TNF-α Contributes To The Development Of Asthma By Enhancing IL-23/Th17 And Th2 Immune Responses

Lee, Hyeongrae; Jeon, E.; Bang, Bo; Shim, Eun Jin; Kwon, Jihoon; Kim, T.; Jung, Jae-Chul; Lee, S.; Kim, K.; Kim, S.; Kang, Hyun Mi; Park, H.; Chang, Yoon Seok; Cho, S.; Min, Kyung Up; Kim, Y.

doi:10.1016/j.jaci.2009.12.414

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“…Th17 and TNF-α are important factors for mediating inflammation during the development of inflammatory disease [26,27]. TNF-α contributes to the development of asthma by promoting Th17 expression during immune responses [28]. A previous study showed that Th17 and TNF-α in the cells of bone marrow can lead to osteoclast differentiation and bone destruction in IBD [29].…”

Section: Discussionmentioning

confidence: 99%

miR-802 participates in the inflammatory process of inflammatory bowel disease by suppressing SOCS5

et al. 2020

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The present study aims to reveal the detailed molecular mechanism of microRNA (miR)-802 in the progression of inflammatory bowel disease (IBD). IBD tissues were obtained from IBD patients, followed by CD4+ cells isolation. Then, qRT-PCR and ELISA were used to detect the expression of miR-802, suppressor of cytokine signaling 5 (SOCS5), interleukin (IL)-17A and tumor necrosis factor (TNF)-α. Transfection of miR-802 mimics and miR-802 inhibitor in CD4+ cells was detected by Western blot. TargetScan and luciferase reporter assay were used to detect the relationship between SOCS5 and miR-802. Finally, colitis mice model was established to verify whether miR-802 inhibitor was involved in the protective effect of colonic mucosa. The miR-802 was highly expressed in inflamed mucosa and PBMC cells of IBD. The highest expression of miR-802 was observed in CD4+ T cells based on different immune cell subsets analysis. SOCS5 was the target gene of miR-802. The mice model experiments showed that blockade of miR-802 could alleviate mice colitis. Our study suggests that up-regulation of miR-802 plays an important role in inflammatory process of IBD via targeting SOCS5. Moreover, the differentiation of Th17 and secretion of TNF-α in IBD could be stimulated by miR-802.

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Section: Discussionmentioning

confidence: 99%