Tolerance of dormant and active cells in Pseudomonas aeruginosa PA01 biofilm to antimicrobial agents

Kim, Jaeeun; Hahn, Jinyong; Franklin, Michael J.; Stewart, Philip S.; Yoon, Jeyong

doi:10.1093/jac/dkn462

Cited by 99 publications

(59 citation statements)

References 22 publications

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“…Our results demonstrate that both formulations are able to penetrate the deeper layers of the biofilm and thus access a dormant and anaerobically growing subpopulation. 27,28 A reduction of the free colistin concentration, the delayed penetration of the free drug into the deeper portions of the multilayered biofilm, and the lack of specificity of NLC-colistin in killing metabolically active bacteria versus starved cells may account for our results, as proposed in similar studies. 17,29 Further experiments will be aimed at improving the experimental conditions to optimize the performance of NLC-colistin.…”

Section: Resultssupporting

confidence: 68%

Determination of the spatiotemporal dependence of <em>Pseudomonas aeruginosa</em> biofilm viability after treatment with NLC-colistin

Sans-Serramitjana¹,

Jorba²,

Pedraz³

et al. 2017

IJN

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The emergence of colistin-resistant Pseudomonas aeruginosa in cystic fibrosis (CF) patients, particularly after long-term inhalation treatments, has been recently reported. Nanoen-capsulation may enable preparations to overcome the limitations of conventional pharmaceutical forms. We have determined the time-dependent viability of P. aeruginosa biofilms treated with both free and nanoencapsulated colistin. We also examined the relationship between the optimal anti-biofilm activity of nanostructured lipid carrier (NLC)-colistin and the structural organization of the biofilm itself. The results showed the more rapid killing of P. aeruginosa bacterial biofilms by NLC-colistin than by free colistin. However, the two formulations did not differ in terms of the final percentages of living and dead cells, which were higher in the inner than in the outer layers of the treated biofilms. The effective anti-biofilm activity of NLC-colistin and its faster killing effect recommend further studies of its use over free colistin in the treatment of P. aeruginosa infections in CF patients.

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Section: Resultssupporting

confidence: 68%

Determination of the spatiotemporal dependence of <em>Pseudomonas aeruginosa</em> biofilm viability after treatment with NLC-colistin

Sans-Serramitjana¹,

Jorba²,

Pedraz³

et al. 2017

IJN

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“…Protection of species one another are then assumed to increase biofilm resistance to chemical and mechanical treatment (Simoes et al, 2009;Vlkova et al, 2008). Nevertheless, according to Kim et al (2008) the major reason for antimicrobial tolerance of biofilms is the presence of dormant cells. Indeed, physiological heterogeneity in biofilms has been reported .…”

Section: Inactivation Of B Cereus Biofilms By Sanitizermentioning

confidence: 99%

Microflora of biofilm on Algerian dairy processing lines: An approach to improve microbial quality of pasteurized milk

Malek¹,

Moussa–Boudjemâa²,

Khaouani-Yousfi³

et al. 2012

Afr. J. Microbiol. Res.

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, 2.5 × 10 5 and 9.7 × 10 7 cfu/cm 2 , respectively, in the different units. Phenotypic identification of isolates revealed predominance of Gram-positive bacilli belonging to Bacillus and notably the Bacillus cereus group, at maximal levels of 72 and 21% respectively. The other Gram-positive microflora included Staphylococcus (30%) and Micrococcus (10%). In contrast, the incidence of the Gram-negative bacteria was relatively low. Two genera, identified as Pseudomonas (9%) and Enterobacter (6%), were found only in two dairies. Three dairies were Gram-negative bacteria-free. That should be the result of common contamination sources or highly environmental selective pressure. Further studies have to address these hypotheses. Treatment of experimental Bacillus cereus sensu lato strains biofilms with a 50, 100 and 150 ppm of quaternary ammonium disinfectant, showed a significant resistance of biofilms to this product even after long exposure time (15 min). This study emphasized the importance of aerobic spore-forming bacteria in dairy-processing equipment as they are able to built recalcitrant biofilms on the inside equipment surfaces with subsequent resistance to conventional CIP system and potential transfer to pasteurized milk. Therefore, in order to reduce the contamination levels of spore-forming bacteria and improve the quality and shelf life of the product, these dairies have, besides improvement in the hygienic status of the plant equipments, also to monitor either the pasteurization process or the contamination from raw material (that is, milk powder).

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“…The factors, which might induce bacterial cell to be VBNC, are less nutrients, nucleotide, inactive cell membrane, size of bacterial cells and presence of antibiotics, which inhibit DNA synthesis without affecting other cellular metabolic activities (Gasoll et al 1995;Joux and LeBaron 1997;Luna et al 2002;Kim et al 2009). Therefore, some authors recommended using molecular techniques such as PCR to detect the presence of VBNC bacteria after disinfection process (Straub et al 1993;Choi et al 1996).…”

Section: Pathogen Growth Potential (Pgp)mentioning

confidence: 99%

Removal of pathogenic bacteria from sewage-treated effluent and biosolids for agricultural purposes

et al. 2018

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The reuse of treated sewage for irrigation is considered as an important alternative water source in the new water management strategy of the countries that face a severe deficiency of water resources such as the Middle East countries. The organic material and fertilizing elements contained in biosolids are essential for maintaining soil fertility. However, both treated sewage and biosolids contain a large diversity of pathogens that would be transmitted to the environment and infect human directly or indirectly. Therefore, those pathogens should be reduced from the treated sewage and biosolids before the reuse in the agriculture. This paper reviews the considerations for reuse of treated sewage and biosolids in agriculture and further treatments used for reduction of pathogenic bacteria. The treatment methods used for the reduction of pathogens in these wastes have reviewed. It appeared that the main concern associated with the reduction of pathogenic bacteria lies in their ability to regrow in the treated sewage and biosolids. Therefore, the effective treatment method is that it has the potential to destruct pathogens cells and remove the nutrients to prevent the regrowth or recontamination from the surrounded environment. The removal of nutrients might be applicable in the sewage but not in the biosolids due to high nutrient contents. However, the reduction of health risk in the biosolids might be carried out by regulating the biosolid utilization and selecting the plant species grown in the fertilized soil with biosolids.

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Tolerance of dormant and active cells in Pseudomonas aeruginosa PA01 biofilm to antimicrobial agents

Cited by 99 publications

References 22 publications

Determination of the spatiotemporal dependence of <em>Pseudomonas aeruginosa</em> biofilm viability after treatment with NLC-colistin

Determination of the spatiotemporal dependence of <em>Pseudomonas aeruginosa</em> biofilm viability after treatment with NLC-colistin

Microflora of biofilm on Algerian dairy processing lines: An approach to improve microbial quality of pasteurized milk

Removal of pathogenic bacteria from sewage-treated effluent and biosolids for agricultural purposes

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