To involvement the conformation of the adenine nucleotide translocase in opening the Tl+-induced permeability transition pore in Ca2+-loaded rat liver mitochondria

Коротков, С. М.; Konovalova, Svetlana; Brailovskaya, I. V.; Saris, Nils‐Erik L.

doi:10.1016/j.tiv.2016.01.015

Cited by 21 publications

(24 citation statements)

References 83 publications

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“…On the other hand, the even greater decline in state 3U DNP and the RCR DNP in the presen ce of NEM (Fig. 5, c and d) is most likely associa ted with the inhibition of succinate dehydrogenase that we observed earlier in similar experiments with RLM [21]. Above results of these experiments with Ca 2+ loaded RHM (Figs.…”

Section: Resultssupporting

confidence: 62%

“…In our experiments with Ca 2+ loaded RLM, the Tl + induced MPTP opening in medium containing TlNO 3 and nitrates (KNO 3 , NaNO 3 , NH 4 NO 3 ) was facilitated by inor ganic phosphate [43] but impeded in the presence of both the MPTP inhibitors (ADP, CsA, Mg 2+ ) and the PiC inhibitor mersalyl [15,45]. This allowed us to reach a conclusion regarding the participation of the ANT and the PiC in the Tl + induced MPTP opening in the inner membrane [21,45]. We earlier found that a closure of mitochondrial potassium chan nels (mitoK ATP and mitoK Ca ) increased the MPTP opening [46].…”

Section: Resultsmentioning

confidence: 82%

“…legend. The use of millimolar thallium(I) concentrations for our study was due to the specifics of isolated mitochondria research that was our discussed earlier [20,21]. Statistical analysis.…”

Section: Methodsmentioning

confidence: 99%

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Influence of Tl(+) on the Ca(2+) and Na(+) movement across rat neonatal cardiomyocytes and rat heart mitochondria membranes

et al. 2020

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thallium is known to produce one of the most complex and serious patterns of toxicity, involving a wide range of human organs and tissues. the toxic impact on biologic organisms is linked especially to the ability of tl + to disturb calcium homeostasis and to permeate easily the inner mitochondrial membrane (IMM). the aim of this work was to study the effects of Tl + on intracellular Ca 2+ dynamics in rat neonatal cardiomyocytes as well as on sodium penetrability of the IMM and tl + -induced mitochondrial permeability transition pore (MPtP) opening in isolated Ca 2+ -loaded rat heart mitochondria (RHM). The use of the fluorescent calcium indicator Fura 2 aM showed that tl + induced calcium influx across the plasmatic membrane, resulting in calcium ([Ca 2+ ] i ) increase in the cytoplasm. this increase was even more pronounced in experiments with accelerating of tl + -transmembrane fluxes by nonactin. It was nevertheless abolished by the removal of extracellular Ca 2+ ions, but was not inhibited by a calcium-channel blocker (nifedipine). tl + did not release calcium from the intracellular stores. tl + potentiated sodium permeability of the IMM because swelling of nonenergized rhM in medium containing tlNo 3 and NaNo 3 was enhanced at high tl + concentration. the calcium load of rhM induced MPtP opening which was accompanied by the increase of the swelling as well as the decrease of the inner membrane potential and of state 4 0 (basal) and state 3U DNP (2,4-dinitrophenol-uncoupled) respiration. These effects of Tl + were suppressed by MPtP inhibitors (cyclosporine a, aDP and n-ethylmaleimide). the data obtained showed that tl + -stimulated influx of extracellular calcium into cardiomyocytes could cause calcium and sodium rhM overload, which lead to the MPtP opening, thus determining the sensitivity of heart muscle to thallium intoxication. K e y w o r d s: tl + , Ca 2+ , Na + , cardiomyocytes, rat heart mitochondria, mitochondrial permeability transition pore (MPtP).

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Section: Resultssupporting

confidence: 62%

Section: Resultsmentioning

confidence: 82%

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Influence of Tl(+) on the Ca(2+) and Na(+) movement across rat neonatal cardiomyocytes and rat heart mitochondria membranes

et al. 2020

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“…It was concluded that tBHP oxidizes the vicinal SH-groups of mitochondrial membrane to disulphides, which results in higher gating potential (or higher pore opening probability) (Petronilli et al1994). Stabilization of the ANT in "c" conformation by oxidative stress inductors (tBHP and diamide) due to reaction with thiol groups was found to increase MPT pore opening in the inner mitochondrial membrane (Castilho et al 1996;Halestrap and Brenner, 2003;Halestrap 2010;Korotkov et al 2016). Recently, it has been shown that Ca 2+ -induced swelling enhanced by tBHP was inhibited by ADP and hydrophobic thiol reagent, N-ethylmaleimide, and CsA additionally increased these effects of ADP and N-ethylmaleimide (Korotkov et al, 2016).…”

Section: Figmentioning

confidence: 99%

“…Stabilization of the ANT in "c" conformation by oxidative stress inductors (tBHP and diamide) due to reaction with thiol groups was found to increase MPT pore opening in the inner mitochondrial membrane (Castilho et al 1996;Halestrap and Brenner, 2003;Halestrap 2010;Korotkov et al 2016). Recently, it has been shown that Ca 2+ -induced swelling enhanced by tBHP was inhibited by ADP and hydrophobic thiol reagent, N-ethylmaleimide, and CsA additionally increased these effects of ADP and N-ethylmaleimide (Korotkov et al, 2016). Ca 2+ release from the damaged mitochondria plays a role in this process because EDTA (0.5 mM) inhibited membrane depolarization induced by tBHP.…”

Section: Figmentioning

confidence: 99%

Calcium-Induced Mitochondrial Permeability Transitions: Parameters of Ca2+ Ion Interactions with Mitochondria and Effects of Oxidative Agents

et al. 2017

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We evaluated the parameters of Ca-induced mitochondrial permeability transition (MPT) pore formations, Ca binding constants, stoichiometry, energy of activation, and the effect of oxidative agents, tert-butyl hydroperoxide (tBHP), and hypochlorous acid (HOCl), on Ca -mediated process in rat liver mitochondria. From the Hill plot of the dependence of MPT rate on Ca concentration, we determined the order of interaction of Ca ions with the mitochondrial sites, n = 3, and the apparent K = 60 ± 12 µM. We also found the apparent Michaelis-Menten constant, K, for Ca interactions with mitochondria to be equal to 75 ± 20 µM, whereas that in the presence of 300 µM tBHP was 120 ± 20 µM. Using the Arrhenius plots of the temperature dependences of apparent mitochondrial swelling rate at various Ca concentrations, we calculated the activation energy of the MPT process. ΔE was 130 ± 20 kJ/mol at temperatures below the break point of the Arrhenius plot (30-34 °C) and 50 ± 9 kJ/mol at higher temperatures. Ca ions induced rapid mitochondrial NADH depletion and membrane depolarization. Prevention of the pore formation by cyclosporin A inhibited Ca-dependent mitochondrial depolarization and Mg ions attenuated the potential dissipation. tBHP (10-150 µM) dose-dependently enhanced the rate of MPT opening, whereas the effect of HOCl on MPT depended on the ratio of HOCl/Ca. The apparent K of tBHP interaction with mitochondria in the swelling reaction was found to be K = 11 ± 3 µM. The present study provides evidence that three calcium ions interact with mitochondrial site with high affinity during MPT. Ca-induced MPT pore formations due to mitochondrial membrane protein denaturation resulted in membrane potential dissipation. Oxidants with different mechanisms, tBHP and HOCl, reduced mitochondrial membrane potential and oxidized mitochondrial NADH in EDTA-free medium and had an effect on Ca-induced MPT onset.

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Ordered Conformation‐Regulated Vesicular Membrane Permeability

Zheng

Wang

et al. 2021

Angewandte Chemie

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In nature, the folding and conformation of proteins can control the cell or organelle membrane permeability and regulate the life activities. Here we report the first example of synthetic polypeptide vesicles that regulate their permeability via ordered transition of secondary conformations, in a manner similar to biological systems. The polymersomes undergo a β‐sheet to α‐helix transition in response to reactive oxygen species (ROS), leading to wall thinning without loss of vesicular integrity. The change of membrane structure increases the vesicular permeability and enables specific transport of payloads with different molecular weights. As a proof‐of‐concept, the polymersomes encapsulating enzymes could serve as nanoreactors and carries for glucose‐stimulated insulin secretion in vivo inspired by human glucokinase, resulting in safe and effective treatment of type 1 diabetes mellitus in mouse models. This study will help understand the biology of biomembranes and facilitate the engineering of nanoplatforms for biomimicry, biosensing, and controlled delivery applications.

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To involvement the conformation of the adenine nucleotide translocase in opening the Tl+-induced permeability transition pore in Ca2+-loaded rat liver mitochondria

Cited by 21 publications

References 83 publications

Influence of Tl(+) on the Ca(2+) and Na(+) movement across rat neonatal cardiomyocytes and rat heart mitochondria membranes

Influence of Tl(+) on the Ca(2+) and Na(+) movement across rat neonatal cardiomyocytes and rat heart mitochondria membranes

Calcium-Induced Mitochondrial Permeability Transitions: Parameters of Ca2+ Ion Interactions with Mitochondria and Effects of Oxidative Agents

Ordered Conformation‐Regulated Vesicular Membrane Permeability

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