Tolerance of an Antarctic Bacterium to Multiple Environmental Stressors

Sengupta, Dipanwita; Sangu, Kavya; Shivaji, Sisinthy; Chattopadhyay, M. K.

doi:10.1007/s00284-015-0874-y

Cited by 2 publications

(2 citation statements)

References 23 publications

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“…Citrate synthase (spot no. 16) is involved in stress tolerance, and its disruption leads to multistress susceptibility in Pseudomonas syringae (43), while its overexpression leads to aluminum tolerance in alfalfa (44), confirming a role in multistress tolerance response. We also reported the implication of cysteine synthase (no.…”

Section: Resultsmentioning

confidence: 85%

Hyperconcentrated Sweet Whey, a New Culture Medium That Enhances Propionibacterium freudenreichii Stress Tolerance

Song

Rabah

Jardin

et al. 2016

Appl Environ Microbiol

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Propionibacterium freudenreichii is used as a cheese-ripening starter and as a probiotic. Its reported physiological effects at the gut level, including modulation of bifidobacteria, colon epithelial cell proliferation and apoptosis, and intestinal inflammation, rely on active metabolism in situ. Survival and activity are thus key factors determining its efficacy, creating stress adaptation and tolerance bottlenecks for probiotic applications. Growth media and growth conditions determine tolerance acquisition. We investigated the possibility of using sweet whey, a dairy by-product, to sustain P. freudenreichii growth. It was used at different concentrations (dry matter) as a culture medium. Using hyperconcentrated sweet whey led to enhanced multistress tolerance acquisition, overexpression of key stress proteins, and accumulation of intracellular storage molecules and compatible solutes, as well as enhanced survival upon spray drying. A simplified process from growth to spray drying of propionibacteria was developed using sweet whey as a 2-in-1 medium to both culture P. freudenreichii and protect it from heat and osmotic injury without harvesting and washing steps. As spray drying is far cheaper and more energy efficient than freeze-drying, this work opens new perspectives for the sustainable development of new starter and probiotic preparations with enhanced robustness. IMPORTANCEIn this study, we demonstrate that sweet whey, a dairy industry by-product, not only allows the growth of probiotic dairy propionibacteria, but also triggers a multitolerance response through osmoadaptation and general stress response. We also show that propionibacteria accumulate compatible solutes under these culture conditions, which might account for the limited loss of viability after spray drying. This work opens new perspectives for more energy-efficient production of dairy starters and probiotics.

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Section: Resultsmentioning

confidence: 85%

Hyperconcentrated Sweet Whey, a New Culture Medium That Enhances Propionibacterium freudenreichii Stress Tolerance

Song

Rabah

Jardin

et al. 2016

Appl Environ Microbiol

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“…Therefore, strong stress resistance of the host organism can help improve bioremediation capacity. The cold-adapted bacteria are generally equipped with diverse stress response systems owing to the fact that the cold environment is a major cause of multiple stresses such as osmotic, alkali, and oxidative stress [ 3 ]. Consequently, particular interest has arisen in regard to the bioremediation ability of psychrotrophs and psychrophiles [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Complete genome sequence of Planococcus sp. PAMC21323 isolated from Antarctica and its metabolic potential to detoxify pollutants

Jung

Joe

Kim

et al. 2018

Stand in Genomic Sci

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The Planococcus sp. PAMC21323 is a yellow pigment-producing bacterium isolated from King George Island in Antarctica; it has a broad growth temperature range of 5–40 °C. Herein, we describe the complete genome sequence information of the genus Planococcus with its annotated sequence, genetic features for bioremediation, and oxidative stress capacity. The Planococcus sp. PAMC21323 possesses chromosomal DNA (3,196,500-bp) with plasmid DNA (3364-bp). The complete 3,199,864-bp of the genome consists of 3171 genes including 60 transfer RNAs and 24 ribosomal RNAs. Strain PAMC21323 encodes various genes associated with detoxification of heavy metal ions and aromatic hydrocarbons. Moreover, it is equipped with diverse stress response systems, which can be used to sense the internal and oxidative stresses caused by detoxification. This is the first report highlighting the genetic potential of Planococcus sp. PAMC21323 in bioremediation, suggesting application of this psychrotrophic strain in bioremediation in harsh environments.Electronic supplementary materialThe online version of this article (10.1186/s40793-018-0334-y) contains supplementary material, which is available to authorized users.

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Tolerance of an Antarctic Bacterium to Multiple Environmental Stressors

Cited by 2 publications

References 23 publications

Hyperconcentrated Sweet Whey, a New Culture Medium That Enhances Propionibacterium freudenreichii Stress Tolerance

Hyperconcentrated Sweet Whey, a New Culture Medium That Enhances Propionibacterium freudenreichii Stress Tolerance

Complete genome sequence of Planococcus sp. PAMC21323 isolated from Antarctica and its metabolic potential to detoxify pollutants

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