Tolerance induction by a single donor pretreatment with the adenovirus vector encoding CTLA4Ig gene in rat orthotopic liver transplantation

Cre/loxP-mediated CTLA4IgG gene transfer induces clinically relevant immunosuppression via on–off gene recombination in vivo

“…Although induction of donor-specific tolerance has been reported utilizing adenovirus coding CTLA4IgG in some experimental transplantation models [16,20,21], skin grafts from both donor type and 3rd-party mouse were rejected within 16 days on recipients in either group III, group IV, or group V, which had accepted cardiac allografts. Furthermore, B cell tolerance was not induced against OVAimmunization under the circumstance of high CTLA4IgG concentration.…”

Modifying graft immunogenicity and immune response prior to transplantation: potential clinical applications of donor and graft treatment

“…Steroids and vitamin D analogs affect all stages of DC maturation and their function. In our own experimental studies, we were able to demonstrate that donor treatment for the induction of HO‐1 was associated with reduced donor‐specific DCs in all recipient compartments [79].…”

Costimulatory blockade prevents early rejection, promotes lymphocyte apoptosis, and inhibits the upregulation of intragraft interleukin-6 in an orthotopic liver transplant model in the rat

“…CLTA4-Ig previously has been shown to prevent liver allograft rejection and induce long-term acceptance in the rat. 9,14,15 To our knowledge, this is the first study to show long-term survival of liver allografts using anti-CD154. The first postoperative week represents a critical time in determining the fate of liver allografts.…”

“…Dosing regimens were based on results from other reports in the literature. 9,[13][14][15] Each transplant recipient was assigned a coded number, and those caring for the animals, measuring outcomes, and analyzing samples were blinded to the treatment group. The Animal Ethics Committee stipulated that surviving animals be killed on or before day 8 if they showed signs of impending liver failure (Ͼ20% loss in weight, reduced activity, or bilirubin Ͼ 200 IU/L).…”