TNF-α induced down-regulation of lysyl oxidase family in anterior cruciate ligament and medial collateral ligament fibroblasts

Xie, Jing; Jiang, Jiahuan; Huang, Wei; Zhang, Yanjun; Xu, Chunming; Wang, Chunli; Yin, Lin; Chen, Peter C. Y.; Sung, K.L. Paul

doi:10.1016/j.knee.2012.12.015

Cited by 19 publications

(16 citation statements)

References 32 publications

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“…Previous studies revealed that both the ACLfs and SCs are very susceptible to TNF-α in regulating the expressions of MMPs and LOXs. 8,14 TNF-α could destroy the balance of ECM through various ways, including inhibition of LOXs expression in the ACLf, promotion of MMPs expression in the ACLfs, and perisurrounded SCs. Previous data have proved that both ACLfs and SCs in the presence of TNF-α has high MMP activities, which have been hypothesized to primarily be responsible for the poor self-healing of cruciate ligaments in monoculture condition.…”

Section: Discussionmentioning

confidence: 99%

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Expression of LOXs and MMP-1, 2, 3 by ACL Fibroblasts and Synoviocytes Impact of Coculture and TNF-α

Wang

Chi

et al. 2018

J Knee Surg

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This study aims to confirm the effects of synoviocytes (SCs) on regulating lysyl oxidases (LOXs) and matrix metalloproteinase (MMP)-1, 2, 3 in the normal and injured anterior cruciate ligament (ACL) fibroblasts response to tumor necrosis factor-α(TNF-α). The gene and protein expression levels of LOXs and MMP-1, 2, 3 in SCs cocultured ACL fibroblasts (ACLfs) induced by TNF-α and mechanical injury were analyzed by real-time polymerase chain reaction (PCR) and western bolting; the MMP-2 activity were analyzed by zymography. The results exhibited that TNF-α alone slightly downregulated the expressions of LOXs and upregulated the expression of MMP-1, 2, 3 in both normal and injured ACL fibroblasts. The decrease of LOXs and increase of MMP-1, 2, 3 in ACLfs response to TNF-α were further promoted by coculture. Taken together, these results show for the first time that the crosstalk between ACLfs and SCs could modulate the LOXs and MMP-1, 2, 3 synthesis in ACLfs in the presence of TNF-α. Accumulation of MMPs in the isolated fluid-containing space not only disrupts the balance of ACL healing, but also increases cartilage degradation and accelerates osteoarthritis (OA) in injured joint. Based on this mechanism, targeting inhibition of MMPs could provide a promising therapeutic strategy for acute ligament injury.

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Section: Discussionmentioning

confidence: 99%

“…13 Previous studies have demonstrated that the ACLfs and synovial fibroblasts are very sensitive to TNF-α in regulating repair behavior of injured ACL. 14,15 However, very few effects are known to the TNF-α responsiveness of ACLfs in SCs' coculture condition.…”

mentioning

confidence: 99%

Expression of LOXs and MMP-1, 2, 3 by ACL Fibroblasts and Synoviocytes Impact of Coculture and TNF-α

Wang

Chi

et al. 2018

J Knee Surg

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“…In fact, there are some recent studies that points-out to the idea of the self-healing of the ACL breakage, although this capacity is probably limited (Fujimoto, Sumen, Ochi, & Ikuta, 2002;Kohl et al, 2013) and may be influenced by acute phase inflammatory mediators such as the tumor necrosis factor alpha (TNF-α) (Xie et al, 2014). The healing efficiency is mediated by the fibroblastic lysyl oxidase enzyme, which function is to catalyze the cross-linking between the collagen and the elastin and whose activity increases with the levels of TNF-α (Xie et al, 2014). As in our experiments, the dissection of the ACL and the repair was done in the same surgical procedure; this acute-phase healing mechanism above described could explain that both neo-formed ACL had the same macroscopic aspect.…”

Section: Discussionmentioning

confidence: 99%

The Use of Autologous Fibroblasts for the Reconstruction of the Anterior Cruciate Ligament Tears

Guillén-García¹,

Rodríguez‐Iñigo²,

Guillén-Vicente³

et al. 2015

IJB

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Surgical reconstruction using an autologous or allogenic allograft is the treatment of choice for anterior cruciate ligament (ACL) tear repair. We have checking the treatment of ACL tears with in vitro cultured autologous fibroblasts on porcine type I/III collagen membranes, in 12 sheep randomly assigned to be treated with collagen membrane without cells or with porcine membrane plus 5 million autologous fibroblasts per cm 2 . The animals underwent a first surgery in which a biopsy of the ACL was taken, and a second one in which the membrane was implanted alone (N=6) or with the fibroblasts (N=6) isolated from the biopsy. After 16 weeks, the animals were sacrificed and samples of healthy and repaired ACL were taken for histological and gene expression of type I collagen (COL1), MMP-13 (matrix metallopeptidase 13), and tenascin-C (TNC) studies. The architecture of normal ACL was not conserved either in the ACL treated with the membrane with or without cells. However, a higher degree of peripheral vascularization was observed in the ACL treated with membrane plus fibroblasts than in those treated with membrane alone. Cellularity and nucleus volume were significantly higher in the center or the periphery of the neo-formed ACL than in the control ACL. No differences between the ACL treated with membrane alone or with membrane plus cells were observed in the relative COL1, MMP-13 and tenascin-C expression. Fibroblasts embedded in porcine type I/III membranes probably enhance vascularization of the graft, so it could be a promising tool for the treatment of ACL tears.

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“…Our previous studies elucidated that the IL-1 could increase LOX expressions in the ligament of human knee, 33 while the TNF-could decrease the LOX expressions. 34 The TGF-1 is an important growth factor in tissue remodeling that can enhance LOX expressions to promote the wound healing in ligaments. 35 In the present study we elucidated the expressions of LOXs induced by different dosages of the TNF-.…”

Section: Tnf-induced the Expressions Of Loxs In Timentioning

confidence: 99%

Regulation of Extracellular Matrix Remodeling Proteins by Osteoblasts in Titanium Nanoparticle-Induced Aseptic Loosening Model

Xie¹,

Hou²,

Fu³

et al. 2015

j biomed nanotechnol

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Titanium (Ti)-wear particles, formed at the bone-implant interface, are responsible for aseptic loosening, which is a main cause of total joint replacement failure. There have been many studies on Ti particle-induced function changes in mono-cultured osteoblasts and synovial cells. However, little is known on extracellular matrix remodeling displayed by osteoblasts when in coexistence with Synovial cells. To further mimic the bone-implant interface environment, we firstly established a nanoscaled-Ti particle-induced aseptic loosening system by co-culturing osteoblasts and Synovial cells. We then explored the impact of the Synovial cells on Ti particle-engulfed osteoblasts in the mimicked flamed niche. The matrix metalloproteinases and lysyl oxidases expression levels, two protein families which are critical in osseointegration, were examined under induction by tumor necrosis factor-alpha. It was found that the co-culture between the osteoblasts and Synovial cells markedly increased the migration and proliferation of the osteoblasts, even in the Ti-particle engulfed osteoblasts. Importantly, the Ti-particle engulfed osteoblasts, induced by TNF-alpha after the co-culture, enhanced the release of the matrix metalloproteinases and reduced the expressions of lysyl oxidases. The regulation of extracellular matrix remodeling at the protein level was further assessed by investigations on gene expression of the matrix metalloproteinases and lysyl oxidases, which also suggested that the regulation started at the genetic level. Our research work has therefore revealed the critical role of multi cell-type interactions in the extracellular matrix remodeling within the peri-prosthetic tissues, which provides new insights on aseptic loosening and brings new clues about incomplete osseointegration between the implantation materials and their surrounding bones.

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TNF-α induced down-regulation of lysyl oxidase family in anterior cruciate ligament and medial collateral ligament fibroblasts

Cited by 19 publications

References 32 publications

Expression of LOXs and MMP-1, 2, 3 by ACL Fibroblasts and Synoviocytes Impact of Coculture and TNF-α

Expression of LOXs and MMP-1, 2, 3 by ACL Fibroblasts and Synoviocytes Impact of Coculture and TNF-α

The Use of Autologous Fibroblasts for the Reconstruction of the Anterior Cruciate Ligament Tears

Regulation of Extracellular Matrix Remodeling Proteins by Osteoblasts in Titanium Nanoparticle-Induced Aseptic Loosening Model

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