Tolerance to acetic acid is improved by mutations of the TATA‐binding protein gene

Abstract

Screening a library of overexpressing mutant alleles of the TATA-binding gene SPT15 yielded two Saccharomyces cerevisiae strains (MRRC 3252 and 3253) with enhanced tolerance to acetic acid. They were also tolerant to propionic acid and hydrogen peroxide. Transcriptome profile analysis identified 58 upregulated genes and 106 downregulated genes in MRRC 3252. Stress- and protein synthesis-related transcription factors were predominantly enriched in the upregulated and downregulated genes respectively. Eight deletion mutants for some of the highly downregulated genes were acetic acid-tolerant. The level of intracellular reactive oxygen species was considerably lessened in MRRC 3252 and 3253 upon exposure to acetic acid. Metabolome profile analysis revealed that intracellular concentrations of 5 and 102 metabolites were increased and decreased, respectively, in MRRC 3252, featuring a large increase of urea and a significant decrease of amino acids. The dur1/2Δmutant, in which the urea degradation gene DUR1/2 is deleted, displayed enhanced tolerance to acetic acid. Enhanced tolerance to acetic acid was also observed on the medium containing a low concentration of amino acids. Taken together, this study identified two SPT15 alleles, nine gene deletions and low concentration of amino acids in the medium that confer enhanced tolerance to acetic acid.

Adaptive Response and Tolerance to Acetic Acid in Saccharomyces cerevisiae and Zygosaccharomyces bailii: A Physiological Genomics Perspective

“…The remodeling of the yeast transcriptome through global transcription machinery engineering has also been applied successfully to obtain acetic acid-tolerant strains. This was achieved by re-programming the cell transcriptome through mutations in SPT15 , encoding the TATA-binding protein, followed by screening of the mutants with improved tolerance to acetic acid ( An et al, 2015 ). Another example involved the transformation of a yeast strain with an artificial zinc finger protein transcription factor library and subsequent selection of acetic acid-tolerant strains ( Ma et al, 2015 ).…”

Introns provide a platform for intergenic regulatory feedback of RPL22 paralogs in yeast

“…Rpl22 has been recently shown to directly bind and block MDM2 [ 53 ], which is intriguing, given the occurrence of inactivating mutations of RPL22 in various cancers [ 53 , 54 ]. In yeast, RPL22A deletion increases replicative lifespan [ 18 ] and affects sensitivity to oxidative stress [ 55 , 56 ] and acetic acid [ 57 ]. Furthermore, Rpl22 has been implicated in translation regulation of meiotic inducer IME1 .…”

Improved growth and ethanol fermentation of Saccharomyces cerevisiae in the presence of acetic acid by overexpression of SET5 and PPR1

“…It has been known that multiple genes are involved in acetic acid tolerance of S. cerevisiae [7–9], but few studies have been conducted on whether the overexpression of key genes can improve acetic acid tolerance [9–13]. Recently, global transcriptional machinery engineering (gTME) was developed to improve stress tolerance, and ethanol tolerance of S. cerevisiae was significantly improved by manipulation of one single gene of SPT15 [14].…”