This study aims to determine the effects of planting media and gutter slopes on the growth of lettuce (Lactuca sativa L.) using the Nutrient Film Technique (NFT) in a hydroponic system. This research was conducted at the Experimental Garden of the Faculty of Agriculture, Universitas Lakidende Unaaha, Konawe Regency, Indonesia, in October 2021. The two treatments were arranged in a randomized block design (RBD) and repeated in three replications. The first treatment consisted of two types of growing media: sponge medium (M1) and rockwool medium (M2). The second treatment consisted of two kinds, i.e., a 3% gutter slope of pipes (K1) and a 5% gutter slope of pipes (K2). The observed variables were plant height, number of leaves, and plant fresh weight. All collected data were analyzed using analysis of variance (ANOVA) followed by the least significant difference (LSD) test at a 5% level. This study has revealed three major results. Firstly, the interaction between planting media and the slope of the gutter pipes significantly affects plant height and the fresh weight of lettuce. Secondly, the planting media or the slope of the gutter pipes independently has a significant effect on plant height, several leaves, and the fresh weight of lettuce plants. Lastly, the treatment of rockwool planting media and a 5% slope of gutter pipes significantly improved the lettuce growth.