Toll-like receptor 4-dependent activation of macrophages by polysaccharide isolated from the radix of Platycodon grandiflorum

Yoon, Yeo Dae; Han, Sang Bae; Kang, Jong Soon; Lee, Chang Woo; Park, Song‐Kyu; Lee, Hyun Sun; Kang, Jong Seong; Kim, Hwan Mook

doi:10.1016/j.intimp.2003.09.005

Cited by 120 publications

(74 citation statements)

References 34 publications

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“…TLRs have been revealed to be expressed on various lymphocytes, and TLR2 and TLR4 are expressed on T cells; TLR2/4 is generally considered to be the main receptor of polysaccharides (20,21). To determine whether TLR2 or TLR4 was involved in the enhancement of splenocyte proliferation by SEP-S, blocking experiments were performed using anti-TLR2 and anti-TLR4 antibodies.…”

Section: Sep-s Upregulates T Lymphocyte Subsets From the Spleen And Tmentioning

confidence: 99%

A polysaccharide component from Strongylocentrotus nudus eggs inhibited hepatocellular carcinoma in mice by activating T lymphocytes

Zhang

et al. 2017

Oncology Letters

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Abstract.A component purified from Strongylocentrotus nudus eggs on a diethylaminoethyl cellulose-52 chromatography column and eluted using a NaCl solution gradient (SEP-S), is a homogeneous polysaccharide of α-D-glucan with a reduced molecular weight of 9.33x105 Da, compared with that of S. nudus egg polysaccharide (SEP). In an in vivo antitumor assay of histocompatibility-22 hepatocellular carcinoma in tumor-bearing mice, the inhibitory rates at SEP-S doses of 5, 10 and 20 mg/kg/day were 38.8, 50.7 and 70.3%, respectively. In addition, the spleen and thymus indices and the percentages of cluster of differentiation (CD) 4 + and CD8 + T cells were significantly increased, and the activity of cytotoxic T lymphocytes was notably enhanced, suggesting that the anti-hepatocellular carcinoma activity is mediated by boosting the immune system. In vitro experiments also demonstrated that splenocyte proliferation induced by SEP-S was inhibited by the toll-like receptor (TLR) 2 and TLR4 monoclonal antibodies. These data indicate that SEP-S is a polysaccharide component possessing high anti-hepatocellular carcinoma activity and may be a potential immunotherapy candidate for the treatment of liver cancer.

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Section: Sep-s Upregulates T Lymphocyte Subsets From the Spleen And Tmentioning

confidence: 99%

A polysaccharide component from Strongylocentrotus nudus eggs inhibited hepatocellular carcinoma in mice by activating T lymphocytes

Zhang

et al. 2017

Oncology Letters

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“…19) In the present study, we evaluated the effects of PG, which is widely distributed in northeast Asia and has long been used as a traditional Oriental medicine for the treatment of pulmonary and respiratory disorders, such as bronchitis, tonsillitis, and asthma. 20,21) Pharmacological studies of the root of PG have revealed that it possesses immunomodulatory [22][23][24] and antiinflammatory 25,26) properties. The lactic acid bacteria (LAB), LP, which are non-invasive and non-pathogenic Gram-positive commensal microorganisms, are recognized as health-promoting microorganisms.…”

Section: Abstract Atopic Dermatitis; Lactobacillus Plantarum; Nc/ngamentioning

confidence: 99%

Improvement of Atopic Dermatitis-Like Skin Lesions by Platycodon grandiflorum Fermented by Lactobacillus plantarum in NC/Nga Mice

Kim¹,

Kim

Chung

et al. 2012

Biological & Pharmaceutical Bulletin

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“…Recently, plant-derived polysaccharides have been paid more and more attention due to their anti-cancer activities and their capability to improve the body's immunomodulation (Yoon et al, 2003;Nergard et al, 2004;Nam et al, 2009;Du et al, 2012). In oriental medicine, there has been a long history of herbal use to prevent and treat diseases, including cancers, by modulating the body's natural immune-defence system.…”

Section: Introductionmentioning

confidence: 99%

“…It was revealed that, among those herbs, polysaccharides are the major active ingredients exerting therapeutic efficacy. For example, polysaccharides extracted from Sparassis crispa (Harada et al, 2002), Platycodon grandiflorum (Yoon et al, 2003), Vernonia kotschyana (Nergard et al, 2004), Asterina pectinifera (Kim,et al, 2012) and more have been demonstrated to possess anti-cancer activities.…”

Section: Introductionmentioning

confidence: 99%

Anti-tumour effects of polysaccharides isolated from Artemisia Annua L by inducing cell apoptosis and immunomodulatory anti-hepatoma effects of polysaccharides

Chen¹,

Chen²,

Wang³

et al. 2013

Afr. J. Trad. Compl. Alt. Med.

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Background: It is well known that various polysaccharides present anti-tumour effects by inducing cell apoptosis and immunomodulation. However, it is still unclear about the roles of polysaccharides isolated from Artemisia apiacea (HQG) to hepatoma and its underlying mechanism. The objective of the study was to examine the anti-hepatoma effects of HQG and its related mechanism. Materials and Methods: HQG was prepared in house and the quality and purity were confirmed by infra-red spectrum and gel permeation chromatography (GPC). Tumour-bearing mice induced by injection of mouse hepatoma H22 cells were used to evaluate the tumour growth inhibition by HQG administration. Cell immunostaining, JC1 staining and flow cytometer were performed to examine the cell apoptosis, mitochondrial membrane potential change and immunomodulation in response to HQG treatment. Results: HQG treatment inhibited hepatoma growth in tumour-bearing mice. Cell apoptosis rate of human hepatoma 7402 cells and of the cells from ascites in tumour-bearing mice was increased after HQG treatment. Mitochondrial membrane potential in human hepatoma 7402 cells was decreased after HQG treatment. CD4+ and CD8+ T-lymphocytes subpopulation was increased while the ratio of CD4+/ CD8+ decreased in tumour-bearing mice after HQG administration. IFN-γ and IL-4 secretion was increased in spleen lymphocytes in tumour-bearing mice after HQG administration. Conclusion:The study concluded that polysaccharides isolated from Artemisia apiacea (HQG) can inhibit hepatoma cell growths by facilitating cell apoptosis and immuno-defence.

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Toll-like receptor 4-dependent activation of macrophages by polysaccharide isolated from the radix of Platycodon grandiflorum

Cited by 120 publications

References 34 publications

A polysaccharide component from Strongylocentrotus nudus eggs inhibited hepatocellular carcinoma in mice by activating T lymphocytes

A polysaccharide component from Strongylocentrotus nudus eggs inhibited hepatocellular carcinoma in mice by activating T lymphocytes

Improvement of Atopic Dermatitis-Like Skin Lesions by <i>Platycodon grandiflorum</i> Fermented by <i>Lactobacillus plantarum</i> in NC/Nga Mice

Anti-tumour effects of polysaccharides isolated from <i>Artemisia Annua</i> L by inducing cell apoptosis and immunomodulatory anti-hepatoma effects of polysaccharides

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