TNF induces neutrophil adhesion via formin-dependent cytoskeletal reorganization and activation of β-integrin function

Silveira, Angélica Aparecida Antoniellis; Dominical, Venina Marcela; Almeida, Camila B.; Chweih, Hanan; Ferreira, Wilson A.; Vicente, Cristina Pontes; Costa, Fábio Trindade Maranhão; Werneck, Cláudio C.; Costa, Fernando Ferreira; Conran, Nicola

doi:10.1189/jlb.3a0916-388rr

Cited by 29 publications

(15 citation statements)

References 70 publications

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“…The cytokine response of TNF‐α elevation observed after S. elongatus injection was not statistically significant but nevertheless noteworthy. This cytokine typically increases in the setting of inflammation and is crucial for neutrophil migration and adhesion (Silveira et al ., 2018). Its increase 4 h following S. elongatus injection preceded the increase in neutrophils seen 48 h after injection in these rats.…”

Section: Discussionmentioning

confidence: 99%

Safety of photosynthetic Synechococcus elongatus for in vivo cyanobacteria–mammalian symbiotic therapeutics

Williams

Wang

Paulsen

et al. 2020

Microbial Biotechnology

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The cyanobacterium Synechococcus elongatus (SE) has been shown to rescue ischaemic heart muscle after myocardial infarction by photosynthetic oxygen production. Here, we investigated SE toxicity and hypothesized that systemic SE exposure does not elicit a significant immune response in rats. Wistar rats intravenously received SE (n = 12), sterile saline (n = 12) or E. coli lipopolysaccharide (LPS, n = 4), and a subset (8 SE, 8 saline) received a repeat injection 4 weeks later. At baseline, 4 h, 24 h, 48 h, 8 days and 4 weeks after injection, clinical assessments, blood cultures, blood counts, lymphocyte phenotypes, liver function tests, proinflammatory cytokines and immunoglobulins were assessed. Across all metrics, SE rats responded comparably to saline controls, displaying no clinically significant immune response. As expected, LPS rats exhibited severe immunological responses. Systemic SE administration does not induce sepsis or toxicity in rats, thereby supporting the safety of cyanobacteria-mammalian symbiotic therapeutics using this organism.

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Section: Discussionmentioning

confidence: 99%

Safety of photosynthetic Synechococcus elongatus for in vivo cyanobacteria–mammalian symbiotic therapeutics

Williams

Wang

Paulsen

et al. 2020

Microbial Biotechnology

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“…While TNF-a has not been directly implicated in this polarization, its role in TGF-b pathway and the other pathways in which it modulates neutrophil activity described below highlights the importance of neutrophil-neoplasm interaction. In normal vasculature in vivo, TNF-a increases neutrophil recruitment and endothelial adhesion via cytoskeletal remodeling (96,97). TNF-a also has a "priming" effect on neutrophils, causing them to be more responsive to stimuli (98).…”

Section: Neutrophilsmentioning

confidence: 99%

The Role of Tumor Necrosis Factor in Manipulating the Immunological Response of Tumor Microenvironment

Laha¹,

Grant²,

Mishra³

et al. 2021

Front. Immunol.

102

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The tumor microenvironment (TME) is an intricate system within solid neoplasms. In this review, we aim to provide an updated insight into the TME with a focus on the effects of tumor necrosis factor-α (TNF-α) on its various components and the use of TNF-α to improve the efficiency of drug delivery. The TME comprises the supporting structure of the tumor, such as its extracellular matrix and vasculature. In addition to cancer cells and cancer stem cells, the TME contains various other cell types, including pericytes, tumor-associated fibroblasts, smooth muscle cells, and immune cells. These cells produce signaling molecules such as growth factors, cytokines, hormones, and extracellular matrix proteins. This review summarizes the intricate balance between pro-oncogenic and tumor-suppressive functions that various non-tumor cells within the TME exert. We focused on the interaction between tumor cells and immune cells in the TME that plays an essential role in regulating the immune response, tumorigenesis, invasion, and metastasis. The multifunctional cytokine, TNF-α, plays essential roles in diverse cellular events within the TME. The uses of TNF-α in cancer treatment and to facilitate cancer drug delivery are discussed. The effects of TNF-α on tumor neovasculature and tumor interstitial fluid pressure that improve treatment efficacy are summarized.

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“…This cytokine is suggested to be generated as an early consequence of ischemia-reperfusion [207] and has potent effects on both leukocytes and endothelial cells. In neutrophils, TNF-α stimulates the surface expression of β−2 integrins [208], in turn augmenting their adhesion to the blood vessel wall [208] and interactions with other cells via NF-κB and MAPK signaling [209]. Furthermore, TNF-α degrades the endothelial glycocalyx (shown to be reduced in SCD patients) [210, 211], alters endothelium-derived NO bioavailability [212] and upregulates adhesion molecule expression on the endothelium [213], with a monocyte-dependent TNF-endothelial activation axis being recently described in sickle mice [207].…”

Section: Chronic Inflammatory Mechanisms In Sickle Cell Diseasementioning

confidence: 99%

Inflammation in sickle cell disease

Conran

Belcher

2018

Self Cite

182

178

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The primary β-globin gene mutation that causes sickle cell disease (SCD) has significant pathophysiological consequences that result in hemolytic events and the induction of the inflammatory processes that ultimately lead to vaso-occlusion. In addition to their role in the initiation of the acute painful vaso-occlusive episodes that are characteristic of SCD, inflammatory processes are also key components of many of the complications of the disease including autosplenectomy, acute chest syndrome, pulmonary hypertension, leg ulcers, nephropathy and stroke. We, herein, discuss the events that trigger inflammation in the disease, as well as the mechanisms, inflammatory molecules and cells that propagate these inflammatory processes. Given the central role that inflammation plays in SCD pathophysiology, many of the therapeutic approaches currently under pre-clinical and clinical development for the treatment of SCD endeavor to counter aspects or specific molecules of these inflammatory processes and it is possible that, in the future, we will see anti-inflammatory drugs being used either together with, or in place of, hydroxyurea in those SCD patients for whom hematopoietic stem cell transplants and evolving gene therapies are not a viable option.

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TNF induces neutrophil adhesion via formin-dependent cytoskeletal reorganization and activation of β-integrin function

Cited by 29 publications

References 70 publications

Safety of photosynthetic Synechococcus elongatus for in vivo cyanobacteria–mammalian symbiotic therapeutics

Safety of photosynthetic Synechococcus elongatus for in vivo cyanobacteria–mammalian symbiotic therapeutics

The Role of Tumor Necrosis Factor in Manipulating the Immunological Response of Tumor Microenvironment

Inflammation in sickle cell disease

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