Strawberries (Fragaria ×ananassa) are one of the major high-value crops in North America. There is increasing interest in commercial strawberry production for local markets in Virginia and surrounding states, but information on the performance of newer cultivars is extremely limited. We tested 10 commercially available June-bearing cultivars [Benicia, Camarosa, Camino Real, Chandler, Strawberry Festival, Flavorfest, FL Radiance, Treasure, Sweet Charlie, and Winterstar™ (FL 05-107)] and two day-neutral cultivars (Albion and San Andreas) for their spring and summer fruiting capacity in Virginia production systems in a randomized, replicated study, at three on-farm locations. Data were collected on vegetative growth, yield performance, fruit quality, sweetness, and fruit diameter. Cultivars with the highest total yields averaged across all three locations were Benicia, Camino Real, Chandler, and Camarosa. ‘Camino Real’ had the highest marketable yield at all three locations, not significantly different from ‘Chandler’, and ‘Benicia’ and ‘Camarosa’ had the highest marketable yield at two of the three locations. ‘Flavorfest’ and ‘Sweet Charlie’ had the highest total soluble solids concentration for the harvest season. Overall, for all locations, ‘Benicia’ and ‘Camino Real’ had the largest fruit diameter, and ‘Strawberry Festival’ had the smallest fruit diameter.