Tolerance of Microbial Biocatalysts to Feedstocks, Products, and Environmental Conditions

Huang, Mian; Peabody, George L.; Kao, Katy C.

doi:10.1007/978-3-319-41966-4_5

Cited by 4 publications

(1 citation statement)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Generally, the bioprocess perturbation space has a negative impact on strain performance compared with its ideal and well-controlled lab-scale space, both in terms of specific growth rates and production of valuable metabolites. Therefore, industrial strains must exhibit consistent performance, that is, robustness, to avoid loss of product quantity and quality or larger costs ( Huang et al, 2016 ; Mohedano et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Performance and robustness analysis reveals phenotypic trade-offs in yeast

Trivellin,

Rugbjerg,

Olsson

2023

Life Sci. Alliance

View full text Add to dashboard Cite

To design strains that can function efficiently in complex industrial settings, it is crucial to consider their robustness, that is, the stability of their performance when faced with perturbations. In the present study, we cultivated 24Saccharomyces cerevisiaestrains under conditions that simulated perturbations encountered during lignocellulosic bioethanol production, and assessed the performance and robustness of multiple phenotypes simultaneously. The observed negative correlations confirmed a trade-off between performance and robustness of ethanol yield, biomass yield, and cell dry weight. Conversely, the specific growth rate performance positively correlated with the robustness, presumably because of evolutionary selection for robust, fast-growing cells. The Ethanol Red strain exhibited both high performance and robustness, making it a good candidate for bioproduction in the tested perturbation space. Our results experimentally map the robustness–performance trade-offs, previously demonstrated mainly by single-phenotype and computational studies.

show abstract

Section: Introductionmentioning

confidence: 99%