Tolerance and Bioaccumulation of Copper by the Entomopathogen Beauveria bassiana (Bals.-Criv.) Vuill. Exposed to Various Copper-Based Fungicides

Martins, Fátima; Soares, Maria Elisa; Oliveira, Ivo; Pereira, José Alberto; Bastos, Maria de Lourdes; Baptista, Paula

doi:10.1007/s00128-012-0628-5

Cited by 10 publications

(21 citation statements)

References 26 publications

(40 reference statements)

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“…In contrast, mycelial growth was found to be less affected by the fungicide. These results were in agreement with those previously reported for B. bassiana when exposed to non‐copper as well as to copper‐based fungicides, including Bordeaux mixture . Mycelial growth, sporulation and, especially, germination are important in insect infection and disease transmission.…”

Section: Discussionsupporting

confidence: 92%

“…Previous studies by the present authors showed that the addition of Bordeaux mixture to agar medium reduced significantly B. bassiana growth, sporulation and germination . This inhibitory effect was noticed throughout the incubation period (12 days).…”

Section: Introductionsupporting

confidence: 66%

“…These results were in agreement with those previously reported for B. bassiana when exposed to non-copper 3,5 as well as to copperbased fungicides, including Bordeaux mixture. 6 Mycelial growth, sporulation and, especially, germination are important in insect infection and disease transmission. In fact, the first step of the infection process begins with the germination of conidia on the surface of the insect host, and, after penetration, the mycelium begins to grow quickly and intensively, causing the death of the insect.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies by the present authors showed that the addition of Bordeaux mixture to agar medium reduced significantly B. bassiana growth, sporulation and germination. 6 This inhibitory effect was noticed throughout the incubation period (12 days). In order further to understand the mechanism of the toxicity of Bordeaux mixture in B. bassiana, in the present study an examination was made of the effect of this fungicide on mycelial growth and conidium production and germination, as well as their relation with the antioxidant defence system on fungal submerged cultures within the first 24 h of elicitation.…”

Section: Introductionmentioning

confidence: 93%

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Oxidative stress response of Beauveria bassiana to Bordeaux mixture and its influence on fungus growth and development

Martins

Pereira

Baptista

2013

Pest Management Science

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Section: Discussionsupporting

confidence: 92%

Section: Introductionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

See 2 more Smart Citations

Oxidative stress response of Beauveria bassiana to Bordeaux mixture and its influence on fungus growth and development

Martins

Pereira

Baptista

2013

Pest Management Science

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“…Another concern in applying algicides to treat CyanoHABs is leaving harmful chemical residuals that cause secondary contamination (65). CuSO 4 is not only an algicide but also a fungicide and insecticide (66,67). Similarly, the function of tryptamine and tryptoline is much broader than lysing cyanobacteria.…”

Section: Discussionmentioning

confidence: 99%

Algicidal Activity of Streptomyces eurocidicus JXJ-0089 Metabolites and Their Effects on Microcystis Physiology

Zhang

Ding

et al. 2016

Appl Environ Microbiol

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Copper sulfate (CuSO 4 ) has been widely used as an algicide to control harmful cyanobacterial blooms (CyanoHABs) in freshwater lakes. However, there are increasing concerns about this application, due mainly to the general toxicity of CuSO 4 to other aquatic species and its long-term persistence in the environment. This study reported the isolation and characterization of two natural algicidal compounds, i.e., tryptamine and tryptoline, from Streptomyces eurocidicus JXJ-0089. At a concentration of 5 g/ml, both compounds showed higher algicidal efficiencies than CuSO 4 on Microcystis sp. FACHB-905 and some other harmful cyanobacterial strains. Tryptamine and tryptoline treatments induced a degradation of chlorophyll and cell walls of cyanobacteria. These two compounds also significantly increased the intracellular oxidant content, i.e., superoxide anion radical (O 2 ؊ ) and malondialdehyde (MDA), but reduced the activity of intracellular reductants, i.e., superoxide dismutase (SOD), of cyanobacteria. Moreover, tryptamine and tryptoline treatments significantly altered the internal and external contents of microcystin-LR (MC-LR), a common cyanotoxin. Like CuSO 4 , tryptamine and tryptoline led to releases of intracellular MC-LR from Microcystis, but with lower rates than CuSO 4 . Tryptamine and tryptoline (5 g/ml) in cyanobacterial cultures were completely degraded within 8 days, while CuSO 4 persisted for months. Overall, our results suggest that tryptamine and tryptoline could potentially serve as more efficient and environmentally friendly alternative algicides than CuSO 4 in controlling harmful cyanobacterial blooms. IMPORTANCECyanobacterial harmful algal blooms (CyanoHABs) in aquatic environments have become a worldwide problem. Numerous efforts have been made to seek means to prevent, control, and mitigate CyanoHABs. Copper sulfate (CuSO 4 ), was once a common algicide to treat and control CyanoHABs. However, its application has become limited due to concerns about its general toxicity to other aquatic species and its long-term persistence in the environment. There is a great need for algicides with higher specificity and low environmental impacts. This study reports the isolation and characterization of two natural algicidal compounds from a streptomycete strain, Streptomyces eurocidicus JXJ-0089. Our results suggest that the identified algicides could potentially serve as more efficient and environmentally friendly alternative algicides than CuSO 4 in controlling harmful cyanobacterial blooms. Harmful cyanobacterial blooms (CyanoHABs) have become a global phenomenon and are occurring with increasing intensity, area of infection, and frequency (1). One danger that CyanoHABs have posed to the environment and human health is the production and release of cyanotoxins. The most frequently detected cyanotoxins in freshwater systems are microcystins (MCs), a group of potent liver toxins (2, 3). An MC contamination incident in 1996 caused the death of 53 patients in Caruaru, Brazil (4). A more recent MC c...

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Towards more Ecofriendly Pesticides: Use of Biosurfactants Obtained from the Corn Milling Industry as Solubilizing Agent of Copper Oxychloride

López-Prieto

Moldes

Cruz

et al. 2020

J Surfact & Detergents

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Copper oxychloride (Cu-Oxy) is a widely used copper-based fungicide. A drawback of the product is its very low water solubility (around 0.2%) which limits its fungicide efficiency due to the low-penetrating capacity in the fungal mycelia. In this study, we have explored the use of biosurfactants (BS), surface-active compounds obtained by microbial production, to enhance the solubility of Cu-Oxy in agrochemical formulations. A BS extracted from corn steep liquor (CSL), a residue from the corn-milling industry, was evaluated as an additive to increase the solubility of Cu-Oxy in water. Different types of synthetic surfactants (Tween ® 80, sodium dodecyl sulfate (SDS) and hexadecyltrimethylammonium bromide (CTAB)) were included for comparison. Results showed that it was possible to dissolve around 90% of Cu-Oxy at concentration of 4 g L −1 in 20 min of contact time when the BS was added at the concentration of 20 g L −1. In contrast, the synthetic surfactants were only able to dissolve less than 0.5% of Cu-Oxy under the same conditions. In addition, a factorial design was carried out in order to evaluate the influence of BS concentration, Cu-Oxy concentration and contact time, on the solubilization of Cu-Oxy. Maximum percentage of solubilization (96.5%) was achieved at concentrations of 16.1 g L −1 of BS, 2 g L −1 of Cu-Oxy, and 20 min of contact time. These results allowed to conclude that BS extracted from CSL could be included in agrochemical formulations as solubilizing agents improving the stability of hydrophobic active principles and reducing the concentration needed of chemical pesticides, giving eco-friendlier formulations.

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Tolerance and Bioaccumulation of Copper by the Entomopathogen Beauveria bassiana (Bals.-Criv.) Vuill. Exposed to Various Copper-Based Fungicides

Cited by 10 publications

References 26 publications

Oxidative stress response of Beauveria bassiana to Bordeaux mixture and its influence on fungus growth and development

Oxidative stress response of Beauveria bassiana to Bordeaux mixture and its influence on fungus growth and development

Algicidal Activity of Streptomyces eurocidicus JXJ-0089 Metabolites and Their Effects on Microcystis Physiology

Towards more Ecofriendly Pesticides: Use of Biosurfactants Obtained from the Corn Milling Industry as Solubilizing Agent of Copper Oxychloride

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