To die or not to die – How mitochondrial processes affect lifespan of Podospora anserina

Hamann, Andréa; Osiewacz, Heinz D.

doi:10.1016/j.bbabio.2022.148568

Cited by 3 publications

(2 citation statements)

References 102 publications

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“…Standard respiration in animals proceeds via complex I (NADH:ubiquinone oxidoreductase, inhibited by rotenone) or complex II (succinate dehydrogenase), complex III (ubiquinol:cytochrome c oxidoreductase), and complex IV (cytochrome c oxidase (COX), inhibited by potassium cyanide, KCN). In fila-mentous fungi several alternative respiratory chain components including the above-mentioned aNADH-DHs and an alternative terminal oxidase (AOX) are active under certain conditions [ 22 , 67 ]. In particular, the AOX (inhibited by SHAM) is well characterized.…”

Section: Resultsmentioning

confidence: 99%

“…Loss of the mitochondrial PRO34 leads to respiratory defects due to lack of complex I and incorrect assembly of mitochondrial supercomplexes. Feeding electrons into the respiratory chain thus might proceed via complex II or alternative complexes (i.e., alternative NADH dehydrogenases) which are commonly found in fungi [ 22 ]. Although pro34 respires via complexes III and IV of the canonical respiratory chain pathway, surprisingly, concomitant induction of the alternative oxidase (AOX) is observed.…”

Section: Introductionmentioning

confidence: 99%

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Sordaria macrospora Sterile Mutant pro34 Is Impaired in Respiratory Complex I Assembly

Hamann

Osiewacz

Teichert

2022

JoF

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The formation of fruiting bodies is a highly regulated process that requires the coordinated formation of different cell types. By analyzing developmental mutants, many developmental factors have already been identified. Yet, a complete understanding of fruiting body formation is still lacking. In this study, we analyzed developmental mutant pro34 of the filamentous ascomycete Sordaria macrospora. Genome sequencing revealed a deletion in the pro34 gene encoding a putative mitochondrial complex I assembly factor homologous to Neurospora crassa CIA84. We show that PRO34 is required for fast vegetative growth, fruiting body and ascospore formation. The pro34 transcript undergoes adenosine to inosine editing, a process correlated with sexual development in fruiting body-forming ascomycetes. Fluorescence microscopy and western blot analysis showed that PRO34 is a mitochondrial protein, and blue-native PAGE revealed that the pro34 mutant lacks mitochondrial complex I. Inhibitor experiments revealed that pro34 respires via complexes III and IV, but also shows induction of alternative oxidase, a shunt pathway to bypass complexes III and IV. We discuss the hypothesis that alternative oxidase is induced to prevent retrograde electron transport to complex I intermediates, thereby protecting from oxidative stress.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sordaria macrospora Sterile Mutant pro34 Is Impaired in Respiratory Complex I Assembly

Hamann

Osiewacz

Teichert

2022

JoF

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The bZIP-type transcription factors NapA and RsmA modulate the volumetric ratio and the relative superoxide ratio of mitochondria in Aspergillus nidulans

Bákány,

Antal,

Szentesi

et al. 2023

BIOLOGIA FUTURA

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Basic leucine zipper (bZIP) transcription factors are crucial components of differentiation, cellular homeostasis and the environmental stress defense of eukaryotes. In this work, we further studied the consequence of gene deletion and overexpression of two bZIP transcription factors, NapA and RsmA, on superoxide production, mitochondrial morphology and hyphal diameter of Aspergillus nidulans. We have found that reactive oxygen species production was influenced by both gene deletion and overexpression of napA under tert-butylhydroperoxide (tBOOH) elicited oxidative stress. Furthermore, gene expression of napA negatively correlated with mitochondrial volumetric ratio as well as sterigmatocystin production of A. nidulans. High rsmA expression was accompanied with elevated relative superoxide ratio in the second hyphal compartment. A negative correlation between the expression of rsmA and catalase enzyme activity or mitochondrial volumetric ratio was also confirmed by statistical analysis. Hyphal diameter was independent on either rsmA and napA expression as well as 0.2 mM tBOOH treatment.

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Podospora anserina

Ament‐Velásquez

Vogan

2022

Trends in Microbiology

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To die or not to die – How mitochondrial processes affect lifespan of Podospora anserina

Cited by 3 publications

References 102 publications

Sordaria macrospora Sterile Mutant pro34 Is Impaired in Respiratory Complex I Assembly

Sordaria macrospora Sterile Mutant pro34 Is Impaired in Respiratory Complex I Assembly

The bZIP-type transcription factors NapA and RsmA modulate the volumetric ratio and the relative superoxide ratio of mitochondria in Aspergillus nidulans

Podospora anserina

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