Tobacco transcription repressors NtJAZ: Potential involvement in abiotic stress response and glandular trichome induction

Zhang, Hongying; Li, Wenjiao; Niu, Dexin; Wang, Zhaojun; Yan, Xiaoxiao; Yang, Xiuhong; Yang, Yuzhu

doi:10.1016/j.plaphy.2019.06.021

Cited by 22 publications

(24 citation statements)

References 46 publications

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“…JAZ repressors have been universally reported to participate in extensive plant growth activities and stress responses and to play a hub role in hormonal crosstalk between SA-JA, ETH-JA and AUX-JA 7 . Eighteen JAZs have been found in apples 26 , 7 in sugarcane 27 , 18 in the rubber tree 28 , 15 in tobacco 29 and 13 in tomatoes. However, the functional role of JAZ genes in the tea plant is poorly understood.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide and expression pattern analysis of JAZ family involved in stress responses and postharvest processing treatments in Camellia sinensis

Chen

Wang

Sun

et al. 2020

Sci Rep

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the JASMONATE-ZIM DOMAIN (JAZ) family genes are key repressors in the jasmonic acid signal transduction pathway. Recently, the JAZ gene family has been systematically characterized in many plants. However, this gene family has not been explored in the tea plant. In this study, 13 CsJAZ genes were identified in the tea plant genome. Phylogenetic analysis showed that the JAZ proteins from tea and other plants clustered into 11 sub-groups. The CsJAZ gene transcriptional regulatory network predictive and expression pattern analyses suggest that these genes play vital roles in abiotic stress responses, phytohormone crosstalk and growth and development of the tea plant. in addition, the CsJAZ gene expression profiles were associated with tea postharvest processing. Our work provides a comprehensive understanding of the CsJAZ family and will help elucidate their contributions to tea quality during tea postharvest processing.Higher plants face a large number of severe challenges during their life cycles, including insect bites, pathogen infection, heavy metal stress, and water scarcity. However, as sessile organisms, plants have evolved sophisticated mechanisms to resist these problems and clever strategies to thrive in their ever-changing natural environments 1,2 . For plants, phytohormones are the most effective and fastest weapon in response to environmental stress. For example, jasmonic acid (JA) is widely known to play an important role in various biological processes in plants, including defense against herbivorous insect attack, flower initiation and plant morphogenesis [3][4][5] .There are at least two jasmonate synthesis pathways that exist in plants, namely octadecane pathway and hexadecanoid pathway, which begins with the release of α-linolenic acid (18:3n-3) and hexadecatrienoic acid (16:3n-3), respectively 6 . The unsaturated fatty acids are catalyzed by a series of enzymes then generates 12-oxo-10,15 (Z)-phytodienoic acid (OPDA), in the chloroplast 7 . Finally, JA is formed through OPDA reductase 3 (OPR3)-mediated reduction reaction and three rounds of β-oxidation 6 . Interestingly, an alternative pathway for JA biosynthesis was discovered. OPDA could enter the β-oxidation pathway to produce a direct precursor of JA and JA-lie in the absence of OPR3 8 . Moreover, the JA signal pathway has been deciphered 9,10 . It has been reported that the JA receptor is a co-receptor complex formed by JAZ protein, COI1 (CORONATINE INSENSITIVE1) protein and inositol pentakisphosphate 11 . Previous studies have revealed that the JA content is maintained at a relatively low level in plants under normal conditions, in which the JAZ repressor interacts with MYC2 to inhibit downstream insect-resistant or disease-resistant gene expression 12 . However, large amounts of JA accumulate in plant cells in response to abiotic or biotic stresses and are perceived by COI1 13,14 . Subsequently, JAZ proteins are degraded by COI1-mediated E3 ubiquitination. Then, the transcription activator MYC is relieved, which increases the expression o...

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Section: Discussionmentioning

confidence: 99%

Genome-wide and expression pattern analysis of JAZ family involved in stress responses and postharvest processing treatments in Camellia sinensis

Chen

Wang

Sun

et al. 2020

Sci Rep

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“…Bioinformatics analysis revealed that the PatJAZ6 gene showed high homology with 12 JAZs from A. thaliana Arabidopsis and contained highly conserved ZIM and Jas domains, indicating that PatJAZ6 may have similar effects to previously reported JAZ proteins. The expression profiles under MeJA revealed that 300 µM MeJA was the lowest effective concentration to detect the expression of PatJAZ6 , this concentration is higher in comparison with other JAZs, such as NtJAZ in tobacco [29] and SmJAZ in Salvia miltiorrhiza [30], that response to 100 µM MeJA. Subcellular localization results showed that PatJAZ6 was localized in the nucleus (Figure 2).…”

Section: Discussionmentioning

confidence: 99%

PatJAZ6 Acts as a Repressor Regulating JA-Induced Biosynthesis of Patchouli Alcohol in Pogostemon Cablin

Wang

Chen

Zhong

et al. 2019

IJMS

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The JASMONATE ZIM DOMAIN (JAZ) proteins act as negative regulators in the jasmonic acid (JA) signaling pathways of plants, and these proteins have been reported to play key roles in plant secondary metabolism mediated by JA. In this study, we firstly isolated one JAZ from P. cablin, PatJAZ6, which was characterized and revealed based on multiple alignments and a phylogenic tree analysis. The result of subcellular localization indicated that the PatJAZ6 protein was located in the nucleus of plant protoplasts. The expression level of PatJAZ6 was significantly induced by the methyl jasmonate (MeJA). Furthermore, by means of yeast two-hybrid screening, we identified two transcription factors that interact with the PatJAZ6, the PatMYC2b1 and PatMYC2b2. Virus-induced gene silencing (VIGS) of PatJAZ6 caused a decrease in expression abundance, resulting in a significant increase in the accumulation of patchouli alcohol. Moreover, we overexpressed PatJAZ6 in P. cablin, which down-regulated the patchoulol synthase expression, and then suppressed the biosynthesis of patchouli alcohol. The results demonstrate that PatJAZ6 probably acts as a repressor in the regulation of patchouli alcohol biosynthesis, contributed to a model proposed for the potential JA signaling pathway in P. cablin.

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“…NtJAZ1 and NtJAZ3 are two repressors of nicotine biosynthesis (Shoji and Hashimoto, 2011b;Shoji et al, 2008;Zhang et al, 2012). In recent, NtJAZs were mined from the tobacco genomes, showing that NtJAZ7, and NtJAZ10 were also associated with the regulation of nicotine biosynthesis (Zhang et al, 2019). We identified the promoter sequences of 13 NtJAZs from the genome of NC90 tobacco curated at NCBI and named these as NtJAZ1pro through NtJAZ13pro.…”

Section: Mre and G-box Elements Identified In The Promoters Of Ntjaz1mentioning

confidence: 99%

“…When roots lack the bioactive isoleucine-JA (JA-Ile), two N. tabacum JAZ repressors, NtJAZ1 and NtJAZ3, bind to NtMYC2 to turn off the nicotine biosynthesis. In addition, a recent study revealed additional 13 NtJAZs, in which NtJAZ7 and 10 were shown to associate with the regulation of the nicotine biosynthesis (Zhang et al, 2019). When JA-Ile exists, it induces the interaction of SCF COI1 and JAZ to form a complex, which leads to the ubiquitination of JAZs to release NtMYC2.…”

Section: Introductionmentioning

confidence: 99%

A De Novo regulation design shows an effectiveness in altering plant secondary metabolism

Hovary

et al. 2020

Preprint

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Decrease of nicotine, other tobacco alkaloids (OTAs), and carcinogenic tobacco specific nitrosamines (TSNAs) is important for health. To reduce these compounds, we selected MRE and G-box elements of promoters of four Nicotiana tabacum Jasmonate ZIM-domain (NtJAZ) repressor genes as targets to screen transcription factors (TFs) and then identified Arabidopsis Production of Anthocyanin Pigment 1 (PAP1) and Transparent Testa 8 (TT8) for a novel regulation design. Electrophoretic mobility shift, cross-linked chromatin immunoprecipitation, and dual luciferase analyses demonstrated that PAP1, TT8, PAP1/TT8, and the PAP1/TT8/Transparent Testa Glabra 1 (TTG1) complex bound to and activated NtJAZ promoters. Stacked PAP1 (P) and TT8 (T), which were introduced to commercial tobacco cultivars, Narrow Leaf Madole (NL) and KY171 (KY), upregulated four NtJAZs, downregulated seven/eight nicotine biosynthetic genes, and reduced nicotine and nornicotine in transgenic red P+T-NL and P+L-KY plants. Field trials of red P+T-NL, P+L-KY, and PAP1 tobacco genotypes and metabolic quantification showed significant reduction of nicotine and four OTAs in most or all cured leaves. Three TSNAs and the fourth one were diminished by 63-92% and 30-74% in contents in all leaves of P+T-NL and P+L-KY genotypes, respectively. The total content of each TSNA was significantly reduced by 25-60% in leaves of PAP1 plants. Taken together, this regulation designed crossing from Arabidopsis anthocyanin to tobacco alkaloid pathway is defined as a distant pathway-cross regulation (DPCR). The proof of concept of DPCR unearths new functions of two known TFs, new activators of NtJAZs, and a negative regulation pathway of nicotine biosynthesis in red tobacco.

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Tobacco transcription repressors NtJAZ: Potential involvement in abiotic stress response and glandular trichome induction

Cited by 22 publications

References 46 publications

Genome-wide and expression pattern analysis of JAZ family involved in stress responses and postharvest processing treatments in Camellia sinensis

Genome-wide and expression pattern analysis of JAZ family involved in stress responses and postharvest processing treatments in Camellia sinensis

PatJAZ6 Acts as a Repressor Regulating JA-Induced Biosynthesis of Patchouli Alcohol in Pogostemon Cablin

A De Novo regulation design shows an effectiveness in altering plant secondary metabolism

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