Aims:
The aim of this study was to evaluate the biocompatibility of resinous root canal sealers: Sealer 26, AH plus, and SK Seal Root Canal Sealer in the subcutaneous tissue of rats.
Subjects and Methods:
Twenty-four Wistar rats received polyethylene tubes containing the sealers and empty tubes as control (
n
= 6). After 7, 15, 30, and 60 days, animals were killed and polyethylene tubes were removed with the surrounding tissues. The specimens were embedded in paraffin, processed for hematoxylin-eosin and immunohistochemistry assessed for fibronectin (FN) and tenascin (TN).
Statistical Analysis Used:
Data were tabulated and analyzed via Kruskal–Wallis and Dunn's test (
P
< 0.05).
Results:
All groups induced a moderate inflammatory reaction after 7 and 15 days (
P
> 0.05); after 30 days, a mild inflammatory infiltrate was observed in control groups, and moderate in sealers groups (
P
> 0.05); all groups showed mild inflammatory infiltrate at 60 days (
P
> 0.05). Overall, the fibrous capsule was considered thick only on the 7
th
day and became thin over time. All groups had expression for FN and TN in all analyzed periods, with high immunolabeling in sealers groups when comparing with the control group (
P
< 0.05).
Conclusion:
All sealers demonstrated biocompatibility and induced FN and TN expression.