Tocotrienol-Rich Fraction Prevents Cell Cycle Arrest and Elongates Telomere Length in Senescent Human Diploid Fibroblasts

Makpol, Suzana; Durani, Lina Wati; Chua, Kien Hui; Yusof, Yasmin Anum Mohd; Ngah, Wan Zurinah Wan

doi:10.1155/2011/506171

Cited by 53 publications

(55 citation statements)

References 39 publications

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“…Herein, we can propose that H 2 O 2 induced senescence in NIH3T3 cells through the initiation of OS, ROS formation, and overexpression of inflammatory cytokines. 56,57 In molecular viewpoint, the accumulation of OS (in this case H 2 O 2 ) induces p53-dependent apoptosis, resulting in cells passage to G1 and undergo arrest. Furthermore, OS activates cellular inflammatory responses that are generally controlled by the transcription factor NF-κB.…”

Section: Discussionmentioning

confidence: 99%

Manipulation of molecular pathways and senescence hallmarks by natural compounds in fibroblast cells

Momtaz

Baeeri

Rahimifard

et al. 2018

J of Cellular Biochemistry

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Aging contributes to an increased risk of developing a number of neurodegenerative and chronic disorders, predominantly related to oxidative stress (OS) and defects in the antioxidant balance. This study focused on the antisenescence effect of four plant species (Falcaria vulgaris, Ixiolirion tataricum, Ajuga chamaecistus, and Scabiosa flavida) on H2O2‐induced premature senescence in rat NIH3T3 fibroblasts, which were found to be rich in effective phytochemicals with traditional ethnobotanical backgrounds. Plant materials were collected, identified, and extracted. To determine the viability of NIH3T3 cells, an MTT assay was conducted. The levels of OS markers and the senescence‐associated ß‐galactosidase (SA‐ß‐GAL) activity were analyzed by the Elisa reader. The cell cycle pattern was evaluated by flow cytometry. The expression of senescence‐related inflammatory cytokines and the molecules involved in aging signaling pathways were investigated using the real‐time reverse transcription polymerase chain reaction (RT‐PCR). H2O2 treatment decreased cell viability and increased lipid peroxidation (LPO) and the reactive oxygen species (ROS) in NIH3T3s. However, S. flavida exhibited low cytotoxicity, reduced OS and SA‐ß‐GAL activities in NIH3T3 cells compared with the H2O2‐treated group. I. tataricum was the second best plant, although it was more toxic to NIHT3T cells. S. flavida decreased G0/G1 arrest and facilitated the G2/M transition of NIH3T3s, also downregulated the expression of p38, p53, p16, and the related inflammatory mediators. S. flavida potentially modulated senescence‐associated hallmarks in fibroblasts exposed to H2O2, thus it may inhibit the aging process via controlling the OS. Therefore it is a promising candidate for future antiaging explorations.

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Section: Discussionmentioning

confidence: 99%

Manipulation of molecular pathways and senescence hallmarks by natural compounds in fibroblast cells

Momtaz

Baeeri

Rahimifard

et al. 2018

J of Cellular Biochemistry

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“…Numerous studies suggest that antioxidants can prevent the oxidation of various macromolecules, such as DNA, proteins, and lipids, thus preventing the aging process and increasing the lifespan of the organism (13,14). In this study, we investigated three types of antioxidants: Piper betle (PB), the tocotrienol-rich fraction (TRF), and Chlorella vulgaris (CV).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant enzyme activity and malondialdehyde levels can be modulated by Piper betle, tocotrienol rich fraction and Chlorella vulgaris in aging C57BL/6 mice

Aliahmat¹,

Noor²,

Yusof³

et al. 2012

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OBJECTIVE:The aim of this study was to determine the erythrocyte antioxidant enzyme activity and the superoxide dismutase, catalase, glutathione peroxidase, and plasma malondialdehyde levels in aging mice and to evaluate how these measures are modulated by potential antioxidants, including the tocotrienol-rich fraction, Piper betle, and Chlorella vulgaris.METHOD:One hundred and twenty male C57BL/6 inbred mice were divided into three age groups: young (6 months old), middle-aged (12 months old), and old (18 months old). Each age group consisted of two control groups (distilled water and olive oil) and three treatment groups: Piper betle (50 mg/kg body weight), tocotrienol-rich fraction (30 mg/kg), and Chlorella vulgaris (50 mg/kg). The duration of treatment for all three age groups was two months. Blood was withdrawn from the orbital sinus to determine the antioxidant enzyme activity and the malondialdehyde level.RESULTS:Piper betle increased the activities of catalase, glutathione peroxidase, and superoxide dismutase in the young, middle, and old age groups, respectively, when compared to control. The tocotrienol-rich fraction decreased the superoxide dismutase activity in the middle and the old age groups but had no effect on catalase or glutathione peroxidase activity for all age groups. Chlorella vulgaris had no effect on superoxide dismutase activity for all age groups but increased glutathione peroxidase and decreased catalase activity in the middle and the young age groups, respectively. Chlorella vulgaris reduced lipid peroxidation (malondialdehyde levels) in all age groups, but no significant changes were observed with the tocotrienol-rich fraction and the Piper betle treatments.CONCLUSION:We found equivocal age-related changes in erythrocyte antioxidant enzyme activity when mice were treated with Piper betle, the tocotrienol-rich fraction, and Chlorella vulgaris. However, Piper betle treatment showed increased antioxidant enzymes activity during aging.

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“…Tocopherols are saturated forms of vitamin E, and tocotrienols are the unsaturated forms, distinguishable by the three double bonds in the tails of tocotrienols (7). Studies showed that tocotrienol is superior for its antioxidant properties as well as increasing cells viability and proliferation (8,9). The current project aim to evaluate the response of human periodontal ligament fibroblasts (HPdLF) upon exposure to various concentrations of tocotrienols rich fraction (TRF) conditioned medium.…”

Section: Introductionmentioning

confidence: 99%

Effect of vitamin E on human periodontal ligament fibroblasts

Maurtgi¹,

Mohamad²,

Noor³

et al. 2016

AIP Conference Proceedings

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Tocotrienol-Rich Fraction Prevents Cell Cycle Arrest and Elongates Telomere Length in Senescent Human Diploid Fibroblasts

Cited by 53 publications

References 39 publications

Manipulation of molecular pathways and senescence hallmarks by natural compounds in fibroblast cells

Manipulation of molecular pathways and senescence hallmarks by natural compounds in fibroblast cells

Antioxidant enzyme activity and malondialdehyde levels can be modulated by Piper betle, tocotrienol rich fraction and Chlorella vulgaris in aging C57BL/6 mice

Effect of vitamin E on human periodontal ligament fibroblasts

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