To Identify Drug-Drug Interaction in Cardiac Patients in Tertiary Care Hospitals

The analytical technique of choice for separating, identifying, and quantifying complex mixtures is high-performance liquid chromatography (HPLC). Reverse-phase liquid chromatography (RP-HPLC) is the preferred separation mode for high-performance liquid chromatography (HPLC) due to its adaptability and higher selectivity for hydrophobic compounds. This review article discusses the fundamentals of reversed-phase high-performance liquid chromatography (RP-HPLC). This covers the separation principle, various stationary and mobile phase types, and separation-affecting variables. This article highlights the need of developing and testing such methods in addition to outlining the advantages of using RP-HPLC in industries like pharmaceutical, food, and environmental analysis. As examples of more recent advancements in RP-HPLC, new stationary and mobile phases, RP-HPLC downsizing, and hyphenated methods are also discussed. This review article provides a comprehensive tool for designing, refining, and validating RP-HPLC processes.

Section: Fundamentals Of Rp-hplcmentioning

confidence: 99%

A Comprehensive Review on Analytical Method Development using RP-HPLC and Recent Advances in Pharmaceutical Applications

Kumar¹,

Kumar²,

Kumar³

et al. 2023

“…Recent developments in the technology used to sequence DNA have made it possible to sensitively identify epigenetic alterations in circulating cell-free DNA [44,45]. This could pave the way for diagnostic methods that are less invasive.…”

Section: [Citation Needed]mentioning

confidence: 99%

Herbal used as Epigenetic for Cancer Treatment

Navadiya¹,

Goti²,

Raloliya³

et al. 2023

Patterns of chromatin accessibility, which are in turn altered by epigenetic mechanisms, are responsible for determining the interactions that take place between transcription machinery, genes, and the cis-regulatory elements of those genes. Mutations that interfere with epigenetic processes frequently have the unfortunate side effect of causing cancer. Since these mutations can be undone, numerous anticancer treatments that target epigenetic pathways are now being developed and evaluated. (Cancer) is becoming more and more common everywhere. This decline in clinical outcomes is a result of a number of factors, including late diagnosis, a lack of efficient treatments for particular cancer subtypes, and drug resistance. Treatment resistance and stemness traits have been recognised as markers of this disease, and it has been demonstrated that epigenetic changes play a role in the process of cancer growth. Understanding these alterations and how they impact cancer carcinogenesis treatment is challenging but crucial. However, it may be able to provide the special knowledge needed to use these alterations as potential diagnostic, prognostic, therapeutic agents, and predictors of treatment efficacy. This underlines the importance of continued research to advance our knowledge of cancer carcinogenesis and epigenetics and help us overcome these challenges. This review aims to provide an overview of the state of the art in epigenetics research for cancer detection and treatment and to stimulate discussion on this subject.

“…Both the retina and the ONH show upregulation of genes involved in inflammatory pathways (77,78). TLR signaling pathways are the first to be activated; for instance, HSPs boost MHC class II expression and cytokine production (79).…”

Section: Microglia and Astrogliamentioning

confidence: 99%

Recent Approaches of Ocular Disease and Its Herbal Product Treatment: An Updates

Butola¹,

Bisht²,

Kumar³

2023

One of the most common causes of permanent vision loss is glaucoma. Damage to the optic nerve and retinal ganglion cells, the root cause of visual field impairment, is typically the result of elevated intraocular pressure. Some patients' illness worsens even when treated with eye medications that reduce intraocular pressure. The pathophysiology of glaucoma is not only thought to involve mechanical and vascular dysfunctions of the eye, but also oxidative stress, neuroinflammation, and excitotoxicity. Therefore, natural compounds with antioxidant and anti-inflammatory qualities may offer a different strategy for treating glaucoma. This review summarizes the most up-to-date preclinical and clinical research on natural compounds that have been proven to contain neuroprotective characteristics for retinal ganglion cells and, consequently, may be useful in the treatment of glaucoma. Baicalein, forskolin, marijuana, ginsenoside, resveratrol, and hesperidin are all effective at lowering intraocular pressure. On the other hand, antioxidant, anti-inflammatory, and anti-apoptosis mechanisms are among those through which Ginkgo biloba, More research is needed to determine whether natural items can be used as a safe and effective treatment for glaucoma.