Deltex (Dx) is a context-dependent regulator of Notch signaling and has been linked with cell-mediated immunity in the mammalian system lately. However, the unambiguous role of Dx in humoral and cell-mediated immunity is yet to be explored. Our study unravels the novel role of Dx in Toll pathway activation. Gain of function of dx in Drosophila larvae results in increased melanotic mass formation and increased lamellocyte production. Our results also reveal the nuclear accumulation of transcription factors Dorsal and Dif and expression of Toll-associated antimicrobial peptides (AMP) in Dx over-expression background. Further, we also tried to elucidate the role of Dx in JNK-independent Toll activation. Here we present Dx as a novel candidate in the regulation of Toll pathway.

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Section: Co-localizes With Dorsal In the Cell Nucleus And The Nusupporting

confidence: 70%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Co-localizes With Dorsal In the Cell Nucleus And The Numentioning

confidence: 99%

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Deltex positively regulates Toll signaling in a JNK independent manner in Drosophila

2021

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“…Mechanically, emodin could protect against intestinal and lung injury induced by acute intestinal injury in rats via SP-A and TLR4/NF-κB pathway. TLRs play an important role in the regulation of phagocytosis of acute inflammatory response cells [21], cell signaling [22] and apoptosis [23]. Among all TLRs, the ligand of TLR4 is lipopolysaccharide, a cell wall component of Gram-negative bacteria.…”

Section: Discussionmentioning

confidence: 99%

Emodin protects against intestinal and lung injury induced by acute intestinal injury by modulating SP-A and TLR4/NF-κB pathway

Qian

Jin

et al. 2020

Bioscience Reports

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Objective: Our aim was to investigate the effect of emodin on intestinal and lung injury induced by acute intestinal injury in rats and explore potential molecular mechanisms. Methods: Healthy male Sprague-Dawley (SD) rats were randomly divided into five groups (n=10, each group): normal group; saline group; acute intestinal injury model group; model + emodin group; model + NF-kB inhibitor pynolidine dithiocarbamate (PDTC) group. Histopathological changes of intestine/lung tissues were observed by H&E and TUNEL staining. Serum IKBα, p-IKBα, SP-A and TLR4 levels were examined using ELISA. RT-qPCR was performed to detect the mRNA expression levels of IKBα, SP-A and TLR4 in intestine/lung tissues. Furthermore, the protein expression levels of IKBα, p-IKBα, SP-A and TLR4 were detected by western blot. Results: The pathological injury of intestinal/lung tissues was remarkedly ameliorated in models treated with emodin and PDTC. Furthermore, the intestinal/lung injury scores were significantly decreased after emodin or PDTC treatment. TUNEL results showed that both emodin and PDTC treatment distinctly attenuated the apoptosis of intestine/lung tissues induced by acute intestinal injury. At the mRNA level, emodin significantly increased the expression levels of SP-A and decreased the expression levels of IKBα and TLR4 in intestine/lung tissues. According to ELISA and western blot, emodin remarkedly inhibited the expression of p-IKBα protein and elevated the expression of SP-A and TLR4 in serum and intestine/lung tissues induced by acute intestinal injury. Conclusion: Our findings suggested that emodin could protect against intestinal and lung injury induced by acute intestinal injury by modulating SP-A and TLR4/NF-κB pathway.

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Spoonbill positively regulates JNK signalling mediated apoptosis in Drosophila melanogaster

Das

Pandey

Maurya

et al. 2023

European Journal of Cell Biology

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Toll signaling promotes JNK-dependent apoptosis in Drosophila

Cited by 21 publications

References 70 publications

Deltex positively regulates Toll signaling in a JNK independent manner in Drosophila

Deltex positively regulates Toll signaling in a JNK independent manner in Drosophila

Emodin protects against intestinal and lung injury induced by acute intestinal injury by modulating SP-A and TLR4/NF-κB pathway

Spoonbill positively regulates JNK signalling mediated apoptosis in Drosophila melanogaster

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