Tobacco chloroplast transformants expressing genes encoding dehydroascorbate reductase, glutathione reductase, and glutathione‐S‐transferase, exhibit altered anti‐oxidant metabolism and improved abiotic stress tolerance

Martret, Bénédicte Le; Poage, Miranda; Shiel, Karen; Nugent, Gregory D.; Dix, Philip J.

doi:10.1111/j.1467-7652.2011.00611.x

Cited by 183 publications

(110 citation statements)

References 54 publications

Supporting

Mentioning

102

Contrasting

Order By: Relevance

“…Furthermore, the importance of multi-component scavenging pathways, such as the ascorbate-glutathione cycle (Mittler 2002), together with the often limited effects of expressing individual components, suggest concerted expression of two or more genes might be more informative, something readily accomplished through chloroplast transformation (Bock 2001;Maliga 2004). This is illustrated by recent experiments in our laboratory (Le Martret et al 2011) in which we combine expression of dehydroascorbate reductase (DHAR) and glutathione reductase (GR) in tobacco chloroplasts. These two enzymes constitute the parts of the ascorbate-glutathione cycle responsible for regenerating ascorbate, and the co-expressing plants exhibit a pronounced increase in tolerance to methyl viologen-mediated oxidative stress, whereas expression of the individual enzymes has a neutral (DHAR) or negative (GR) outcome.…”

Section: Discussionmentioning

confidence: 99%

Modification of reactive oxygen species scavenging capacity of chloroplasts through plastid transformation

et al. 2011

Self Cite

View full text Add to dashboard Cite

Reactive oxygen species (ROS), including superoxide anions, hydrogen peroxide and hydroxyl radicals are generated through normal biochemical processes, but their production is increased by abiotic stresses. The prospects for enhancing ROS scavenging, and hence stress tolerance, by direct gene expression in a vulnerable cell compartment, the chloroplast, have been explored in tobacco. Several plastid transformants were generated which contained either a Nicotiana mitochondrial superoxide dismutase (MnSOD) or an Escherichia coli glutathione reductase (gor) gene. MnSOD lines had a three-fold increase in MnSOD activity, but interestingly a five to nine-fold increase in total chloroplast SOD activity. Gor transgenic lines had up to 6 times higher GR activity and up to 8 times total glutathione levels compared to wild type tobacco. Photosynthetic capacity of transplastomic plants, as measured by chlorophyll content and variable fluorescence of PSII was equivalent to non-transformed plants.The response of these transplastomic lines to several applied stresses was examined. In a number of cases improved stress tolerance was observed. Examples include enhanced methyl viologen (Paraquat)-induced oxidative stress tolerance in Mn-superoxidase dismutase overexpressing plants, improved heavy metal tolerance in glutathione reductase expressing lines, and improved tolerance to UV-B radiation in both sets of plants.

show abstract

Section: Discussionmentioning

confidence: 99%

Modification of reactive oxygen species scavenging capacity of chloroplasts through plastid transformation

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…GST plays an important role in the reduction of organic hydroperoxides formed during oxidative stress using the tripeptide glutathione (GSH) as a cosubstrate or coenzyme (Dixon et al, 2002). OE of a GST gene from Escherichia coli in transgenic tobacco enhanced salt and cold tolerance (Le Martret et al, 2011). In another study, transgenic Arabidopsis plants overexpressing a mustard (Brassica juncea) GST gene (BjGSTF2) exhibited tolerance to HgCl 2 and paraquat (Gong et al, 2005).…”

Section: Ospp18 Mediates Drought Stress Tolerance By Regulation Of Romentioning

confidence: 99%

A STRESS-RESPONSIVE NAC1-Regulated Protein Phosphatase Gene RiceProtein Phosphatase18Modulates Drought and Oxidative Stress Tolerance through Abscisic Acid-Independent Reactive Oxygen Species Scavenging in Rice

et al. 2014

View full text Add to dashboard Cite

Plants respond to abiotic stresses through a complexity of signaling pathways, and the dephosphorylation mediated by protein phosphatase (PP) is an important event in this process. We identified a rice (Oryza sativa) PP2C gene, OsPP18, as a STRESS-RESPONSIVE NAC1 (SNAC1)-regulated downstream gene. The ospp18 mutant was more sensitive than wild-type plants to drought stress at both the seedling and panicle development stages. Rice plants with OsPP18 suppressed through artificial microRNA were also hypersensitive to drought stress. Microarray analysis of the mutant revealed that genes encoding reactive oxygen species (ROS) scavenging enzymes were down-regulated in the ospp18 mutant, and the mutant exhibited reduced activities of ROS scavenging enzymes and increased sensitivity to oxidative stresses. Overexpression of OsPP18 in rice led to enhanced osmotic and oxidative stress tolerance. The expression of OsPP18 was induced by drought stress but not induced by abscisic acid (ABA). Although OsPP18 is a typical PP2C with enzymatic activity, it did not interact with SNF1-RELATED PROTEIN KINASE2 protein kinases, which function in ABA signaling. Meanwhile, the expression of ABA-responsive genes was not affected in the ospp18 mutant, and the ABA sensitivities of the ospp18 mutant and OsPP18-overexpressing plants were also not altered. Together, these findings suggest that OsPP18 is a unique PP2C gene that is regulated by SNAC1 and confers drought and oxidative stress tolerance by regulating ROS homeostasis through ABA-independent pathways.

show abstract

“…The expression pattern of these stress related genes were similar to the drought tolerant plants as reported earlier. [21][22][23][24] Among the up-accumulated proteins in response to drought stress in NtGp11 spot no. 4 and 13 had identified to HSP70 like proteins and spot no.…”

Section: Discussionmentioning

confidence: 99%

Proteomic profiling of γ-ECSoverexpressed transgenicNicotianain response to drought stress

Kumar

Datta²,

Sinha

et al. 2014

Plant Signaling & Behavior

View full text Add to dashboard Cite

The contribution of Glutathione (Gsh) in drought stress tolerance is an established fact. however, the proteins which are directly or indirectly related to the increased level of Gsh in response to drought stress are yet to be known. To explore this, here, transgenic tobacco plants (NtGp11) overexpressing gamma-glutamylcysteine synthetase (γ-ECS) was tested for tolerance against drought stress. NtGp11 conferred tolerance to drought stress by increased germination rate, water retention, water recovery, chlorophyll, and proline content compared with wild-type plants. semi-quantitative RT-PcR analysis revealed that the transcript levels of stress-responsive genes were higher in NtGp11 compared with wild-type in response to drought stress. Two-dimensional gel electrophoresis (2-De) coupled with MaLDI TOF-TOF Ms/Ms analysis has been used to identify 43 differentially expressed proteins in response to drought in wild-type and NtGp11 plants. The results demonstrated the up-accumulation of 58.1% of proteins among which 36%, 24%, and 20% of them were related to stress and defense, carbon metabolism and energy metabolism categories, respectively. Taken together, our results demonstrated that Gsh plays an important role in combating drought stress in plants by inducing stress related genes and proteins like hsP70, chalcone synthase, glutathione peroxidase, thioredoxin peroxidase, acc oxidase, and heme oxygenase I.

show abstract

Tobacco chloroplast transformants expressing genes encoding dehydroascorbate reductase, glutathione reductase, and glutathione‐S‐transferase, exhibit altered anti‐oxidant metabolism and improved abiotic stress tolerance

Cited by 183 publications

References 54 publications

Modification of reactive oxygen species scavenging capacity of chloroplasts through plastid transformation

Modification of reactive oxygen species scavenging capacity of chloroplasts through plastid transformation

A STRESS-RESPONSIVE NAC1-Regulated Protein Phosphatase Gene RiceProtein Phosphatase18Modulates Drought and Oxidative Stress Tolerance through Abscisic Acid-Independent Reactive Oxygen Species Scavenging in Rice

Proteomic profiling of γ-ECSoverexpressed transgenicNicotianain response to drought stress

Contact Info

Product

Resources

About