TNP1 Functional SNPs in bta-miR-532 and bta-miR-204 Target Sites Are Associated with Semen Quality Traits in Chinese Holstein Bulls1

Zhang, Shuai; Zhang, Yan; Yang, Chunhong; Zhang, Wei; Ju, Zhihua; Wang, Xiuge; Jiang, Qiang; Sun, Yan; Huang, Jingming; Zhong, Jun; Wang, Changfa

doi:10.1095/biolreprod.114.126672

Cited by 19 publications

(18 citation statements)

References 48 publications

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“… 25 found that SIRT1 is the direct target gene of miR-204-5p 38 , and SIRT1 has been demonstrated to promote the expression of adipose triglyceride lipase gene and glycerol release 39 . Moreover, bta-miR-204 was determined to be present in the reproductive organs and was found to be significantly associated with semen quality traits in bovines 40 . These results indicated that bta-miR-204 may be involved in lipid metabolism in cattle and may be regulated by testosterone.…”

Section: Discussionmentioning

confidence: 99%

MiR-27a-5p Increases Steer Fat Deposition Partly by Targeting Calcium-sensing Receptor (CASR)

Tang

Wang

Zan

2018

Sci Rep

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Castration increases fat deposition, improving beef quality in cattle. Here, the steer group exhibited a significantly higher intramuscular fat (IMF) content than the bull group. To determine the potential roles of microRNAs (miRNAs) in castration-induced fat deposition, differential expression patterns of miRNA in liver tissue were investigated in bulls and steers. A total of 7,827,294 clean reads were obtained from the bull liver library, and 8,312,483 were obtained from the steer liver library; 452 conserved bovine miRNAs and 20 novel miRNAs were identified. The results showed that the expression profiles of miRNA in liver tissue were changed by castration, and 12 miRNAs that were differentially expressed between bulls and steers were identified. Their target genes were majorly involved in the metabolic, PI3K-Akt, and MAPK signaling pathways. Furthermore, six differentially expressed miRNAs were validated by quantitative real-time PCR, and luciferase reporter assays verified that calcium-sensing receptor (CASR) was the direct target of miR-27a-5p. Meantime, we found that the expression level of CASR was significantly higher in steers than in bulls, and revealed that CASR gene silencing in bovine hepatocytes significantly inhibited triacylglycerol (TAG) accumulation and reduced secretion of very low density lipoprotein (VLDL). These results obtained in the liver indicate that miR-27a-5p may increase fat deposition partly by targeting CASR in steers.

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Section: Discussionmentioning

confidence: 99%

MiR-27a-5p Increases Steer Fat Deposition Partly by Targeting Calcium-sensing Receptor (CASR)

Tang

Wang

Zan

2018

Sci Rep

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“…The relationship between the genotype or haplotype combinations of the INCENP gene and semen quality traits was analyzed using the least squares method as applied in the general linear models (GLM) procedure in SAS 9.0 (SAS Institute Inc., Cary, NC, USA). The GLM is given as follows [ 15 ]: where Y ijkl is the observed value of each semen quality trait, μ is the overall mean, G i is the fixed effect of genotype or haplotype combinations, P j is the fixed effect of age [j = 2–10; classified as (1) 2 years to 3 years; (2) 4 years to 5 years; (3)≥ 6 years], S k is the fixed effect of the origin of the bull, M l is the effect of the farm, and e ijkl is the random residual error. Values at P < 0 .…”

Section: Methodsmentioning

confidence: 99%

“…MicroRNAs are involved in multiple biological phenomena, such as spermatogenesis and early embryogenesis and tumorigenesis [ 12 , 13 , 14 ]. The SNPs in the binding site of miRNA, namely 3'UTR of the targeted mRNA, have been considered the principal element of microRNA adhesion and are associated with a phenotype, such as semen quality trait [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Functional SNPs of INCENP Affect Semen Quality by Alternative Splicing Mode and Binding Affinity with the Target Bta-miR-378 in Chinese Holstein Bulls

et al. 2016

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Inner centromere protein (INCENP) plays an important role in mitosis and meiosis as the main member of chromosomal passenger protein complex (CPC). To investigate the functional markers of the INCENP gene associated with semen quality, the single nucleotide polymorphisms (SNPs) g.19970 A>G and g.34078 T>G were identified and analyzed. The new splice variant INCENP-TV is characterized by the deletion of exon 12. The g.19970 A>G in the exonic splicing enhancer (ESE) motif region results in an aberrant splice variant by constructing two minigene expression vectors using the pSPL3 exon capturing vector and transfecting vectors into MLTC-1 cells. INCENP-TV was more highly expressed than INCENP-reference in adult bull testes. The g.34078 T>G located in the binding region of bta-miR-378 could affect the expression of INCENP, which was verified by luciferase assay. To analyze comprehensively the correlation of SNPs with sperm quality, haplotype combinations constructed by g.19970 A>G and g.34078 T>G, as well as g.-692 C>T and g.-556 G>T reported in our previous studies, were analyzed. The bulls with H1H12 and H2H2 exhibited a higher ejaculate volume than those with H2H10 and H9H12, respectively (P < 0.05). Bulls with H11H11 and H2H10 exhibited higher initial sperm motility than those with H2H2 (P < 0.05). The expression levels of INCENP in bulls with H1H12 and H11H11 were significantly higher than those in bulls with H9H12 (P < 0.05), as determined by qRT-PCR. Findings suggest that g.19970 A>G and g.34078 T>G in INCENP both of which appear to change the molecular and biological characteristics of the mRNA transcribed from the locus may serve as a biomarkers of male bovine fertility by affecting alternative splicing mode and binding affinity with the target bta-miR-378.

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“…Zhang et al . 19 provided the first indication of g.442A > G-mediated and g.528G > A-mediated translational suppression in which SNPs alter the binding of bta-miR-204 and bta-miR-532 to the 3′-UTR of TNP1; the mediated translational suppression could be involved in the regulation of TNP1 expression and may influence the morphological characteristics of Chinese Holstein bull sperm. Liu et al .…”

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confidence: 98%

Association of polymorphisms at the microRNA binding site of the caprine KITLG 3′-UTR with litter size

Song

et al. 2016

Sci Rep

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This study identified three novel single nucleotide polymorphisms (SNPs) (c.1389C > T, c.1457A > C and c.1520G > A) in the caprine KITLG 3′-UTR through DNA sequencing. The three SNP loci were closely linked in Guanzhong dairy (GD) goats. Two alleles of the c.1457A > C SNP introduced two miRNA sites (chi-miR-204-5p and chi-miR-211). Individuals with combined genotype TT-CC-AA had a higher litter size compared with those with combined genotypes CC-AA-GG, TC-CC-GA and CC-AC-GG (P < 0.05). Luciferase assays showed that chi-miR-204-5p and chi-miR-211 suppressed luciferase expression in the presence of allele 1457A compared with negative control (NC) and allele 1457C (P < 0.05). Western blot revealed that KITLG significantly decreased in the granulosa cells (GCs) of genotype AA compared with that in the GCs of genotype CC and NC (P < 0.05). The KITLG mRNA levels of the CC-AA-GG carriers significantly decreased compared with those of the TT-CC-AA, TC-CC-GA and CC-AC-GG carriers. In addition, cell proliferation was reduced in haplotype C-A-G GCs compared with that in haplotype T-C-A GCs. These results suggest that SNPs c.1389C > T, c.1457A > C and c.1520G > A account for differences in the litter size of GD goats because chi-miR-204-5p and chi-miR-211 could change the expression levels of the KITLG gene and reduce GC proliferation.

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TNP1 Functional SNPs in bta-miR-532 and bta-miR-204 Target Sites Are Associated with Semen Quality Traits in Chinese Holstein Bulls1

Cited by 19 publications

References 48 publications

MiR-27a-5p Increases Steer Fat Deposition Partly by Targeting Calcium-sensing Receptor (CASR)

MiR-27a-5p Increases Steer Fat Deposition Partly by Targeting Calcium-sensing Receptor (CASR)

Functional SNPs of INCENP Affect Semen Quality by Alternative Splicing Mode and Binding Affinity with the Target Bta-miR-378 in Chinese Holstein Bulls

Association of polymorphisms at the microRNA binding site of the caprine KITLG 3′-UTR with litter size

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