To the intranucleolar translocation of AgNORs in leukemic early granulocytic and plasmacytic precursors

Abstract

Early leukemic granulocytic and plasmacytic precursors were studied in vitro and in vivo to provide an information on the intranucleolar distribution of AgNORs (silver stained nucleolus organizer regions). In most of these cells AgNORs appeared as clusters of silver stained particles distributed in the whole nucleolar body. On the other hand, in some leukemic early granulocytic precursors, i.e., in myeloblasts and promyelocytes enlarged AgNORs were translocated in the nucleolar peripheral part. In addition, the number of translocated AgNORs at the nucleolar periphery was significantly smaller. Such translocation of a reduced number of AgNORs was easily produced by experimental aging, i.e., starving of cultured leukemic early granulocytic precursors (HL-60 and K562 cells) in vitro and seems to be reversible. Similar translocation of a reduced number of AgNORs was also produced by aging of leukemic plasmacytic precursors. Thus, the translocation of the reduced number of AgNORs to the nucleolar periphery in some blastic leukemic hematopoietic cells might be an useful marker of their aging at the single cell level. However, more studies in this direction are required in the future.

Age-Dependent Change in the Morphology of Nucleoli and Methylation of Genes of the Nucleolar Organizer Region in Japanese Quail (<I>Coturnix japonica</I>) Model (Temminck and Schlegel, 1849) (Galliformes: Aves)

“…The morphometric parameters of nucleoli are number, size and distance from the nuclear membrane (NAFE & SCHLOTE 2004;SMETANA et al 2005SMETANA et al , 2006RAŠKA et al 2006). Taking into account previous observations by MONTGOMERY (1895), biologists researching tumours quickly created a link between stained AgNOR and cell proliferation (DE-RENZINI 1990;RAŠKA et al 2006).…”

Structure of the nucleoli in domestic cattle spermatocytes

“…Nucleolar morphology has been one of the key criteria of neoplasm classification. The morphometric parameters of nucleoli are the number, size and distance from the nuclear membrane [3,[28][29][30]. Taking advantage of observations made by Montgomery (1895), biologists dealing with neoplasms quickly tracked down the connection between AgNOR stains and cell proliferation [2,3,31].…”

The histochemistry and cell biology vade mecum: a review of 2005–2006

“…Collectively, this demonstrates a signiWcant decrease in NDH II gene expression and in NDH II mRNA translation in correlation with termination of oocyte growth, and that NDH II expression parallels the activity of ribosomal RNA biosynthesis in growing bovine oocytes. Smetana et al (2006a) studied early leukemic granulocytic and plasmacytic precursors in vitro and in vivo to gain information on the intranucleolar distribution of AgNORs (silver stained nucleolus organizer regions). In most cells, AgNORs appeared as clusters in the whole nucleolar body.…”