Tobacco Mosaic Virus Infection of Chrysanthemums in Thailand: Development of Colorimetric Reverse-Transcription Loop-Mediated Isothermal Amplification (RT–LAMP) Technique for Sensitive and Rapid Detection

Supakitthanakorn, Salit; Vichittragoontavorn, Kanjana; Sunpapao, Anurag; Kunasakdakul, Kaewalin; Thapanapongworakul, Pilunthana; Ruangwong, On‐Uma

doi:10.3390/plants11141788

Cited by 8 publications

(8 citation statements)

References 38 publications

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“…The final results showed that ‘Jianyunxiang’, ‘Quanxiang Wansheng’ and ‘Quanxiang Chongtian’ were only infected with CVB, and ‘Chixian Jingou’ was only infected with TAV and CVB [ 85 ]. Supakitthanakorn et al ground the chrysanthemum leaf that tested positive for TMV with 0.1 M phosphate buffer and used it to mechanically inoculate carborundum-dusted chrysanthemum leaves, confirming the pathogenicity of TMV in chrysanthemums [ 86 ].…”

Section: Research Progress In Chrysanthemum Virus Detection Technologymentioning

confidence: 99%

Current Achievements and Future Prospects in Virus Elimination Technology for Functional Chrysanthemum

Gao,

Chen,

Pan

et al. 2023

Viruses

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Chrysanthemum is an important functional plant that is used for food, medicine and tea. Functional chrysanthemums become infected with viruses all around the world, seriously lowering their quality and yield. Viral infection has become an important limiting factor in chrysanthemum production. Functional chrysanthemum is often propagated asexually by cutting during production, and viral infection of seedlings is becoming increasingly serious. Chrysanthemums can be infected by a variety of viruses causing different symptoms. With the development of biotechnology, virus detection and virus-free technologies for chrysanthemum seedlings are becoming increasingly effective. In this study, the common virus species, virus detection methods and virus-free technology of chrysanthemum infection are reviewed to provide a theoretical basis for virus prevention, treatment and elimination in functional chrysanthemum.

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Section: Research Progress In Chrysanthemum Virus Detection Technologymentioning

confidence: 99%

Current Achievements and Future Prospects in Virus Elimination Technology for Functional Chrysanthemum

Gao,

Chen,

Pan

et al. 2023

Viruses

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“…To determine possible cross reactivity to other viruses and viroids, the positive cDNA of other viruses and viroids including cucumber mosaic virus (CMV), chrysanthemum virus B (CVB), tobacco mosaic virus (TMV), turnip mosaic virus (TuMV) and chrysanthemum chlorotic mottle viroid (CChMVd) were examined. CMV was isolated from tobacco while CVB, TMV, TuMV and CChMVd were isolated from chrysanthemum (Supakitthanakorn et al 2022a;Supakitthanakorn et al 2022b). Total RNA of all viruses and CChMVd were isolated from infected leaves by using TRIzol ® Reagent (Invitrogen, Waltham, USA) according to the manufacturer's instructions and then used as templates for cDNA synthesis by using ReverTra Ace TM qPCR Master Mix with gDNA Remover (Toyobo, Osaka, Japan) according to the manufacturer's instructions.…”

Section: Specificity Assaymentioning

confidence: 99%

“…Moreover, to reduce the risk of contamination and enhance the rapidness, one step RT-LAMP was developed to detect pepino mosaic virus (PepMV) from tomato (Hasiów-Jaroszewska and Borodynko 2013). Recently, LAMP has been widely used to detect plant pathogens, especially viruses and viroids (Panno et al 2020), such as, tobacco mosaic virus (TMV) (Supakitthanakorn et al 2022b), maize chlorotic mottle virus (MCMV) (Liu et al 2016), mirafiori lettuce big vein virus (MiLBVV) (Almasi 2017), columnea latent viroid (CLVd) (Bhuvitarkorn et al 2019) and plum viroid I (PVdI) (Bester and Maree 2022). To avoid post-amplification contamination, the colorimetric LAMP has been applied to detect viruses and viroids (Bhuvitarkorn et al 2019;Supakitthanakorn et al 2022b).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, LAMP has been widely used to detect plant pathogens, especially viruses and viroids (Panno et al 2020), such as, tobacco mosaic virus (TMV) (Supakitthanakorn et al 2022b), maize chlorotic mottle virus (MCMV) (Liu et al 2016), mirafiori lettuce big vein virus (MiLBVV) (Almasi 2017), columnea latent viroid (CLVd) (Bhuvitarkorn et al 2019) and plum viroid I (PVdI) (Bester and Maree 2022). To avoid post-amplification contamination, the colorimetric LAMP has been applied to detect viruses and viroids (Bhuvitarkorn et al 2019;Supakitthanakorn et al 2022b). Positive and negative colorimetric detection results are easily distinguishable by a visible color change, which reduces the complexity and duration of the detection procedure (Poole et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Journal of Plant Protection Research

Supakitthanakorn,

Vichittragoontavorn,

Kunasakdakul

et al. 2022

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Chrysanthemum stunt viroid (CSVd) is a serious pathogen infecting chrysanthemum worldwide. To improve and enhance the detection procedure, a colorimetric loop-mediated isothermal amplification (LAMP) technique was developed. Six LAMP primers were newly designed and tested to determine the optimal conditions using a recombinant plasmid of CSVd as a DNA template. The optimal conditions for colorimetric LAMP were incubation at 65°C for 45 min. Under these conditions, a ladder-like pattern of LAMP products was detected along with a change of color from pink to yellow in the positive reactions. Limits of the detection (LOD) of colorimetric LAMP were up to 1 fg • µl -1 of plasmid DNA concentration which was 10 4 times greater than that of polymerase chain reaction (PCR). The developed colorimetric LAMP was not cross reacted to other viruses and viroids. From detection of actual samples and chrysanthemum plantlets which were obtained from meristem tip culture, the colorimetric LAMP showed effective potential in detecting CSVd. Therefore, the colorimetric LAMP can be used as a main technique to detect CSVd and ensure CSVd-free chrysanthemum plantlet production due to its accuracy, rapidness and sensitivity.

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“…TMV is the first discovered virus that infects more than 150 different species 6 . TMV is transmitted by mechanical inoculation, grafting, and by many species of aphids in a no persistent manner 7 , and induces mosaic-like mottling and discoloration on the leaves 8 .…”

Section: Introductionmentioning

confidence: 99%

Recombinant anti-HIV MAP30, a ribosome inactivating protein: against plant virus and bacteriophage

Amirzadeh

Moghadam

Niazi‎

et al. 2023

Sci Rep

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The ribosome inactivating proteins (RIPs) efficiently decrease the microbial infections in plants. Momordicacharantia MAP30 is a type I RIP that has not been investigated against plant viruses or bacteriophages. To evaluate of these activities, the recombinant MAP30 (rMAP30) was produced in the hairy roots of Nicotianatabacum. Inoculation of 3 μg of transgenic total protein or 0.6 μg of rMAP30 against 0.1 μg of TMV reduced the leaf necrotic spots to 78.23% and 82.72%, respectively. The treatment of 0.1 μg of CMV with rMAP30 (0.6 μg) showed the reduction in the leaf necrotic spots to 85.8%. While the infection was increased after rMAP30 dilution. In the time interval assays, the leaves were first inoculated with 1 μg of rMAP30 or 0.1 μg of purified TMV or CMV agent for 6 h, then virus or protein was applied in order. This led the spot reduction to 35.22% and 67% for TMV, and 38.61% and 55.31% for CMV, respectively. In both the pre- and co-treatments of 1:10 or 1:20 diluted bacteriophage with 15 μg of transgenic total protein, the number and diameter of the plaques were reduced. The results showed that the highest inhibitory effect was observed in the pre-treatment assay of bacteriophage with transgenic total protein for 24 h. The decrease in the growth of bacteriophage caused more growth pattern of Escherichiacoli. The results confirm that rMAP30 shows antibacterial activity against Streptococcusaureus and E.coli, antifungal activity against Candidaalbicans, and antiviral activity against CMV and TMV. Moreover, rMAP30 exhibits anti-phage activity for the first time. According to our findings, rMAP30 might be a valuable preservative agent in foods and beverages in the food industry as well as an antiviral and antimicrobial mixture in agriculture.

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Tobacco Mosaic Virus Infection of Chrysanthemums in Thailand: Development of Colorimetric Reverse-Transcription Loop-Mediated Isothermal Amplification (RT–LAMP) Technique for Sensitive and Rapid Detection

Cited by 8 publications

References 38 publications

Current Achievements and Future Prospects in Virus Elimination Technology for Functional Chrysanthemum

Current Achievements and Future Prospects in Virus Elimination Technology for Functional Chrysanthemum

Journal of Plant Protection Research

Recombinant anti-HIV MAP30, a ribosome inactivating protein: against plant virus and bacteriophage

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