Tolerance to Pierce’s Disease and the Associated Rickettsia-like Bacterium in Muscadine Grape1

Hopkins, D. L.; Mollenhauer, Hilton H.; Mortensen, J. A.

doi:10.21273/jashs.99.5.436

Cited by 21 publications

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“…In comparison, the subgenus Euvitis (bunch grapes) exhibits greater diversity and includes about 60 species. Compared to the common table grape ( V. vinifera ; a member of the Euvitis subgenus), muscadine grapes are a small-market crop [ 1 ], but they are also more pest and drought tolerant [ 2 ]. Based on phylogenetic evidence, the Vitis genus originated in North America 28 million years ago, and the Muscadinia subgenus diverged from Euvitis approximately 18 million years ago [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Long-read, chromosome-scale assembly of Vitis rotundifolia cv. Carlos and its unique resistance to Xylella fastidiosa subsp. fastidiosa

et al. 2023

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Background Muscadine grape (Vitis rotundifolia) is resistant to many of the pathogens that negatively impact the production of common grape (V. vinifera), including the bacterial pathogen Xylella fastidiosa subsp. fastidiosa (Xfsf), which causes Pierce’s Disease (PD). Previous studies in common grape have indicated Xfsf delays host immune response with a complex O-chain antigen produced by the wzy gene. Muscadine cultivars range from tolerant to completely resistant to Xfsf, but the mechanism is unknown. Results We assembled and annotated a new, long-read genome assembly for ‘Carlos’, a cultivar of muscadine that exhibits tolerance, to build upon the existing genetic resources available for muscadine. We used these resources to construct an initial pan-genome for three cultivars of muscadine and one cultivar of common grape. This pan-genome contains a total of 34,970 synteny-constrained entries containing genes of similar structure. Comparison of resistance gene content between the ‘Carlos’ and common grape genomes indicates an expansion of resistance (R) genes in ‘Carlos.’ We further identified genes involved in Xfsf response by transcriptome sequencing ‘Carlos’ plants inoculated with Xfsf. We observed 234 differentially expressed genes with functions related to lipid catabolism, oxidation-reduction signaling, and abscisic acid (ABA) signaling as well as seven R genes. Leveraging public data from previous experiments of common grape inoculated with Xfsf, we determined that most differentially expressed genes in the muscadine response were not found in common grape, and three of the R genes identified as differentially expressed in muscadine do not have an ortholog in the common grape genome. Conclusions Our results support the utility of a pan-genome approach to identify candidate genes for traits of interest, particularly disease resistance to Xfsf, within and between muscadine and common grape.

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Section: Introductionmentioning

confidence: 99%

Long-read, chromosome-scale assembly of Vitis rotundifolia cv. Carlos and its unique resistance to Xylella fastidiosa subsp. fastidiosa

et al. 2023

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“…The popularity of musca dine grapes in this area depends primarily on their high level of tolerance to PD, which is exemplified by their excellent vigor and lon gevity (5). The presence, however, of the xylem-limited bacterium that causes PD has been reported in several muscadine cultivars (4,7).…”

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Seasonal Concentration of the Pierce’s Disease Bacterium in ‘Carlos’ and ‘Welder’ Muscadine Grapes Compared with ‘Schuyler’ Bunch Grape

Hopkins

Thompson

1984

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The accumulation of Pierce’s disease bacterial occlusions in xylem vessels of leaves of susceptible ‘Schuyler’ bunch grapes occurred 2 months earlier than the accumulation in leaf vessels of the more tolerant ‘Carlos’ and ‘Welder’ muscadines. The first appearance of leaf marginal necrosis symptoms occurred when the accumulation of bacterial occlusions was approaching the maximum—the last week of June in the bunch grape, the last week of Aug. in the 2 muscadines. The site of bacterial accumulation was primarily in the leaves of ‘Carlos’, but in both leaves and stems of ‘Schuyler’ and ‘Welder’. Stem dieback symptoms are more common in the latter 2 cultivars.

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Breeding Muscadine Grapes

Goldy

1992

Horticultural Reviews

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Tolerance to Pierce’s Disease and the Associated Rickettsia-like Bacterium in Muscadine Grape1

Cited by 21 publications

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Long-read, chromosome-scale assembly of Vitis rotundifolia cv. Carlos and its unique resistance to Xylella fastidiosa subsp. fastidiosa

Long-read, chromosome-scale assembly of Vitis rotundifolia cv. Carlos and its unique resistance to Xylella fastidiosa subsp. fastidiosa

Seasonal Concentration of the Pierce’s Disease Bacterium in ‘Carlos’ and ‘Welder’ Muscadine Grapes Compared with ‘Schuyler’ Bunch Grape

Breeding Muscadine Grapes

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