Background: Prevalence of hamstring muscle tightness among undergraduate students shows 40.17%. Hamstring muscle tightness is "inability to extend the knee completely when the hip is flexed accompanied by discomfort or pain along the posterior thigh and/or knee is usually attributed to. Dynamic soft tissue mobilization developed with the aim of increase muscle length. It utilizes combine technique classic massage followed by dynamic component with the limb is moved through its range. Retro -walking is a dynamic activity which can improve be used to treat the flexibility as well as dynamic balance. Aim: To find out the effect of dynamic soft tissue mobilization with retro -walking on hamstring flexibility and dynamic balance in young collegiate students. Methodology: 25 subjects were selected Based on the selection criterion, the whole procedure of the study was explained to all the subjects. Prior and after to treatment both the outcome measures, Active Knee Extension Test and Y -Balance Test were measured. Intervention in the form of Dynamic Soft Tissue Mobilization Technique with Retro -walking were given alternate 3 sessions per week for 4 weeks. Results and Discussion: The data were analysed by Paired t test. P value for both the outcome measures were <0.05 which shows significant improvement by improving flexibility and dynamic balance. Conclusion: Intervention of the study was effective, very simple and easy to apply in Hamstring tightness subjects. So it can be implemented clinically as well.