TNF-α Upregulates Expression of BMP-2 and BMP-3 Genes in the Rat Dental Follicle—Implications for Tooth Eruption

Yao, Shaomian; Prpić, Veronica; Pan, Feng; Wise, Gary E.

doi:10.3109/03008200903019703

Cited by 29 publications

(21 citation statements)

References 32 publications

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“…Studies in our lab have determined that the bone formation at the base of tooth crypt likely serves as an eruption force to push the tooth out of its bony crypt (Wise et al, 2007; Wise et al, 2011). To determine what molecules the DF uses to regulate the bone formation needed for tooth eruption, we examined the expression of bone morphogenetic proteins (BMPs) in the DF, and reported the expression of BMP2, BMP3 and BMP6 in the DF (Wise and Yao, 2006; Wise et al, 2011; Yao et al, 2010). In particular, an increase of BMP6 expression was coincident with the increase of bone formation seen in the base of the tooth crypt.…”

Section: Introductionmentioning

confidence: 99%

Expression of Bone Morphogenetic Protein-6 in Dental Follicle Stem Cells and Its Effect on Osteogenic Differentiation

Yao

He²,

Gutiérrez³

et al. 2013

Cells Tissues Organs

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The dental follicle (DF) plays an essential role in tooth eruption via regulation of bone resorption and bone formation. Bone morphogenetic protein-6 (BMP6) expression in the DF is coincident with bone growth in the tooth crypt. DF stem cells (DFSCs) have been shown to possess strong osteogenic capability. This study aims to determine the expression of BMP6 in DFSCs and to elucidate the role of BMP6 in the osteogenesis of DFSCs. DFSCs and their non-stem cell counterpart, DF cells (DFCs), were obtained from the DFs of rat pups. We showed that expression of BMP6 was significantly higher in the DFSCs than in the DFCs. DFSCs lost osteogenic capability during in vitro expansion, and DFSCs in late passages had reduced BMP6 expression as compared to early passages of DFSCs when they were subjected to osteogenic induction. Addition of exogenous human recombinant BMP6 (hrBMP6) to the osteogenic medium dramatically enhanced the osteogenesis of the late-passage DFSCs. Knockdown of BMP6 by short interfering RNA in the DFSCs in early passages resulted in a decrease in osteogenesis, which could be restored by addition of hrBMP6. We concluded that DFSCs need to express high levels of BMP6 to maintain their osteogenesis capability. Increased BMP6 expression seen in vivo in the DF may reflect the activation of DFSCs for osteogenic differentiation for bone growth during tooth eruption.

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Section: Introductionmentioning

confidence: 99%

Expression of Bone Morphogenetic Protein-6 in Dental Follicle Stem Cells and Its Effect on Osteogenic Differentiation

Yao

He²,

Gutiérrez³

et al. 2013

Cells Tissues Organs

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“…In the posterior palate, a balanced BMP activity is essential for the maintenance of palatal epithelial integrity (Xiong et al, 2009; He et al, 2010). Numerous studies have implicated BMP signaling in many aspects of tooth development, from determination of tooth forming sites and tooth types (Neubüser et al, 1997; Tucker et al, 1998), progression from the bud stage to the cap stage and formation of the enamel knot (Chen et al, 1996; Jernvall et al, 1998; Zhang et al, 2000; Zhao et al, 2000), to tooth root formation and tooth eruption (Yamashiro et al, 2003; Hosoya et al, 2008, Huang et al, 2010, Yao et al, 2010). Among those Bmp genes that are expressed in developing tooth, Bmp4 was suggested to play a central role as a morphogen during early tooth morphogenesis (Vainio et al, 1993; Thesleff and Mikkola, 2002).…”

Section: Introductionmentioning

confidence: 99%

BmprIa is required in mesenchymal tissue and has limited redundant function with BmprIb in tooth and palate development

Lin

Wang

et al. 2011

Developmental Biology

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The BMP signaling plays a pivotal role in the development of craniofacial organs, including the tooth and palate. BmprIa and BmprIb encode two type I BMP receptors that are primarily responsible for BMP signaling transduction. We investigated mesenchymal tissue-specific requirement of BmprIa and its functional redundancy with BmprIb during the development of mouse tooth and palate. BmprIa and BmprIb exhibit partially overlapping and distinct expression patterns in the developing tooth and palatal shelf. Neural crest specific inactivation of BmprIa leads to formation of an unusual type of anterior clefting of the secondary palate, an arrest of tooth development at the bud/early cap stages, and severe hypoplasia of the mandible. Defective tooth and palate development is accompanied by the down-regulation of BMP responsive genes and reduced cell proliferation levels in the palatal and dental mesenchyme. To determine if BmprIb could substitute for BmprIa during tooth and palate development, we expressed a constitutively active form of BmprIb (caBmprIb) in the neural crest cells in which BmprIa was simultaneously inactivated. We found that substitution of BmprIa by caBmprIb in neural rest cells rescues the development of molars and maxillary incisor, but the rescued teeth exhibit a delayed odontoblast and ameloblast differentiation. In contrast, caBmprIb fails to rescue the palatal and mandibular defects including the lack of lower incisors. Our results demonstrate an essential role for BmprIa in the mesenchymal component and a limited functional redundancy between BmprIa and BmprIb in a tissue specific manner during tooth and palate development.

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“…In addition to this Smad-dependent canonical BMP signaling pathway, BMP signaling can also be mediated through Smadindependent mitogen-activated protein kinase (MAPK) pathways, known as non-canonical pathways (Sieber et al, 2009). In the developing mouse tooth, several Bmp genes, including Bmp-2, -3, -4, and -7, are expressed in either epithelial or mesenchymal components (Nie et al, 2006), and BMP activities have been implicated in the determination of tooth-forming site and tooth type (Neubüser et al, 1997;Tucker et al, 1998), progression of tooth development from the bud stage to the cap stage (Chen et al, 1996;Zhang et al, 2000;Zhao et al, 2000), and terminal differentiation and tooth eruption (GluhakHeinrich et al, 2010;Yao et al, 2010). BmprIa and BmprIb have been shown to be expressed, and to function redundantly to a certain extent, in the developing tooth (Li et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

BMP Activity Is Required for Tooth Development from the Lamina to Bud Stage

Wang

Zheng

et al. 2012

J Dent Res

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A supplemental appendix to this article is published electronically only at http://jdr.sagepub.com/supplemental. AbstrActSeveral Bmp genes are expressed in the developing mouse tooth germ from the initiation to the latedifferentiation stages, and play pivotal roles in multiple steps of tooth development. In this study, we investigated the requirement of BMP activity in early tooth development by transgenic overexpression of the extracellular BMP antagonist Noggin. We show that overexpression of Noggin in the dental epithelium at the tooth initiation stage arrests tooth development at the lamina/early-bud stage. This phenotype is coupled with a significantly reduced level of cell proliferation rate and a down-regulation of Cyclin-D1 expression, specifically in the dental epithelium. Despite unaltered expression of genes known to be implicated in early tooth development in the dental mesenchyme and dental epithelium of transgenic embryos, the expression of Pitx2, a molecular marker for the dental epithelium, became down-regulated, suggesting the loss of odontogenic fate in the transgenic dental epithelium. Our results reveal a novel role for BMP signaling in the progression of tooth development from the lamina stage to the bud stage by regulating cell proliferation and by maintaining odontogenic fate of the dental epithelium.

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TNF-α Upregulates Expression of BMP-2 and BMP-3 Genes in the Rat Dental Follicle—Implications for Tooth Eruption

Cited by 29 publications

References 32 publications

Expression of Bone Morphogenetic Protein-6 in Dental Follicle Stem Cells and Its Effect on Osteogenic Differentiation

Expression of Bone Morphogenetic Protein-6 in Dental Follicle Stem Cells and Its Effect on Osteogenic Differentiation

BmprIa is required in mesenchymal tissue and has limited redundant function with BmprIb in tooth and palate development

BMP Activity Is Required for Tooth Development from the Lamina to Bud Stage

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