Tn
            <i>6450</i>
            , a Novel Multidrug Resistance Transposon Characterized in a Proteus mirabilis Isolate from Chicken in China

Chen, Yanpeng; Lei, Changwei; Kong, Linghan; Zeng, Jinxin; Zhang, Xiuzhong; Liu, Bihui; Li, Yang; Xiang, Rong; Wang, Yongxiang; Chen, Danyu; Zhang, Anyun; Wang, Hongning

doi:10.1128/aac.02192-17

Cited by 24 publications

(17 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No plasmid replicon was identified, but pHNCF44W_NDM-1 was very similar to plasmid plas1.1.2 (CP047114) from Proteus mirabilis of swine origin; it differed mainly in the bla NDM– 1 -bearing region ( Figure 2 ). The bla NDM– 1 gene and other resistance genes were found in a 65-kb MDR region, showing high similarity to transposon Tn 6450 but with bla DHA– 1 replaced by bla NDM– 1 ( Chen et al, 2018 ), designated as Tn 6450b here ( Figure 1B ). Other similar plasmids or transposons were found in the nr database, including p16Pre36-NDM from Providencia rettgeri , pHFK418-NDM from P. mirabilis , pPrY2001 from P. rettgeri , p16Pre36-NDM from P. rettgeri , p06-1619-1 from P. rettgeri , and Tn 6450 from P. mirabilis .…”

Section: Resultsmentioning

confidence: 99%

Emergence of Carbapenem- and Tigecycline-Resistant Proteus cibarius of Animal Origin

Wang

Peng

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

The emergence of tet(X) and carbapenemase genes in Enterobacterales pose significant challenges to the treatment of infectious diseases. Convergence of these two categories of genes in an individual pathogen would deteriorate the antimicrobial resistance (AMR) crisis furthermore. Here, tigecycline-resistant Enterobacterales strains were isolated and detected with carbapenemase genes, characterized by antimicrobial susceptibility testing, PCR, conjugation assay, whole genome sequencing, and bioinformatics analysis. Three tigecycline-resistant isolates consisting of one plasmid-mediated tet(X4)bearing Escherichia fergusonii and two chromosomal tet(X6)-bearing Proteus cibarius were recovered from chicken feces. The tet(X4) was located on a conjugative IncX1 plasmid pHNCF11W-tetX4 encoding the identical structure as reported tet(X4)-bearing IncX1 plasmids in Escherichia coli. Among two P. cibarius strains, tet(X6) was located on two similar chromosomal MDR regions with genetic contexts IS26-aac(3)-IVa-aph(4)-Ia-ISEc59-tnpA-tet(X6)-orf-orf-ISCR2-virD2-floR-ISCR2-glmM-sul2 and IS26-aac(3)-IVaaph(4)-Ia-ISEc59-tnpA-tet(X6)-orf-orf-ISCR2-glmM-sul2. Apart from tet(X6), P. cibarius HNCF44W harbored a novel transposon Tn6450b positive for bla NDM−1 on a conjugative plasmid. This study probed the genomic basis of three tet(X)-bearing, tigecycline-resistant strains, one of which coharbored bla NDM−1 and tet(X6), and identified P. cibarius as the important reservoir of tet(X6) variants. Emergence of P. cibarius encoding both bla NDM−1 and tet(X6) reveals a potential public health risk.

show abstract

Section: Resultsmentioning

confidence: 99%

Emergence of Carbapenem- and Tigecycline-Resistant Proteus cibarius of Animal Origin

Wang

Peng

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Tn6765 was highly homologous with Tn6450 located on the chromosome detected by Chen (33). The similarity of nucleic acid sequence was more than 99%, and the coverage was 96%.…”

Section: Antimicrobial Resistance Phenotypesmentioning

confidence: 83%

“…41Ominously, like its distant relative Tn7, Tn6765 may exhibit the potential to jump bacterial species readily. To make matters worse, Tn6450 comes from a chicken source (33) and Tn6765 from a pig source, suggesting that Tn7-like can be transmitted from one animal to another with bacterial hosts.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Tn7-like transposons and its related antibiotic resistance among Enterobacteriaceae from livestock and poultry in China

He¹,

Li²,

Cui³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: This study was aimed to investigate the prevalence and structure of Tn7-like in Enterobacteriaceae from livestock and poultry as well as their possible role as reservoir of antibiotic resistance genes (ARGs). Methods:Polymerase chain reaction (PCR) and DNA sequencing analyses were used for the characterization of Tn7-like, associated integrons and ARGs. The antimicrobial resistance profile of the isolates was examined by using disc diffusion test.Results: Three hundred and seventy-eight Tn7-like-positive strains of Enterobacteriaceae were isolated, and included E. coli (128), Proteus(150), K. pneumonia(17), Salmonella(13), M. morganii (21) and A. baumannii(1), wherein high resistance was observed for Trimethoprim/Sulfamethoxazole and Streptomycin, and fifty percent of the strains were multidrug-resistant. Integrons class 2 were detected in all of the isolates and there are high frequency mutation sites especially in 535, a stop mutation. Variable region of class 2 integrons carried same gene cassettes, namely aadA1-sat2-dfrA1.From the 378 isolated strains, we found a new type of Tn7-like on a plasmid, named Tn6765. Conclusions:These findings proved that the Tn7-like can contribute to the horizontal transmission of antibiotic resistant genes in livestock and poultry. As potential vessels for antibiotic resistance genes (ARGs), Tn7-like could not be ignored due to their efficient transfer ability in environments.

show abstract

“…Tn 6765 was highly homologous with Tn 6450 (GenBank accession number MF805806), which was located on the chromosome detected by Chen et al (2018) . The similarity of nucleic acid sequence was more than 99%, and the coverage was 96%.…”

Section: Discussionmentioning

confidence: 99%

“…A common form of resistance dissemination in Enterobacterales is mediated by transposons. Tn 7 -like transposons, as the Tn 7 transposon derivatives, have been found in several species of the Enterobacterales order, such as Proteus mirabilis and Morganella morganii ( Chen et al, 2018 , 2019 ). This kind of transposons carries a great diversity of antimicrobial resistance genes (ARGs).…”

Section: Introductionmentioning

confidence: 99%

Detection of Tn7-Like Transposons and Antibiotic Resistance in Enterobacterales From Animals Used for Food Production With Identification of Three Novel Transposons Tn6813, Tn6814, and Tn6765

Cui

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

Enterobacterales are widely distributed in the gastro-intestinal system of animals and may cause opportunistic infections. Worse still, multidrug-resistant Enterobacterales also poses a serious threat to public health. Tn7-like transposons have been found in several species of the Enterobacterales order and play an important role in dissemination of antibiotic resistance. This study aimed to investigate the distribution and genetic characterization of Tn7-like transposons in Enterobacterales isolates from food animals and their association with antibiotic resistance. Enterobacterales isolated from the samples were identified and classified according to the 16S rDNA sequence. Tn7-like transposons and associated integrons were detected by polymerase chain reaction (PCR) and sequencing. The antibiotic resistance of each Tn7-like transposon positive isolate was detected according to the Kirby-Bauer disk diffusion method. Then, six representative strains were selected to study the genetic environment by whole-genome sequencing (WGS). In total, we isolated 377 Tn7-like transposons positive strains of Enterobacterales. Class 2 integrons were detected in 99.5% of the isolates, and there were high frequency mutation sites especially in base 535, a stop mutation. Many isolates (54.9%) were multidrug-resistant and observed high resistance rates to trimethoprim/sulfamethoxazole and streptomycin. Among these strains, we found three new types of Tn7-like transposons, named Tn6813, Tn6814, and Tn6765. This is the first comprehensive survey that shows Tn7-like transposons in Enterobacterales from animals used for food production in different regions of China. This study also provides an insight into the horizontal transfer of resistance genes associated with Tn7-like transposons.

show abstract

Tn 6450 , a Novel Multidrug Resistance Transposon Characterized in a Proteus mirabilis Isolate from Chicken in China

Cited by 24 publications

References 19 publications

Emergence of Carbapenem- and Tigecycline-Resistant Proteus cibarius of Animal Origin

Emergence of Carbapenem- and Tigecycline-Resistant Proteus cibarius of Animal Origin

Characterization of Tn7-like transposons and its related antibiotic resistance among Enterobacteriaceae from livestock and poultry in China

Detection of Tn7-Like Transposons and Antibiotic Resistance in Enterobacterales From Animals Used for Food Production With Identification of Three Novel Transposons Tn6813, Tn6814, and Tn6765

Contact Info

Product

Resources

About