Tobacco

Sheen, S. J.

doi:10.1016/b978-0-444-42227-9.50014-7

Cited by 4 publications

(2 citation statements)

References 60 publications

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“…A number of studies have demonstrated that the variation in isozyme patterns is related to the growth environment of tobacco cell and tissue cultures (Sheen, 1983). In the present study, the mMDH isozyme observed in seeds but not in seedlings became detectable in callus in the presence of phytohormone combinations.…”

Section: Discussionmentioning

confidence: 82%

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Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes in tissues and callus cultures ofCereus peruvianus (cactaceae)

1993

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Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes were investigated in seeds and in seedlings and calli cultures of C. peruvianus to determine if the changes in MDH isozyme banding patterns could be used as biochemical markers to identify the origin of regenerated plants from callus tissues. Four cytoplasmic MDH isozymes (sMDH), five mitochondrial MDH isozymes (mMDH), and one glyoxysomal MDH isozyme (gMDH) were detected and showed tissue- and stage-specific expression. A relationship of mMDH and gMDH isozyme patterns with callus tissues subcultured in three hormonal combinations and with the plants regenerated from these callus tissues was demonstrated. Furthermore, temperature and mechanical stress were found to be closely related to mMDH-1 activity in callus culture. Therefore, the different patterns of MDH isozymes in the various tissues of C. peruvianus can be used as biochemical markers for the study of gene expression during development and as powerful tools in monitoring studies on callus cultures.

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Section: Discussionmentioning

confidence: 82%

“…According to Sheen (1983), inhibition or slow callus growth caused a significant increase in anodic peroxidase, with slow electrophoretic mobilities. Likewise, the growth of C. peruvianus callus subcultured at 22°C was fully inhibited after 20 days in subculture.…”

Section: Discussionmentioning

confidence: 99%

Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes in tissues and callus cultures ofCereus peruvianus (cactaceae)

1993

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Alcohol dehydrogenase (EC 1.1.1.1) isozymes as markers at 2,4-dichlorophenoxyacetic acid � kinetin combinations in callus cultures ofCereus peruvianus (Cactaceae)

1994

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Alcohol dehydrogenase (ADH; EC 1.1.1.1) isozymes were investigated in tissue of Cereus peruvianus cultured in different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin. Five ADH isozymes were detected in starch gel and showed different patterns in seeds, seedlings, calli cultured at 32 and 22 degrees C, and plants regenerated from calli cultured in three 2,4-D and kinetin combinations. Four phenotypes formed by different combinations of ADH-2, ADH-3, ADH-4, and ADH-5 were detected in calli cultured at 32 degrees C and in plants regenerated from calli. ADH-1 isozyme was detected only in calli subcultured for 1 or 2 weeks at 22 degrees C and was indicated as a marker of stress conditions that affect the growth of C. peruvianus callus tissues in culture. ADH phenotypes with either a higher or a lower number of isozymes were detected in different proportions in the callus tissues cultured in media containing different 2,4-D and kinetin ratios. ADH isozyme patterns were found to be sensitive markers at the highest kinetin concentration or at high kinetin/2,4-D ratios. The results indicate a high correlation between the ADH isozyme patterns and the capacity for regeneration. Thus, ADH isozymes are indicated as good biochemical markers and as a powerful tool for monitoring studies of C. peruvianus callus cultures.

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Cytogenetic and isozymic characterization of anther-panicle culture derived tall fescue aneuploids

Eizenga

1987

Euphytica

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Tobacco

Cited by 4 publications

References 60 publications

Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes in tissues and callus cultures ofCereus peruvianus (cactaceae)

Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes in tissues and callus cultures ofCereus peruvianus (cactaceae)

Alcohol dehydrogenase (EC 1.1.1.1) isozymes as markers at 2,4-dichlorophenoxyacetic acid � kinetin combinations in callus cultures ofCereus peruvianus (Cactaceae)

Cytogenetic and isozymic characterization of anther-panicle culture derived tall fescue aneuploids

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