Marcel G. Wubbolts, Peter Reuvekamp, Bernard Witholt
Abstract:Xylene oxygenase, which is encoded on the TOL plasmid pWWO of Pseudomonas putida mt-2, is a key enzyme system in the degradation of toluene and xylenes by this organism. It was expressed in an Escherichia coli recombinant strain carrying the xylMA structural genes. This recombinant, which expressed xylene oxygenase from the heat-shock induced lambda PL promoter, was analyzed for its potential as a biocatalytic tool so as to effect the oxidation of side chains of aromatic hydrocarbons to the corresponding alcoh…
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