Toc12, a Novel Subunit of the Intermembrane Space Preprotein Translocon of Chloroplasts

Becker, Thomas; Hritz, Jozef; Vogel, Markus; Caliebe, Alexander; Bukau, Bernd; Soll, Jiirgen; Schleiff, Enrico

doi:10.1091/mbc.e04-05-0405

Cited by 91 publications

(112 citation statements)

References 74 publications

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“…In other cases, the Hsp70 and type III J-domain protein are recruited independently to the site of action, where the J-domain protein stimulates ATP hydrolysis by the partner Hsp70. This can be observed for the import machinery of mitochondria and chloroplasts (Westermann and Neupert, 1997;Becker et al, 2004;Kozany et al, 2004;Qbadou et al, 2007). The divergence of the C terminus among the type III J-domain proteins and their uniqueness suggests the possibility that they bind very different proteins.…”

Section: Discussionmentioning

confidence: 93%

OWL1: An Arabidopsis J-Domain Protein Involved in Perception of Very Low Light Fluences

Kneissl¹,

Wachtler

Chua

et al. 2009

Plant Cell

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Section: Discussionmentioning

confidence: 93%

OWL1: An Arabidopsis J-Domain Protein Involved in Perception of Very Low Light Fluences

Kneissl¹,

Wachtler

Chua

et al. 2009

Plant Cell

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“…Tic21 was recently identified by Arabidopsis genetics (Teng et al, 2006). When antibodies against pea Tic110 and SPA-820 monoclonal antibody (StressGen), which recognizes an as yet unidentified intermembrane space Hsp70 (Schnell et al, 1994;Becker et al, 2004), were added, the mobility of the 1-MD complex on BN-PAGE was not affected ( Figure 5A). All other antibodies, which we tested, against known translocon components failed to shift the 1-MD complex by antibody-shift BN-PAGE (i.e., antibodies against Toc159, Toc34, Tic40, Tic22, Cpn60a, Cpn60b, and stromal Hsp70) (see Supplemental Figure 6 online).…”

Section: Tic21 Is Involved In the 1-md Translocation Complexmentioning

confidence: 98%

A 1-Megadalton Translocation Complex Containing Tic20 and Tic21 Mediates Chloroplast Protein Import at the Inner Envelope Membrane

et al. 2009

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Chloroplast protein import is mediated by two hetero-oligomeric protein complexes, the Tic and Toc translocons, which are located in the inner and outer envelope membranes. At the inner membrane, many Tic components have been identified and characterized, but it remains unclear how these Tic proteins are organized to form a protein-conducting channel or whether a stable Tic core complex that binds translocating preproteins exists. Here, we report the identification of a 1-megadalton (MD) translocation complex as an intermediate during protein translocation across the inner membrane in Arabidopsis thaliana and pea (Pisum sativum). This complex can be detected by blue native PAGE using the mild detergent digitonin without any chemical cross-linkers. The preprotein arrested in the 1-MD complex can be chased into its fully translocated form after a subsequent incubation. While Tic20 and Tic21 appear to be involved in the 1-MD complex, Tic110, a wellcharacterized Tic component, exists as a distinct entity from the complex. Several lines of evidence suggest that the 1-MD complex functions in between the Toc and Tic110-containing complexes, most likely as a protein-conducting channel at the inner envelope.

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“…The existence of Toc12 in IMS, however, has been controversially discussed in the recent years (Becker et al , 2004a ;Chen et al , 2010 ;Chiu et al , 2010 ). Toc12 was annotated as a J-domain protein named AtJ8 in Arabidopsis and as a truncated clone of pea PsJ8b Chiu et al , 2010 ).…”

Section: The Molecular Framework Of the Toc Complexmentioning

confidence: 99%

The gateway to chloroplast: re-defining the function of chloroplast receptor proteins

Chang¹,

Soll²,

Bölter³

2012

Biological Chemistry

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: Chloroplast biogenesis often requires a tight orchestration between gene expression (both plastidial and nuclear) and translocation of ~ 3000 nuclear-encoded proteins into the organelle. Protein translocation is achieved via two multimeric import machineries at the outer (TOC) and inner (TIC) envelope of chloroplast, respectively. Three components constitute the core element of the TOC complex: a β -barrel protein translocation channel Toc75 and two receptor constituents, Toc159 and Toc34. A diverse set of distinct TOC complexes have recently been characterized and these diversified TOC complexes have evolved to coordinate the translocation of differentially expressed proteins. This review aims to describe the recent discoveries relating to the typical characteristics of these distinct TOC complexes, particularly the receptor constituents, which are the main contributors for TOC complex diversification.

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Toc12, a Novel Subunit of the Intermembrane Space Preprotein Translocon of Chloroplasts

Cited by 91 publications

References 74 publications

OWL1: An Arabidopsis J-Domain Protein Involved in Perception of Very Low Light Fluences

OWL1: An Arabidopsis J-Domain Protein Involved in Perception of Very Low Light Fluences

A 1-Megadalton Translocation Complex Containing Tic20 and Tic21 Mediates Chloroplast Protein Import at the Inner Envelope Membrane

The gateway to chloroplast: re-defining the function of chloroplast receptor proteins

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