Toehold of dsDNA exchange affects the hydrogel swelling kinetics of a polymer–dsDNA hybrid hydrogel

Gao, Ming; Gawel, Kamila; Stokke, Bjørn Torger

doi:10.1039/c0sm00915f

Cited by 44 publications

(39 citation statements)

References 67 publications

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“…If the linker DNA did not have any initial secondary structure, gel shrinkage upon hybridization to the cDNA was observed [65]. The change of the gel volume is quite small (only ~20%) [66,67]. To increase the sensitivity of detection, interferometry and gel photonic crystals have been reported [68,69].…”

Section: Sensors Based On Gel Volume Changementioning

confidence: 99%

“…Another way to control gel swelling is shown in Figure 3B, where the gel is mainly crosslinked by conventional bis-acrylamide. Hybridization of DNA establishes more crosslinking points and thus shrinks the gel [66]. In a detailed study, Gao et al tuned gel swelling kinetics by changing the length of cDNA that would compete with the linker DNA [66].…”

Section: Sensors Based On Gel Volume Changementioning

confidence: 99%

“…Hybridization of DNA establishes more crosslinking points and thus shrinks the gel [66]. In a detailed study, Gao et al tuned gel swelling kinetics by changing the length of cDNA that would compete with the linker DNA [66]. At the same time, increasing the crosslink density decreases the swelling rate, whereas destabilization of the linker at a temperature close to the DNA melting point increases the swelling rate.…”

Section: Sensors Based On Gel Volume Changementioning

confidence: 99%

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Visual optical biosensors based on DNA-functionalized polyacrylamide hydrogels

Khimji

Kelly

Helwa

et al. 2013

Methods

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Section: Sensors Based On Gel Volume Changementioning

confidence: 99%

Section: Sensors Based On Gel Volume Changementioning

confidence: 99%

Section: Sensors Based On Gel Volume Changementioning

confidence: 99%

See 1 more Smart Citation

Visual optical biosensors based on DNA-functionalized polyacrylamide hydrogels

Khimji

Kelly

Helwa

et al. 2013

Methods

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“…It has already proved its potential in sensing such analytes as glucose [2,3] and oligonucleotides [4][5][6]. Herein we present the technique as a useful tool for label-free monitoring of swelling phenomena occurring in various hydrogelamphiphile and hydrogel-amphiphile-macrocycle cases.…”

Section: Introductionmentioning

confidence: 99%

High resolution interferometry as a tool for characterization of swelling of weakly charged hydrogels subjected to amphiphile and cyclodextrin exposure

Gao

Gawel

Stokke

2013

Journal of Colloid and Interface Science

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A high resolution interferometric technique was used to determine swelling behavior of weakly charged polyacrylamide hydrogels in the presence of oppositely charged surfactants and subsequent exposure to cyclodextrins. Hydrogels of copolymerized acrylamide and 2-acrylamido-2-methyl-1-propanesulfonic acid (0.22, 0.44, 0.88 mol%) and crosslinked with bisacrylamide (3, 6, 12 mol%) were employed. The equilibrium swelling and swelling kinetics of the hydrogels were determined with 2 nanometer resolution of the optical length and sampled at approximately 1 Hz. These properties were determined for the hydrogels exposed to cationic surfactants dodecyltrimethylammonium bromide (DTAB) and cetyltrimethylammonium bromide (CTAB) at concentrations from 10

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“…If complementary ssDNA oligonucleotides are applied to the gel, they spontaneously hybridize to the crosslinks and change the overall conformation of the gel. This can alter the gel's stiffness [64], volume [65,66], or induce its dissolution [67].…”

Section: Application Area 2: Production Of Improved Scaffolds For Tismentioning

confidence: 99%

Application of Synthetic Biology to Regenerative Medicine

Cachat¹,

Davies²

2011

J Bioengineer & Biomedical Sci

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Synthetic biology uses interchangeable and standardized "bio-parts" to construct complex genetic networks that include sensing, information processing and effector modules: these allow robust and tunable transgene expression in response to a change in signal input. The rise of this field has coincided closely with the emergence of regenerative medicine as a distinct discipline. Unlike synthetic biology, regenerative medicine uses the natural abilities of cells to make trophic factors and to produce new tissues as they would in normal development and tissue maintenance. In this article, we argue that bringing these young fields together, so that synthetic biology techniques are applied to the problem of regeneration, has the potential significantly to enhance our ability to help those in clinical need. We first review the synthetic tool kit available for engineered mammalian networks, then examine the main areas in which synthetic biology techniques might be applied to promote regeneration: (i) biosynthesis and controlled release of therapeutic molecules, (ii) synthesis of scaffold material, (iii) regulation of stem cells, and (iv) programming cells to organize themselves into novel tissues. We finally consider the long-term potential of synthetic biology for regenerative medicine, and the risks and challenges ahead.

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Toehold of dsDNA exchange affects the hydrogel swelling kinetics of a polymer–dsDNA hybrid hydrogel

Cited by 44 publications

References 67 publications

Visual optical biosensors based on DNA-functionalized polyacrylamide hydrogels

Visual optical biosensors based on DNA-functionalized polyacrylamide hydrogels

High resolution interferometry as a tool for characterization of swelling of weakly charged hydrogels subjected to amphiphile and cyclodextrin exposure

Application of Synthetic Biology to Regenerative Medicine

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