Toll receptors, CD14, and macrophage activation and deactivation by LPS

Dobrovolskaia, Marina A.; Vogel, Stefanie N.

doi:10.1016/s1286-4579(02)01613-1

Cited by 500 publications

(429 citation statements)

References 93 publications

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“…It is well established that CD14 is expressed on macrophages and plays an important role in the generation of immune responses to gram-negative bacteria (Dobrovolskaia and Vogel, 2002;Triantafilou and Triantafilou, 2002;Wright et al, 1990). However, recent studies have revealed that CD14 can also bind gram-positive bacterial components such as PGN and lipoteichoic acid (Cleveland et al, 1996;Dziarski, 2003;Dziarski et al, 2000a;Gupta et al, 1996).…”

Section: Effects Of S Aureus and Pgn On Cd14 Expression In Primary Mmentioning

confidence: 99%

“…The functional role of mCD14 in LPS-related macrophage responses has been studied extensively and the signal transduction mechanisms in cooperation with TLR4 are well-defined (Dobrovolskaia and Vogel, 2002;Hailman et al, 1994;Palsson-McDermott and O'Neill, 2004;Takeda et al, 2003). However, the direct functional importance of CD14 in mediating microglial responses to gram-positive stimuli has not yet been examined.…”

Section: Cd14 Differentially Regulates Pgn and S Aureus Recognition mentioning

confidence: 99%

“…Upon LPS binding, CD14 physically interacts with TLR4 to initiate intracellular signaling since the former is GPI-anchored and lacks a cytoplasmic signaling domain (Dobrovolskaia and Vogel, 2002;Palsson-McDermott and O'Neill, 2004;Triantafilou and Triantafilou, 2002). In the case of gram-positive bacterial components, evidence suggests that CD14 can enhance TLR2-dependent signaling in macrophages (Harokopakis and Hajishengallis, 2005;Manukyan et al, 2005;Schroder et al, 2003).…”

Section: Modulation Of Cd14 Ko Microglial Activation In Response To Bmentioning

confidence: 99%

“…Membrane-bound CD14 is expressed on cells of the myeloid lineage including microglia and macrophages (Becher et al, 1996;Kielian et al, 2002Kielian et al, , 2005Nadeau and Rivest, 2000;Saito et al, 2000;Tsan et al, 2001). It has been well established that mCD14 plays a pivotal role in enabling cells to recognize the gram-negative bacterial cell wall component lipopolysaccharide (LPS) (Dobrovolskaia and Vogel, 2002;Palsson-McDermott and O'Neill, 2004). Since mCD14 is a glycosylphosphatidyl inositol (GPI)-anchored receptor, it requires interaction with TLR4 to transduce activation signals in response to LPS (Dobrovolskaia and Vogel, 2002;Fitzgerald et al, 2004;Haziot et al, 1988;Palsson-McDermott and O'Neill, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…It has been well established that mCD14 plays a pivotal role in enabling cells to recognize the gram-negative bacterial cell wall component lipopolysaccharide (LPS) (Dobrovolskaia and Vogel, 2002;Palsson-McDermott and O'Neill, 2004). Since mCD14 is a glycosylphosphatidyl inositol (GPI)-anchored receptor, it requires interaction with TLR4 to transduce activation signals in response to LPS (Dobrovolskaia and Vogel, 2002;Fitzgerald et al, 2004;Haziot et al, 1988;Palsson-McDermott and O'Neill, 2004). However, recent evidence also supports a role for mCD14 in the recognition of gram-positive PAMPs such as PGN and lipoteichoic acid (Cleveland et al, 1996;Dziarski et al, 2000a;Gupta et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Recognition of Staphylococcus aureus-derived peptidoglycan (PGN) but not intact bacteria is mediated by CD14 in microglia

Esen

Kielian

2005

Journal of Neuroimmunology

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Recognition of Staphylococcus aureus and its cell-wall component peptidoglycan (PGN) by microglia is mediated, in part, by Toll-like receptor 2 (TLR2). However, the pattern recognition receptor (PRR) CD14 can also bind PGN and enhance TLR2-mediated signaling in macrophages, suggesting a similar phenomenon might occur in microglia. To assess the functional significance of CD14 on microglial activation, we evaluated the responses of primary microglia isolated from CD14 knockout (KO) and wild type (WT) mice. PGN-dependent microglial activation was partially CD14-dependent as demonstrated by the attenuated expression of TNF-α, macrophage inflammatory protein-2 (MIP-2/CXCL2), and the soluble PRR pentraxin-3 in CD14 KO microglia compared to WT cells. In contrast, microglial responses to intact S. aureus occurred primarily via a CD14-independent manner. Collectively, these findings reveal the complex nature of gram-positive bacterial recognition by microglia, which occurs, in part, via CD14.

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Section: Effects Of S Aureus and Pgn On Cd14 Expression In Primary Mmentioning

confidence: 99%

Section: Cd14 Differentially Regulates Pgn and S Aureus Recognition mentioning

confidence: 99%

Section: Modulation Of Cd14 Ko Microglial Activation In Response To Bmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Recognition of Staphylococcus aureus-derived peptidoglycan (PGN) but not intact bacteria is mediated by CD14 in microglia

Esen

Kielian

2005

Journal of Neuroimmunology

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The E3 ubiquitin ligase MuRF2 attenuates LPS‐induced macrophage activation by inhibiting production of inflammatory cytokines and migration

et al. 2018

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Muscle RING‐finger (MuRF) proteins are E3 ubiquitin ligases that are expressed in striated muscle. MuRF2 is an important member of this family, but whether it is expressed in tissues other than striated muscle has not been thoroughly elucidated to date. In this study, we determined that Mu RF 2 is also expressed in other vital organs, including liver, lung, brain, spleen and kidney. Moreover, we show that the level of Mu RF 2 expression is significantly decreased in hepatic mononuclear cells of mice with lipopolysaccharide ( LPS )/ d ‐galactosamine‐induced hepatitis and negatively correlated with the serum levels of alanine aminotransferase and aspartate aminotransferase in these mice. Furthermore, the expression of Mu RF 2 was down‐regulated in RAW 264.7 cells activated with LPS but not in cells treated with polyinosinic‐polycytidylic acid (Poly(I:C)) or with lipidosome plus Poly(I:C). We also found that Mu RF 2 was able to translocate from the cytoplasm to the nucleus in RAW 264.7 cells activated with LPS but not in cells treated with Poly(I:C). In addition, we demonstrated that interleukin 6 and tumour necrosis factor α production and macrophage migration were inhibited after MuRF2 was overexpressed in RAW 264.7 cells. We further verified that nuclear factor‐κB p65 subunit level was greatly reduced in RAW 264.7 macrophage nuclei by gain of function. Taken together, these findings indicate that Mu RF 2 may rescue LPS ‐induced macrophage activation by suppressing the production of proinflammatory cytokines and cell migration. We also identify a novel function of Mu RF 2 in non‐muscle tissues and cells.

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Click Chemistry Applied to Carbohydrate‐Based Drug Discovery

Campo¹,

Carvalho²

2013

Click Chemistry in Glycoscience

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Toll receptors, CD14, and macrophage activation and deactivation by LPS

Cited by 500 publications

References 93 publications

Recognition of Staphylococcus aureus-derived peptidoglycan (PGN) but not intact bacteria is mediated by CD14 in microglia

Recognition of Staphylococcus aureus-derived peptidoglycan (PGN) but not intact bacteria is mediated by CD14 in microglia

The E3 ubiquitin ligase MuRF2 attenuates LPS‐induced macrophage activation by inhibiting production of inflammatory cytokines and migration

Click Chemistry Applied to Carbohydrate‐Based Drug Discovery

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