Toll-like receptor 3 polymorphism is not associated with neovascular age-related macular degeneration and polypoidal choroidal vasculopathy in the Chinese

Abstract

ABSTRACT. Toll-like receptor 3 (TLR3) variants in mainland northern Chinese patients with polypoidal choroidal vasculopathy (PCV) and neovascular age-related macular degeneration (nAMD) were investigated. The complete genes of TLR3, including all exons and the promoter region, were assessed using direct sequencing technology of 284 unrelated mainland northern Chinese individuals: 96 nAMD patients, 92 PCV patients, and 96 controls. Six single TLR3 is not associated with nAMD and PCV nucleotide polymorphisms were identified: rs5743303, rs5743305, rs5743312, rs3775291, rs3775290, and rs6830345. The distribution of TLR3 genotypes for nAMD and PCV was not significantly different compared with normal controls. This study indicates that the TLR3 gene polymorphism is not associated with nAMD and PCV in northern Chinese patients.

Association of toll-like receptor 3 polymorphism rs3775291 with age-related macular degeneration: a systematic review and meta-analysis

“…Therefore, only the latest article was included for meta-analysis 19 . Finally, a total of 9 studies with 25 case-control cohorts were included, involving 7400 cases and 13579 controls 8 11 12 13 14 15 16 19 20 .…”

“…Three SNPs, rs3775291, rs5743303 and rs5743312, have been reported in at least two studies and are thus eligible for meta-analysis. The other 9 SNPs (rs4986790, rs5743305, rs3775290, rs11721827, rs11730143, rs11732384, rs13126816, rs10025405 and rs6830345) were investigated in single studies, in which they were reported to have no significant association with AMD 8 13 14 .…”

“…TLR3 rs3775291 was reported in 6 studies with totally 11 cohorts 8 12 13 14 15 16 , including 2746 nAMD cases and 3507 controls ( Table 3 ). Meta-analysis showed that this SNP was associated with nAMD in the recessive model (P = 0.01, OR = 0.78, 95% CI: 0.64–0.94; Fig.…”

“…In a meta-analysis involving 3 reports, the T allele of rs3775291 had a protective effect for GA (odds ratio [OR] = 0.75, 95% confidence interval [CI] = 0.62–0.93) 7 . Recently, a lot more new data on rs3775291 have been reported in GA and other AMD subtypes 13 14 15 16 , revealing inconsistent association profiles in different study cohorts. Therefore, we conducted a meta-analysis to evaluate the role of TLR3 SNPs, including rs3775291 and others, on different subtypes of AMD.…”

Retinal Pigment Epithelium Expressed Toll-like Receptors and Their Potential Role in Age-Related Macular Degeneration

“…While this association may be of importance for Caucasian subpopulations, no association has been found in a study with Indian subjects [52], supported by another meta-analysis, which showed an association with Caucasian, but not Asian subjects [53]. Moreover, no association between the polymorphism of TLR3 has been drawn to the neovascular subtype of AMD, as shown for Chinese study populations [54,55] or with the growth of GA in AMD patients treated with anti-VEGF compounds (American study population of 43 different centers) [56]. In an exhaustive study investigating 68 SNPs of several TLRs, associations of some SNPs, e.g., for TLR3 and TLR7 were shown to be statistically significant in one cohort of patients (Dallas cohort), but not in another cohort (Michigan cohort) [48].…”