Tolerance of an energy crop (Jatropha curcas L.) to zinc and lead assessed by chlorophyll fluorescence and enzyme activity

Marques, Marise Conceição; Nascimento, Clístenes Williams Araújo do; Silva, A.J. da; Gouveia-Neto, Artur da Silva

doi:10.1016/j.sajb.2017.06.009

Cited by 18 publications

(11 citation statements)

References 52 publications

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“…In relation to the photosynthetic machinery, decreases in the photochemical efficiency and the activities of ribulose-1,5-bisphosphate carboxylase/ oxygenase (RuBisCO) and carbonic anhydrase (CA) enzymes have been reported (Salama et al 2006;Tavallali et al 2009;Hajiboland and Amirazad 2010). However, excess levels of Zn also promote deleterious effects on crop yield (Tripathi et al 2015) because Zn toxicity negatively affects CO 2 assimilation and stomatal mechanisms (Azzarello et al 2012), thus decreasing the transpiration rates and water content in the leaf (Sagardoy et al 2009) and resulting in a lower biomass (Marques et al 2017).…”

Section: Introductionmentioning

confidence: 99%

24-Epibrassinolide Improves Root Anatomy and Antioxidant Enzymes in Soybean Plants Subjected to Zinc Stress

Santos

Silva

Pedron

et al. 2019

J Soil Sci Plant Nutr

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The aim of this research was to determine whether 24-epibrassinolide can mitigate oxidative stress in soybean plants subjected to different zinc levels; to examine this, we evaluated the possible repercussions on anatomical, nutritional, biochemical, physiological and morphological behaviours. The experiment followed a completely randomized factorial design with two concentrations of 24-epibrassinolide (0 and 100 nM EBR, described as-EBR and + EBR, respectively) and three zinc supplies (0.2, 20 and 2000 μM Zn, described as low, control and a high supply of Zn). In general, low and high zinc supplies produced deleterious effects. However, plants exposed to high zinc +100 nM EBR presented increases of 25%, 7%, 9% 29% and 69% for root epidermis, root endodermis, root cortex, vascular cylinder and metaxylem, respectively, when compared to the same treatment without the steroid. The steroid spray alleviated the impact produced by zinc stress on nutritional status, and these results were intrinsically linked to incremental changes in root structure, mainly vascular cylinder and metaxylem. Antioxidant enzymes play crucial roles in the photosynthetic machinery of plants treated with 24-epibrassinolide and stressed by high and low zinc supply, modulating reactive oxygen species scavenging and protecting the chloroplast membranes, with clear positive repercussions on photosystem II efficiency and photosynthetic pigments. The stimulation induced by this steroid on gas exchange can be explained by the favourable conditions detected in stomatal performance and leaf anatomy, thus enhancing the diffusion of carbon dioxide.

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Section: Introductionmentioning

confidence: 99%

24-Epibrassinolide Improves Root Anatomy and Antioxidant Enzymes in Soybean Plants Subjected to Zinc Stress

Santos

Silva

Pedron

et al. 2019

J Soil Sci Plant Nutr

View full text Add to dashboard Cite

show abstract

“…Chlorophyll fluorescence is a highly informative technique of plant traits to cope with adverse environmental conditions [10]. In this sense, chlorophyll fluorescence uses information about the photochemical activity of plants, allowing the early detection of environmental stress [11]. This can be done because the chlorophyll molecule is fluorescent, which makes it possible to detect changes in electron transfer at the level of chloroplast membranes through photon dissipation [12].…”

Section: Introductionmentioning

confidence: 99%

Chlorophyll α Fluorescence Parameters as an Indicator to Identify Drought Susceptibility in Common Bush Bean

Sánchez-Reinoso¹,

Moreno²,

Restrepo-Díaz³

2019

Agronomy

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The common bean is susceptible to drought conditions and the evaluation of plant responses to low water availability can be difficult. The quantification of chlorophyll fluorescence as a sensitive trait to environmental stresses is an important alternative in the characterization of drought-susceptible genotypes. The objective of this study was to evaluate mainly the use of chlorophyll α fluorescence (maximum efficiency of PSII (Fv/Fm), photochemical quenching (qP), non-photochemical quenching (NPQ)) and rapid light-response curves (RLCs) (initial slope of the curve (α), minimum saturation irradiance (Ik) and maximum relative electron transport rate (ETRmax)) parameters as tools for the identification of susceptible or tolerant bush bean cultivars to water deficit stress conditions in two different phenological stages. Using a randomized block design in a factorial arrangement, five bush bean cultivars (Cerinza, Bachue, NUA35, Bacata and Bianca) were evaluated under water deficit conditions by the suspension of irrigation for 15 days from 40 to 55 Days after Emergence (DAE) (vegetative stage) or 50 to 65 DAE (reproductive stage). The results showed that Fv/Fm and NPQ recorded the highest variation due to water deficit conditions, especially in the vegetative stage. The greatest reductions in Fv/Fm (0.67) and NPQ (0.71) were evidenced in cultivar NUA35 compared to its control plants (0.78 and 1.07, respectively). The parameters obtained from RLCs showed that cultivar Bacata registered the lowest α (0.17) and Ik (838.19 μmol∙m−2∙s−1) values compared to its control plants (α 0.23; Ik 769.99 μmol∙m−2∙s−1). Differences were only obtained in ETRmax in the reproductive stage (50–65 DAE) in which cultivar NUA35 reached values of 158.5 in stressed plants compared to control plants (251.22). In conclusion, the parameters derived from RLCs such as α and Ik can be used as tools to identify drought susceptibility in the vegetative stage, whereas ETRmax can be used in the reproductive stage. In addition, PSII photochemistry (Fv/Fm and NPQ) can also help to understand the agronomic responses of common bush bean cultivars to drought conditions.

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“…Use of the Fr/FFr ratio allowed detection of changes in PSII when plants showed no visual symptoms of toxicity. Therefore, chlorophyll fluorescence is a useful tool for monitoring Pb toxicity in castor bean plants, corroborating results found in studies of Cd toxicity (Silva, Nascimento, Gouveia-Neto, & Silva-Jr., 2012;Marques & Nascimento, 2013;, As toxicity (Stoeva, Berova, & Zlatev, 2004;(Silva et al, 2015), Ni toxicity (Gopal, Mishra, Zeeshan, Prasad, & Joshi, 2002;Mishra & Gopal, 2008), Cu and Hg toxicities (Ventrella, Catucci, Piletska, Piletsky, & Agostiano, 2009), Zn (Cherif et al, 2010;Marques & Nascimento, 2014;Marques et al, 2017) and Pb toxicity (Marques et al, 2017).…”

Section: Chlorophyll Fluorescencementioning

confidence: 99%

“…Aside from investigations of the toxic effects of Pb on plant tissues (visual symptoms; nutritional imbalance; and morphological, metabolic, and physiological disorders), techniques allowing the identification of toxicity or tolerance in early-stage plants are of great importance for monitoring environmental contamination. Chlorophyll fluorescence uses information about the photochemical activity of plants, allowing the early detection of environmental stress (Corcoll, Bonet, Leira, & Guasch 2011, Marques, Nascimento, Silva, Gouveia-Neto, & Silva, 2017. This is possible because the chlorophyll molecule is fluorescent, and through photon dissipation, changes in electron transfer at the level of chloroplast membranes can be detected (Lin, Liu, Lin, Pan, & Peng, 2007).…”

Section: Introductionmentioning

confidence: 99%

<b>Metabolic alterations and X-ray chlorophyll fluorescence for the early detection of lead stress in castor bean (<i>Ricinus communis</i>) plants

Nascimento

Marques

2018

Acta Sci. Agron.

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The remediation of lead-contaminated areas poses a serious challenge to soil chemists because Pb has low solubility in soil. Thus, Pb phytostabilization is considered to be an attractive remediation technique. Castor bean (Ricinus communis) is an oilseed crop known for its tolerance to heavy metals, and our aim was to assess the early detection of Pb toxicity and the effects of Pb on the biomass, photosynthetic pigments, antioxidative enzyme activities, and total soluble proteins of this plant. Specimens were grown in a nutrient solution spiked with Pb concentrations of 25, 50, 100, 150, or 200 µmol L-1. A control without Pb was also grown. The results show that X-ray chlorophyll fluorescence is an efficient technique for the early detection of photosystem II alterations driven by Pb toxicity. Castor bean was tolerant to the Pb doses tested; plants presented no changes in photosynthetic pigments, defense enzyme activities, or total soluble proteins in leaves. Given its ability to tolerate and accumulate Pb in its roots, castor bean is a viable alternative for phytostabilization and phytoattenuation of lead-contaminated areas. It is also economically attractive for industrial and biofuel oil production while being used for remediation.

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Tolerance of an energy crop (Jatropha curcas L.) to zinc and lead assessed by chlorophyll fluorescence and enzyme activity

Cited by 18 publications

References 52 publications

24-Epibrassinolide Improves Root Anatomy and Antioxidant Enzymes in Soybean Plants Subjected to Zinc Stress

24-Epibrassinolide Improves Root Anatomy and Antioxidant Enzymes in Soybean Plants Subjected to Zinc Stress

Chlorophyll α Fluorescence Parameters as an Indicator to Identify Drought Susceptibility in Common Bush Bean

<b>Metabolic alterations and X-ray chlorophyll fluorescence for the early detection of lead stress in castor bean (<i>Ricinus communis</i>) plants

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